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Hsa-miRNA-31 regulates epithelial cell barrier function by inhibiting TNFSF15 expression
Corresponding Author(s) : X Nan
Cellular and Molecular Biology,
Vol. 62 No. 4: Issue 4
Abstract
Ulcerative colitis (UC) is characterized by epithelial barrier disruption and alterations in immune regulation but with the etiology unknown. MicroRNA-31 is the most consistent differentially expressed miRNA in ulcerative colitis tissue. The aim of this project is to study the important roles of miRNA-31 in regulation of intestinal epithelial barrier function. We found that expression of miRNA-31 is proportional to the proliferation of Caco2-BBE cells and overexpression of miRNA-31 can increase its trans-epithelial resistance (TER) by decreasing the transepithelial permeability. miRNA-31 can directly bind to the 3-UTR of TNFSF15, thereafter negatively regulating its expression in Caco2-BBE cells. BrdU and TUNEL analysis demonstrated that transfection of miRNA-31 stimulates proliferation or apoptosis-resistance. Taken together, these results revealed a novel mechanism underlying the regulation of epithelial barrier function by miRNA-31 during its regulation on proliferation of epithelial cells.
Keywords
TNFSF15
miRNA-31
epithelial proliferation
barrier function.
Nan, X., Qin, S., Yuan, Z., Li, Y., Zhang, J., Li, C., Tan, X., & Yan, Y. (2016). Hsa-miRNA-31 regulates epithelial cell barrier function by inhibiting TNFSF15 expression. Cellular and Molecular Biology, 62(4), 104–110. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/848
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