Resistant/susceptible classification of respiratory tract pathogenic bacteria based on volatile organic compounds profiling

Najmeh Karami; Hassan Rezadoost; Fateme Mirzajani; Abdollah Karimi; Alireza Ghassempour; Atousa Aliahmadi; Fatemeh Fallah

doi:10.14715/cmb/2018.64.9.2

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Vol. 64 No. 9: Issue 9

Issue Published : July 19, 2018

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Resistant/susceptible classification of respiratory tract pathogenic bacteria based on volatile organic compounds profiling

https://doi.org/10.14715/cmb/2018.64.9.2

Najmeh Karami

Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Hassan Rezadoost

Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Tehran, Iran.

Fateme Mirzajani

Protein Research Center, Shahid Beheshti University, G.C., Tehran, Iran.

Abdollah Karimi

Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Alireza Ghassempour

Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Tehran, Iran.

Atousa Aliahmadi

Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Tehran, Iran.

Fatemeh Fallah

Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Corresponding Author(s) : Fatemeh Fallah

dr.fafallah@gmail.com

Cellular and Molecular Biology, Vol. 64 No. 9: Issue 9
Article Published : June 30, 2018

Abstract

Resistance to antibiotics is an emerging and growing threat. To address this threat, attempts are being made by researchers to identify the Volatile Organic Compounds (VOCs) of bacteria. It is believed that unique combinations could be found among the VOCs produced by each microorganism. The current study aimed to identify and compare the VOCs of antibiotic-resistant and standard strains of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae. A polymer of divinylbenzene /carboxen /polydimethylsiloxane was applied for absorption of volatile compounds in headspace bacterial samples in form of a solid phase micro-extraction fiber holder. Gas chromatography-mass spectrometry technique was used for identification of volatile compounds. The analysis of the VOCs indicated that some VOCs appeared only in standard strains while others were common only among resistant strains. Exclusive VOCs to a specific strain were also detected. This study demonstrated that resistant strains of bacteria produced VOCs that were different from those of the standard strains. In addition, VOCs released by bacteria after passing the logarithmic growth phase showed no significant differences. The identification of VOCs can be a precise way to differentiate bacterial species, also it can be said that the VOCs produced by different pathogenic microorganisms can be the suitable biomarkers for their detection.

Keywords

Resistant strains Standard strains Volatile organic compounds Solid phase micro-extraction.

Karami, N., Rezadoost, H., Mirzajani, F., Karimi, A., Ghassempour, A., Aliahmadi, A., & Fallah, F. (2018). Resistant/susceptible classification of respiratory tract pathogenic bacteria based on volatile organic compounds profiling. Cellular and Molecular Biology, 64(9), 6–15. https://doi.org/10.14715/cmb/2018.64.9.2

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References

References
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Roberts RR, Hota B, Ahmad I et al. Hospital and societal costs of antimicrobial-resistant infections in a Chicago teaching hospital: implications for antibiotic stewardship. Clinical infectious diseases 2009; 49(8): 1175-1184.
McGowan JE. Resistance in nonfermenting gram-negative bacteria: multidrug resistance to the maximum. The American journal of medicine 2006; 119(6): S29-S36.
Magiorakos AP, Srinivasan A, Carey R et al. Multidrug"resistant, extensively drug"resistant and pandrug"resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection 2012; 18(3): 268-281.
Boucher HW, Talbot GH, Bradley JS et al. Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clinical infectious diseases 2009; 48(1): 1-12.
Cosgrove SE, Sakoulas G, Perencevich EN, Schwaber MJ, Karchmer AW, Carmeli Y. Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clinical infectious diseases 2003; 36(1): 53-59.
Walker SS, Labroli M, Painter RE et al. Antibacterial small molecules targeting the conserved TOPRIM domain of DNA gyrase. PloS one 2017; 12(7): e0180965.
Pillar CM, Draghi DC, Sheehan DJ, Sahm DF. Prevalence of multidrug-resistant, methicillin-resistant Staphylococcus aureus in the United States: findings of the stratified analysis of the 2004 to 2005 LEADER Surveillance Programs. Diagnostic microbiology and infectious disease 2008; 60(2): 221-224.
Carey JR, Suslick KS, Hulkower KI et al. Rapid identification of bacteria with a disposable colorimetric sensing array. Journal of the American Chemical Society 2011; 133(19): 7571-7576.
Karami N, Mirzajani F, Rezadoost H et al. Initial study of three different pathogenic microorganisms by gas chromatography-mass spectrometry. F1000Research 2017; 6.
Barker M, Hengst M, Schmid J et al. Volatile organic compounds in the exhaled breath of young patients with cystic fibrosis. Eur Respir J 2006; 27(5): 929 - 936.
Carroll W, Lenney W, Wang T, Å panć›l P, Alcock A, Smith D. Detection of volatile compounds emitted by Pseudomonas aeruginosa using selected ion flow tube mass spectrometry. Pediatric pulmonology 2005; 39(5): 452-456.
Dolch M, Frey L, Hornuss C et al. Molecular breath-gas analysis by online mass spectrometry in mechanically ventilated patients: a new software-based method of CO2-controlled alveolar gas monitoring. Journal of Breath Research 2008; 2(3): 037010.
Dolch M, Hornuss C, Klocke C et al. Volatile compound profiling for the identification of Gram"negative bacteria by ion"molecule reaction–mass spectrometry. Journal of applied microbiology 2012; 113(5): 1097-1105.
Karami N, Karimi A, Aliahmadi A et al. Identification of bacteria using volatile organic compounds. Cellular and Molecular Biology 2017; 63(2): 112-121.
Jünger M, Vautz W, Kuhns M et al. Ion mobility spectrometry for microbial volatile organic compounds: a new identification tool for human pathogenic bacteria. Applied microbiology and biotechnology 2012; 93(6): 2603-2614.
Bean HD, Zhu J, Hill JE. Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS). 2011/06/08/ 2011; (52): e2664.
Allardyce RA, Langford VS, Hill AL, Murdoch DR. Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS). Journal of Microbiological Methods 2006; 65(2): 361-365.
de Heer K, van der Schee MP, Zwinderman K et al. Electronic nose technology for detection of invasive pulmonary aspergillosis in prolonged chemotherapy-induced neutropenia: a proof-of-principle study. Journal of clinical microbiology 2013; 51(5): 1490-1495.
Nizio K, Perrault K, Troobnikoff A et al. In vitro volatile organic compound profiling using GCí— GC-TOFMS to differentiate bacteria associated with lung infections: a proof-of-concept study. Journal of Breath Research 2016; 10(2): 026008.
Boots AW, Smolinska A, Berkel JJBNv et al. Identification of microorganisms based on headspace analysis of volatile organic compounds by gas chromatography–mass spectrometry. Journal of Breath Research 2014; 8(2): 027106.
Filipiak W, Sponring A, Baur MM et al. Molecular analysis of volatile metabolites released specifically by staphylococcus aureus and pseudomonas aeruginosa. BMC microbiology 2012; 12(1): 113.
Bos LD, Sterk PJ, Schultz MJ. Volatile metabolites of pathogens: a systematic review. PLoS pathogens 2013; 9(5): e1003311.
Bean HD, Dimandja J-MD, Hill JE. Bacterial volatile discovery using solid phase microextraction and comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry. Journal of Chromatography B 2012; 901: 41-46.
Julák J, Stránská E, Rosová V, Geppert H, Å panć›l P, Smith D. Bronchoalveolar lavage examined by solid phase microextraction, gas chromatography–mass spectrometry and selected ion flow tube mass spectrometry. Journal of Microbiological Methods 2006; 65(1): 76-86.
Maddula S, Blank LM, Schmid A, Baumbach JI. Detection of volatile metabolites of Escherichia coli by multi capillary column coupled ion mobility spectrometry. Anal Bioanal Chem 2009; 394(3): 791-800.
Tait E, Hill K, Perry J, Stanforth S, Dean J. Development of a novel method for detection of Clostridium difficile using HS"SPME"GC"MS. Journal of applied microbiology 2014; 116(4): 1010-1019.
Tait E, Perry JD, Stanforth SP, Dean JR. Identification of volatile organic compounds produced by bacteria using HS-SPME-GC–MS. Journal of chromatographic science 2014; 52(4): 363-373.
Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols 2008; 3(2): 163-175.
Schulz S, Fuhlendorff J, Reichenbach H. Identification and synthesis of volatiles released by the myxobacterium Chondromyces crocatus. Tetrahedron 2004; 60(17): 3863-3872.
Filipiak W, Sponring A, Baur MM et al. Characterization of volatile metabolites taken up by or released from Streptococcus pneumoniae and Haemophilus influenzae by using GC-MS. Microbiology 2012; 158(Pt 12): 3044-3053.
Landete J. Plant and mammalian lignans: a review of source, intake, metabolism, intestinal bacteria and health. Food Research International 2012; 46(1): 410-424.
Thorn RMS, Reynolds DM, Greenman J. Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains< i> in vitro. Journal of Microbiological Methods 2011; 84(2): 258-264.
Wilson AD, Baietto M. Advances in electronic-nose technologies developed for biomedical applications. Sensors 2011; 11(1): 1105-1176.
Tenover FC. Mechanisms of antimicrobial resistance in bacteria. The American journal of medicine 2006; 119(6): S3-S10.
Preti G, Thaler E, Hanson CW, Troy M, Eades J, Gelperin A. Volatile compounds characteristic of sinus-related bacteria and infected sinus mucus: analysis by solid-phase microextraction and gas chromatography–mass spectrometry. Journal of Chromatography B 2009; 877(22): 2011-2018.
Storer MK, Hibbard-Melles K, Davis B, Scotter J. Detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (SIFT-MS). Journal of Microbiological Methods 2011; 87(1): 111-113.
Kai M, Haustein M, Molina F, Petri A, Scholz B, Piechulla B. Bacterial volatiles and their action potential. Applied microbiology and biotechnology 2009; 81(6): 1001-1012.
Zhu J, Bean HD, Kuo Y-M, Hill JE. Fast detection of volatile organic compounds from bacterial cultures by secondary electrospray ionization-mass spectrometry. Journal of clinical microbiology 2010; 48(12): 4426-4431.
Shestivska V, Nemec A, Drevinek P, Sovova K, Dryahina K, Spanel P. Quantification of methyl thiocyanate in the headspace of Pseudomonas aeruginosa cultures and in the breath of cystic fibrosis patients by selected ion flow tube mass spectrometry. Rapid Commun Mass Spectrom 2011; 25(17): 2459 - 2467.

References

Anderson DJ, Engemann JJ, Harrell LJ, Carmeli Y, Reller LB, Kaye KS. Predictors of mortality in patients with bloodstream infection due to ceftazidime-resistant Klebsiella pneumoniae. Antimicrobial agents and chemotherapy 2006; 50(5): 1715-1720.

Roberts RR, Hota B, Ahmad I et al. Hospital and societal costs of antimicrobial-resistant infections in a Chicago teaching hospital: implications for antibiotic stewardship. Clinical infectious diseases 2009; 49(8): 1175-1184.

McGowan JE. Resistance in nonfermenting gram-negative bacteria: multidrug resistance to the maximum. The American journal of medicine 2006; 119(6): S29-S36.

Magiorakos AP, Srinivasan A, Carey R et al. Multidrug"resistant, extensively drug"resistant and pandrug"resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection 2012; 18(3): 268-281.

Boucher HW, Talbot GH, Bradley JS et al. Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clinical infectious diseases 2009; 48(1): 1-12.

Cosgrove SE, Sakoulas G, Perencevich EN, Schwaber MJ, Karchmer AW, Carmeli Y. Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clinical infectious diseases 2003; 36(1): 53-59.

Walker SS, Labroli M, Painter RE et al. Antibacterial small molecules targeting the conserved TOPRIM domain of DNA gyrase. PloS one 2017; 12(7): e0180965.

Pillar CM, Draghi DC, Sheehan DJ, Sahm DF. Prevalence of multidrug-resistant, methicillin-resistant Staphylococcus aureus in the United States: findings of the stratified analysis of the 2004 to 2005 LEADER Surveillance Programs. Diagnostic microbiology and infectious disease 2008; 60(2): 221-224.

Carey JR, Suslick KS, Hulkower KI et al. Rapid identification of bacteria with a disposable colorimetric sensing array. Journal of the American Chemical Society 2011; 133(19): 7571-7576.

Karami N, Mirzajani F, Rezadoost H et al. Initial study of three different pathogenic microorganisms by gas chromatography-mass spectrometry. F1000Research 2017; 6.

Barker M, Hengst M, Schmid J et al. Volatile organic compounds in the exhaled breath of young patients with cystic fibrosis. Eur Respir J 2006; 27(5): 929 - 936.

Carroll W, Lenney W, Wang T, Å panć›l P, Alcock A, Smith D. Detection of volatile compounds emitted by Pseudomonas aeruginosa using selected ion flow tube mass spectrometry. Pediatric pulmonology 2005; 39(5): 452-456.

Dolch M, Frey L, Hornuss C et al. Molecular breath-gas analysis by online mass spectrometry in mechanically ventilated patients: a new software-based method of CO2-controlled alveolar gas monitoring. Journal of Breath Research 2008; 2(3): 037010.

Dolch M, Hornuss C, Klocke C et al. Volatile compound profiling for the identification of Gram"negative bacteria by ion"molecule reaction–mass spectrometry. Journal of applied microbiology 2012; 113(5): 1097-1105.

Karami N, Karimi A, Aliahmadi A et al. Identification of bacteria using volatile organic compounds. Cellular and Molecular Biology 2017; 63(2): 112-121.

Jünger M, Vautz W, Kuhns M et al. Ion mobility spectrometry for microbial volatile organic compounds: a new identification tool for human pathogenic bacteria. Applied microbiology and biotechnology 2012; 93(6): 2603-2614.

Bean HD, Zhu J, Hill JE. Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS). 2011/06/08/ 2011; (52): e2664.

Allardyce RA, Langford VS, Hill AL, Murdoch DR. Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS). Journal of Microbiological Methods 2006; 65(2): 361-365.

de Heer K, van der Schee MP, Zwinderman K et al. Electronic nose technology for detection of invasive pulmonary aspergillosis in prolonged chemotherapy-induced neutropenia: a proof-of-principle study. Journal of clinical microbiology 2013; 51(5): 1490-1495.

Nizio K, Perrault K, Troobnikoff A et al. In vitro volatile organic compound profiling using GCí— GC-TOFMS to differentiate bacteria associated with lung infections: a proof-of-concept study. Journal of Breath Research 2016; 10(2): 026008.

Boots AW, Smolinska A, Berkel JJBNv et al. Identification of microorganisms based on headspace analysis of volatile organic compounds by gas chromatography–mass spectrometry. Journal of Breath Research 2014; 8(2): 027106.

Filipiak W, Sponring A, Baur MM et al. Molecular analysis of volatile metabolites released specifically by staphylococcus aureus and pseudomonas aeruginosa. BMC microbiology 2012; 12(1): 113.

Bos LD, Sterk PJ, Schultz MJ. Volatile metabolites of pathogens: a systematic review. PLoS pathogens 2013; 9(5): e1003311.

Bean HD, Dimandja J-MD, Hill JE. Bacterial volatile discovery using solid phase microextraction and comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry. Journal of Chromatography B 2012; 901: 41-46.

Julák J, Stránská E, Rosová V, Geppert H, Å panć›l P, Smith D. Bronchoalveolar lavage examined by solid phase microextraction, gas chromatography–mass spectrometry and selected ion flow tube mass spectrometry. Journal of Microbiological Methods 2006; 65(1): 76-86.

Maddula S, Blank LM, Schmid A, Baumbach JI. Detection of volatile metabolites of Escherichia coli by multi capillary column coupled ion mobility spectrometry. Anal Bioanal Chem 2009; 394(3): 791-800.

Tait E, Hill K, Perry J, Stanforth S, Dean J. Development of a novel method for detection of Clostridium difficile using HS"SPME"GC"MS. Journal of applied microbiology 2014; 116(4): 1010-1019.

Tait E, Perry JD, Stanforth SP, Dean JR. Identification of volatile organic compounds produced by bacteria using HS-SPME-GC–MS. Journal of chromatographic science 2014; 52(4): 363-373.

Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols 2008; 3(2): 163-175.

Schulz S, Fuhlendorff J, Reichenbach H. Identification and synthesis of volatiles released by the myxobacterium Chondromyces crocatus. Tetrahedron 2004; 60(17): 3863-3872.

Filipiak W, Sponring A, Baur MM et al. Characterization of volatile metabolites taken up by or released from Streptococcus pneumoniae and Haemophilus influenzae by using GC-MS. Microbiology 2012; 158(Pt 12): 3044-3053.

Landete J. Plant and mammalian lignans: a review of source, intake, metabolism, intestinal bacteria and health. Food Research International 2012; 46(1): 410-424.

Thorn RMS, Reynolds DM, Greenman J. Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains< i> in vitro. Journal of Microbiological Methods 2011; 84(2): 258-264.

Wilson AD, Baietto M. Advances in electronic-nose technologies developed for biomedical applications. Sensors 2011; 11(1): 1105-1176.

Tenover FC. Mechanisms of antimicrobial resistance in bacteria. The American journal of medicine 2006; 119(6): S3-S10.

Preti G, Thaler E, Hanson CW, Troy M, Eades J, Gelperin A. Volatile compounds characteristic of sinus-related bacteria and infected sinus mucus: analysis by solid-phase microextraction and gas chromatography–mass spectrometry. Journal of Chromatography B 2009; 877(22): 2011-2018.

Storer MK, Hibbard-Melles K, Davis B, Scotter J. Detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (SIFT-MS). Journal of Microbiological Methods 2011; 87(1): 111-113.

Kai M, Haustein M, Molina F, Petri A, Scholz B, Piechulla B. Bacterial volatiles and their action potential. Applied microbiology and biotechnology 2009; 81(6): 1001-1012.

Zhu J, Bean HD, Kuo Y-M, Hill JE. Fast detection of volatile organic compounds from bacterial cultures by secondary electrospray ionization-mass spectrometry. Journal of clinical microbiology 2010; 48(12): 4426-4431.

Shestivska V, Nemec A, Drevinek P, Sovova K, Dryahina K, Spanel P. Quantification of methyl thiocyanate in the headspace of Pseudomonas aeruginosa cultures and in the breath of cystic fibrosis patients by selected ion flow tube mass spectrometry. Rapid Commun Mass Spectrom 2011; 25(17): 2459 - 2467.

Author biographies is not available.