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Vol. 69 No. 12: New discoveries in gene expression and mutation

Copyright (c) 2023 Jian Wang, Yan Li, Zhe Xu, Min Wang, Jie Cheng, Shan Gao, Weiyu Kong, Lijun Qu

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LncRNA DCST1-AS1 drives the malignant progression of pediatric acute myeloid leukemia via regulating p53 methylation

LncRNA DCST1-AS1 and pediatric AML
https://doi.org/10.14715/cmb/2023.69.12.32
Jian Wang
Department of Pediatric Hematology and Tumor, Anhui Provincial Children’s Hospital, Hefei, China
Yan Li
Department of Pediatric Hematology and Tumor, Anhui Provincial Children’s Hospital, Hefei, China
Zhe Xu
Department of Pediatric Hematology and Tumor, Anhui Provincial Children’s Hospital, Hefei, China
Min Wang
Department of Pediatric Hematology and Tumor, Anhui Provincial Children’s Hospital, Hefei, China
Jie Cheng
Department of Pediatric Hematology and Tumor, Anhui Provincial Children’s Hospital, Hefei, China
Shan Gao
Department of Pediatric Hematology and Tumor, Anhui Provincial Children’s Hospital, Hefei, China
Weiyu Kong
Department of Pediatric Hematology and Tumor, Anhui Provincial Children’s Hospital, Hefei, China
Lijun Qu
Department of Pediatric Hematology and Tumor, Anhui Provincial Children’s Hospital, Hefei, China

Corresponding Author(s) : Lijun Qu

qulijun12345@163.com

Cellular and Molecular Biology, Vol. 69 No. 12: New discoveries in gene expression and mutation

Abstract

To uncover the biological role of lncRNA DCST1-AS1 in the process of pediatric AML and its regulatory effect on p53 methylation. Serum level of DCST1-AS1 in AML children and healthy participants was detected by qRT-PCR. The role of DCST1-AS1 in mediating biological functions of AML193 and U937 cells was assessed by functional experiments. p53 methylation level was examined. Through BSP, MSP and dual-luciferase reporter assay, the regulatory effect of DCST1-AS1 on p53 methylation was explored. The correlation between DCST1-AS1 and p53 in the serum of AML children was assessed. Serum level of DCST1-AS1 was higher in AML children than in healthy subjects. Knockdown of DCST1-AS1 decreased proliferative and migratory rates in AML193 and U937 cells. DCST1-AS1 was able to induce methylation of p53 promoter. P53 was markedly upregulated by the knockdown of DCST1-AS1, presenting a negative correlation. LncRNA DCST1-AS1 drives the malignant progression of pediatric AML through inducing methylation of the p53 promoter.

Keywords

LncRNA DCST1-AS1 p53 AML
Wang, J. ., Li, Y. ., Xu, Z. ., Wang, M. ., Cheng, J. ., Gao, S. ., Kong, W. ., & Qu, L. (2023). LncRNA DCST1-AS1 drives the malignant progression of pediatric acute myeloid leukemia via regulating p53 methylation: LncRNA DCST1-AS1 and pediatric AML. Cellular and Molecular Biology, 69(12), 201–204. https://doi.org/10.14715/cmb/2023.69.12.32
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