Issue
Mitochondrial gene cytochrome c oxidase I (CO1) used for molecular identification of Bactrocera zonata in Pakistan
Corresponding Author(s) : Khadim Hussain
Cellular and Molecular Biology,
Vol. 65 No. 2: Issue 2
Abstract
Bactrocera zonata is fruit pest mostly attacked on peach and cause heavy destruction in production of peach fruits by sucking their juice. For their management, we start to detect them on basis of their molecular characterization. As mitochondrial genome encodes a gene COI used as biomarker for identification of eukaryotes including insects. In present study, we amplified COI gene and cloned into pTZ57R/T vector (Fermentas). Cloned gene was confirmed through restriction analysis and sequenced through its entirety on both strands from Macrogen (South Korea) by Sanger sequencing method. Different computational tools were utilized for comparative analysis of sequence with other related sequences retrieved from databases. Related species were identified through phylogenetic analysis using Mega 7 tool. Pairwise sequence alignment showed the sequence identity about 96% with Bactrocera zonata. By identifying the pests with more authentic molecular biomarker may help the research to control them more effectively in future.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- Iwahashi, O. and W. Routhier, Aedeagal length and its variation of the peach fruit fly, Bactrocera zonata (Saunders)(Diptera: Tephritidae), which recently invaded Egypt. Applied Entomology and Zoology 2001; 36: 13-17.
- Khlaywi, S.A., et al., Identification of Peach Fruit Fly, Bactrocera Zonata (Saunders) in Iraq. 2017.
- Agarwal, M., K. Pramod, and K. Vinod, Population suppression of Bactrocera dorsalis (Hendel) by Bactrocera zonata (Saunders)(Diptera: Tephritidae) in North Bihar. Shashpa, 1999. 6(2): p. 189-191.
- White, I.M. and M.M. Elson-Harris, Fruit flies of economic significance: their identification and bionomics. 1992: CAB International.
- Choudhary, J.S., et al., The mitochondrial genome of the peach fruit fly, Bactrocera zonata (Saunders)(Diptera: Tephritidae): Complete DNA sequence, genome organization, and phylogenetic analysis with other tephritids using next generation DNA sequencing. Gene, 2015; 569: 191-202.
- Smith, M.A., et al., DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proceedings of the National Academy of Sciences of the United States of America 2006; 103: 3657-3662.
- Hebert, P.D., A. Cywinska, and S.L. Ball, Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences, 2003; 270: 313-321.
- Park, D.-S., et al., Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera). Plos one, 2011; 6: e18749.
- Memon, N., et al., On the use of DNA sequences for determining the species limits of a polymorphic new species in the stink bug genus Halys (Heteroptera: Pentatomidae) from Pakistan. Systematic Entomology, 2006; 31: 703-710.
- Khan, A., et al., Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene. Cellular and molecular biology (Noisy-le-Grand, France), 2017; 63: 59-63.
- Ashfaq, M., et al., DNA barcoding of Bemisia tabaci complex (Hemiptera: Aleyrodidae) reveals southerly expansion of the dominant whitefly species on cotton in Pakistan. PLoS One, 2014; 9: e104485.
- Tamura, K., M. Nei, and S. Kumar, Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences of the United States of America, 2004; 101: 11030-11035.
References
Iwahashi, O. and W. Routhier, Aedeagal length and its variation of the peach fruit fly, Bactrocera zonata (Saunders)(Diptera: Tephritidae), which recently invaded Egypt. Applied Entomology and Zoology 2001; 36: 13-17.
Khlaywi, S.A., et al., Identification of Peach Fruit Fly, Bactrocera Zonata (Saunders) in Iraq. 2017.
Agarwal, M., K. Pramod, and K. Vinod, Population suppression of Bactrocera dorsalis (Hendel) by Bactrocera zonata (Saunders)(Diptera: Tephritidae) in North Bihar. Shashpa, 1999. 6(2): p. 189-191.
White, I.M. and M.M. Elson-Harris, Fruit flies of economic significance: their identification and bionomics. 1992: CAB International.
Choudhary, J.S., et al., The mitochondrial genome of the peach fruit fly, Bactrocera zonata (Saunders)(Diptera: Tephritidae): Complete DNA sequence, genome organization, and phylogenetic analysis with other tephritids using next generation DNA sequencing. Gene, 2015; 569: 191-202.
Smith, M.A., et al., DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proceedings of the National Academy of Sciences of the United States of America 2006; 103: 3657-3662.
Hebert, P.D., A. Cywinska, and S.L. Ball, Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences, 2003; 270: 313-321.
Park, D.-S., et al., Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera). Plos one, 2011; 6: e18749.
Memon, N., et al., On the use of DNA sequences for determining the species limits of a polymorphic new species in the stink bug genus Halys (Heteroptera: Pentatomidae) from Pakistan. Systematic Entomology, 2006; 31: 703-710.
Khan, A., et al., Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene. Cellular and molecular biology (Noisy-le-Grand, France), 2017; 63: 59-63.
Ashfaq, M., et al., DNA barcoding of Bemisia tabaci complex (Hemiptera: Aleyrodidae) reveals southerly expansion of the dominant whitefly species on cotton in Pakistan. PLoS One, 2014; 9: e104485.
Tamura, K., M. Nei, and S. Kumar, Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences of the United States of America, 2004; 101: 11030-11035.
