Relation of MPO, MnSOD, NQO1 gene variants in endometrial carcinoma in the line of PCR-RFLP methods

Sibel Bulgurcuoglu Kuran; Elif Sinem Iplik; Bedia Cakmakoglu; Ozlem Timirci Kahraman; Ahmet Cem Iyibozkurt; Arzu Koc; Arzu Ergen; Seda Gulec Yilmaz; Turgay Isbir

doi:10.14715/cmb/2018.64.4.13

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Vol. 64 No. 4: Issue 4

Issue Published : April 2, 2018

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Relation of MPO, MnSOD, NQO1 gene variants in endometrial carcinoma in the line of PCR-RFLP methods

https://doi.org/10.14715/cmb/2018.64.4.13

Sibel Bulgurcuoglu Kuran

Department of Obstetrics and Gynecology, Istanbul Faculty of Medicine, Istanbul University, Istanbul Turkey

Elif Sinem Iplik

Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, Istanbul, Turkey

Bedia Cakmakoglu

Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul Turkey

Ozlem Timirci Kahraman

Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul Turkey

Ahmet Cem Iyibozkurt

Department of Obstetrics and Gynecology, Istanbul Faculty of Medicine, Istanbul University, Istanbul Turkey

Arzu Koc

Department of Obstetrics and Gynecology, Sisli Etfal Training and Research Hospital, Istanbul Turkey

Arzu Ergen

Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul Turkey

Seda Gulec Yilmaz

Department of Molecular Medicine, Institute of Health Sciences, Yeditepe University, Istanbul, Turkey

Turgay Isbir

Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul Turkey

Corresponding Author(s) : Sibel Bulgurcuoglu Kuran

sbulgurcuoglu@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 4: Issue 4
Article Published : March 31, 2018

Abstract

Reactive oxygen species (ROS) have been shown to be responsible for inducing DNA damage leading to mutagenesis, carcinogenesis, and cell death if the capacity of the protective antioxidant system is impaired. Endometrial carcinoma is the primary cancer type in the female genital system. The enhanced cell lipid peroxidation and impaired antioxidant enzyme activities observed in patients with endometrial cancer indicate the potential for oxidative injury to cells and cell membranes in such patients. The aim of the study was to investigate the possible association between gene variants of superoxide dismutase (SOD), myeloperoxidase (MPO), and NADPH quinone oxido reductase (NQO1), and their possible role in endometrial cancer in Turkish patients. According to results, MPO G+ genotype and AG genotype were significantly increased in patients compared with controls (P<0.001). We suggest that the MPO polymorphism might be a risk for endometrial cancer.

Keywords

MnSOD NQO1 MPO Polymorphism Endometrial carcinoma.

Bulgurcuoglu Kuran, S., Iplik, E. S., Cakmakoglu, B., Timirci Kahraman, O., Iyibozkurt, A. C., Koc, A., Ergen, A., Gulec Yilmaz, S., & Isbir, T. (2018). Relation of MPO, MnSOD, NQO1 gene variants in endometrial carcinoma in the line of PCR-RFLP methods. Cellular and Molecular Biology, 64(4), 78–82. https://doi.org/10.14715/cmb/2018.64.4.13

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References

Pejic S, Kasapovic J, Todorovic A, Stojiljkovic V and Pajovic SB. Lipid peroxidation and antioxidant status in blood of patients with uterine myoma, endometrial polypus, hyperplastic and malignant endometrium. Biol Res 2006;39: 619-629.
Corocleanu M. Hypothesis for endometrial carcinoma carcinogenesis preventive prospects. Clin Exp Obstet Gynecol 1993;20:254-258.
Cincin ZB, Iyibozkurt AC, Kuran SB, Cakmakoglu B: DNA repair gene variants in endometrial carcinoma. Med Oncol 2012;29(4):2949-2954.
Nicholson MR, Iyengar P, Hummer AJ, Linkov I, Asher M, Soslow RA: Immunophenotypic diversity of endometrial adenocarcinomas: implications for differential diagnosis. Modern Pathol 2009;19:1091-1100.
He C, Tamimi RM, Hankinson SE, Hunter DJ, Han J. A prospective study of genetic polymorphism in MPO, antioxidant status, and breast cancer risk. Breast Cancer Res Treat 2009;113:585–594.
Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16(3):1215.
Hu JJ, Smith TR, Miller MS, Mohrenweiser HW, Golden A, Case LD. Amino acid substitution variants of APE1 and XRCC1 genes associated with ionizing radiation sensitivity. Carcinogenesis 2001;22(6):917–922.
Le Marchand L, Donlon T, Lum-Jones A, Seifried A, Wilkens LR. Association of the HOGG1 Ser326Cys polymorphism with lung cancer risk. Cancer Epidemiol Biomarkers Prev 2002;11(4):409–412.
Tanaguchi N, Ishikawa M, Kawaguchi T, Fujii J, Suzuki K, Nakata T. Expression of Mn-superoxide dismutase in carcinogenesis. Tohoku J Exp Med 1992;168:105– 111.
Arseniy EY, Kutikhin AG: Common Genetic Variants in the Myeloperoxidase and Paraoxonase Genes and the Related Cancer Risk: A Review Part C: Environmental Carcinogenesis and Ecotoxicology Reviews 2012;30: 287-322.
Zappa F, Ward T, Butler J, Pedrinis E, McGown A. Overexpression of NAD(P)H:quinone oxidoreductase 1 in human reproductive system. J Histochem Cytochem 2001;49:1187– 1188.
Rosenblum JS, Gilula NB, Lerner RA. On signal sequence polymorphisms and diseases of distribution. Proc Natl Acad Sci USA 1996;93(9):4471-4473.
Hiroi S, Harada H, Nishi H, Satoh M, Nagai R, Kimura A. Polymorphisms in the SOD2 and HLA-DRB1 genes are associated with nonfamilial idiopathic dilated cardiomyopathy in Japanese. Biochem Biophys Res Commun 1999;261(2):332-339.
Ambrosone CB, Freudenheim JL, Thompson PA, Bowman E, Vena JE, Marshall JR, Graham S, Laughlin R, Nemoto T, Shields PG.: Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer. Cancer Res 1999;59(3):602-606.
Mitrunen K, Sillanpää P, Kataja V, Eskelinen M, Kosma VM, Benhamou S, Uusitupa M, Hirvonen A.: Association between manganese superoxide dismutase (MnSOD) gene polymorphism and breast cancer risk. Carcinogenesis 2001;22(5):827-829.
Woodson K, Tangrea JA, Lehman TA, Modali R, Taylor KM, Snyder K, Taylor PR, Virtamo J, Albanes D.: Manganese superoxide dismutase (MnSOD) polymorphism, alpha-tocopherol supplementation and prostate cancer risk in the alpha-tocopherol, beta-carotene cancer prevention study (Finland). Cancer Causes Control 2003;14(6):513-518.
Wang LI, Miller DP, Sai Y, Liu G, Su L, Wain JC, Lynch TJ, Christiani DC. Manganese superoxide dismutase alanine-to-valine polymorphism at codon 16 and lung cancer risk. J Natl Cancer Inst 2001;93(23):1818-1821.
Park SJ, Zhao H, Spitz MR, Grossman HB, Wu X. An association between NQO1 genetic polymorphism and risk of bladder cancer Mutat Res 2003;536(1-2):131-137.
Schulz WA, Krummeck A, Rösinger I, Eickelmann P, Neuhaus C, Ebert T, Schmitz-Dräger BJ, Sies H. Increased frequency of a null-allele for NAD(P)H: quinone oxidoreductase in patients with urological malignancies. Pharmacogenetics 1997;7(3):235-239.
Hung RJ, Boffetta P, Brennan P, Malaveille C, Gelatti U, Placidi D, Carta A, Hautefeuille A, Porru S.: Genetic polymorphisms of MPO, COMT, MnSOD, NQO1, interactions with environmental exposures and bladder cancer risk. Carcinogenesis 2004;(6): 973-978.
Purdie, D. et al. (2002) Proceedings of the American Association for Cancer Research.
Hou L, Chatterjee N, Huang WY, Baccarelli A, Yadavalli S, Yeager M, Bresalier RS, Chanock SJ, Caporaso NE, Ji BT, Weissfeld JL, Hayes RB. CYP1A1 Val462 and NQO1 Ser187 polymorphisms, cigarette use, and risk for colorectal adenoma. Carcinogenesis 2005;6:1122-1128.
Kiyohara C, Yoshimasu K, Takayama K, Nakanishi Y.: NQO1, MPO, and the risk of lung cancer: a HuGE review. Genet Med 2005;7(7): 463-478.
Hung RJ, Boffetta P, Brennan P, Malaveille C, Gelatti U, Placidi D, Carta A, Hautefeuille A, Porru S .Genetic polymorphisms of MPO, COMT, MnSOD, NQO1, interactions with environmental exposures and bladder cancer risk. Carcinogenesis 2004;25(6):973-978.
Olson SH, Carlson MD, Ostrer H, Harlap S, Stone A, Winters M, Ambrosone CB. Genetic variants in SOD2, MPO, and NQO1, and risk of ovarian cancer. Gynecol Oncol 2004;93(3): 615-620.
Pabalan N, Jarjanazi H, Sung L, Li H, Ozcelik H. Menopausal status modifies breast cancer risk associated with the myeloperoxidase (MPO) G463A polymorphism in Caucasian women: a meta-analysis. PLoS ONE 7:e32389. doi:10.1371/ journal.pone.0032389, 2012.
Qin X, Deng Y, Zeng ZY, Peng QL, Huang XL, Mo CJ, Li S, Zhao JM. Myeloperoxidase polymorphism, menopausal status, and breast cancer risk: an update meta-analysis. PLoS ONE 8:e72583. doi:10.1371/journal.pone.0072583, 2013.
Castelao C, Pereira A, Andreia D, Angela M, Manuel I, Bicho Rui, Maria M, Bicho C. Association of myeloperoxidase polymorphism (G463A) with cervix cancer Mol Cell Biochem 2015;404:1–4.
Kabutomori O, Yanagihara T, Iwatani Y, Kawarazaki A, Kabutomori M. Sex difference in myeloperoxidase activity of neutrophils. Am. J. Hematol 1999;60: 312–313.
Jansson G. Oestrogen-induced enhancement of myeloperoxidase activity in human polymorphonuclear leukocytes”a possible cause of oxidative stress in inflammatory cells. Free Radic. Res. Commun 1991;14: 195–208.
Zuckerman SH, Bryan N: Inhibition of LDL oxidation and myeloperoxidase dependent tyrosyl radical formation by the selective estrogen receptor modulator raloxifene (LY139481 HCL). Atherosclerosis 1996;126: 65–75.
Schabath MB, Spitz MR, Delclos GL, Gunn GB, Whitehead LW, Wu X. Association between asbestos exposure, cigarette smoking, myeloperoxidase (MPO) genotypes, and lung cancer risk Am. J. Ind. Med 2002;42:29–37.
Bauer G: Tumor cell-protective catalase as a novel target for rational therapeutic approaches based on specific intercellular ROS signaling. Anticancer Res 2012;32:2599–2624.
Herdener M, Heigold S, Saran M, Bauer G. Target cellderived superoxide anions cause efficiency and selectivity of intercellular induction of apoptosis. Free Radic Biol Med 2000;29: 1260–1271.
Roy D, Cai Q, Felty Q, Narayan S: Estrogen-induced generation of reactive oxygen and nitrogen species, gene damage, and estrogen-dependent cancers. J Toxicol Environ Health B Crit Rev 2007;10(4):235–257.
Macmahon B. Risk factors for endometrial cancer. Gynecologic Oncology1974; 2, 122.
Modugno F, Ness RB, Chen C, Weiss NS: Inflammation and endometrial cancer: a hypothesis. Cancer epidemiology, biomarkers & prevention 2005;14: 2840-2847.

References

Pejic S, Kasapovic J, Todorovic A, Stojiljkovic V and Pajovic SB. Lipid peroxidation and antioxidant status in blood of patients with uterine myoma, endometrial polypus, hyperplastic and malignant endometrium. Biol Res 2006;39: 619-629.

Corocleanu M. Hypothesis for endometrial carcinoma carcinogenesis preventive prospects. Clin Exp Obstet Gynecol 1993;20:254-258.

Cincin ZB, Iyibozkurt AC, Kuran SB, Cakmakoglu B: DNA repair gene variants in endometrial carcinoma. Med Oncol 2012;29(4):2949-2954.

Nicholson MR, Iyengar P, Hummer AJ, Linkov I, Asher M, Soslow RA: Immunophenotypic diversity of endometrial adenocarcinomas: implications for differential diagnosis. Modern Pathol 2009;19:1091-1100.

He C, Tamimi RM, Hankinson SE, Hunter DJ, Han J. A prospective study of genetic polymorphism in MPO, antioxidant status, and breast cancer risk. Breast Cancer Res Treat 2009;113:585–594.

Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16(3):1215.

Hu JJ, Smith TR, Miller MS, Mohrenweiser HW, Golden A, Case LD. Amino acid substitution variants of APE1 and XRCC1 genes associated with ionizing radiation sensitivity. Carcinogenesis 2001;22(6):917–922.

Le Marchand L, Donlon T, Lum-Jones A, Seifried A, Wilkens LR. Association of the HOGG1 Ser326Cys polymorphism with lung cancer risk. Cancer Epidemiol Biomarkers Prev 2002;11(4):409–412.

Tanaguchi N, Ishikawa M, Kawaguchi T, Fujii J, Suzuki K, Nakata T. Expression of Mn-superoxide dismutase in carcinogenesis. Tohoku J Exp Med 1992;168:105– 111.

Arseniy EY, Kutikhin AG: Common Genetic Variants in the Myeloperoxidase and Paraoxonase Genes and the Related Cancer Risk: A Review Part C: Environmental Carcinogenesis and Ecotoxicology Reviews 2012;30: 287-322.

Zappa F, Ward T, Butler J, Pedrinis E, McGown A. Overexpression of NAD(P)H:quinone oxidoreductase 1 in human reproductive system. J Histochem Cytochem 2001;49:1187– 1188.

Rosenblum JS, Gilula NB, Lerner RA. On signal sequence polymorphisms and diseases of distribution. Proc Natl Acad Sci USA 1996;93(9):4471-4473.

Hiroi S, Harada H, Nishi H, Satoh M, Nagai R, Kimura A. Polymorphisms in the SOD2 and HLA-DRB1 genes are associated with nonfamilial idiopathic dilated cardiomyopathy in Japanese. Biochem Biophys Res Commun 1999;261(2):332-339.

Ambrosone CB, Freudenheim JL, Thompson PA, Bowman E, Vena JE, Marshall JR, Graham S, Laughlin R, Nemoto T, Shields PG.: Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer. Cancer Res 1999;59(3):602-606.

Mitrunen K, Sillanpää P, Kataja V, Eskelinen M, Kosma VM, Benhamou S, Uusitupa M, Hirvonen A.: Association between manganese superoxide dismutase (MnSOD) gene polymorphism and breast cancer risk. Carcinogenesis 2001;22(5):827-829.

Woodson K, Tangrea JA, Lehman TA, Modali R, Taylor KM, Snyder K, Taylor PR, Virtamo J, Albanes D.: Manganese superoxide dismutase (MnSOD) polymorphism, alpha-tocopherol supplementation and prostate cancer risk in the alpha-tocopherol, beta-carotene cancer prevention study (Finland). Cancer Causes Control 2003;14(6):513-518.

Wang LI, Miller DP, Sai Y, Liu G, Su L, Wain JC, Lynch TJ, Christiani DC. Manganese superoxide dismutase alanine-to-valine polymorphism at codon 16 and lung cancer risk. J Natl Cancer Inst 2001;93(23):1818-1821.

Park SJ, Zhao H, Spitz MR, Grossman HB, Wu X. An association between NQO1 genetic polymorphism and risk of bladder cancer Mutat Res 2003;536(1-2):131-137.

Schulz WA, Krummeck A, Rösinger I, Eickelmann P, Neuhaus C, Ebert T, Schmitz-Dräger BJ, Sies H. Increased frequency of a null-allele for NAD(P)H: quinone oxidoreductase in patients with urological malignancies. Pharmacogenetics 1997;7(3):235-239.

Hung RJ, Boffetta P, Brennan P, Malaveille C, Gelatti U, Placidi D, Carta A, Hautefeuille A, Porru S.: Genetic polymorphisms of MPO, COMT, MnSOD, NQO1, interactions with environmental exposures and bladder cancer risk. Carcinogenesis 2004;(6): 973-978.

Purdie, D. et al. (2002) Proceedings of the American Association for Cancer Research.

Hou L, Chatterjee N, Huang WY, Baccarelli A, Yadavalli S, Yeager M, Bresalier RS, Chanock SJ, Caporaso NE, Ji BT, Weissfeld JL, Hayes RB. CYP1A1 Val462 and NQO1 Ser187 polymorphisms, cigarette use, and risk for colorectal adenoma. Carcinogenesis 2005;6:1122-1128.

Kiyohara C, Yoshimasu K, Takayama K, Nakanishi Y.: NQO1, MPO, and the risk of lung cancer: a HuGE review. Genet Med 2005;7(7): 463-478.

Hung RJ, Boffetta P, Brennan P, Malaveille C, Gelatti U, Placidi D, Carta A, Hautefeuille A, Porru S .Genetic polymorphisms of MPO, COMT, MnSOD, NQO1, interactions with environmental exposures and bladder cancer risk. Carcinogenesis 2004;25(6):973-978.

Olson SH, Carlson MD, Ostrer H, Harlap S, Stone A, Winters M, Ambrosone CB. Genetic variants in SOD2, MPO, and NQO1, and risk of ovarian cancer. Gynecol Oncol 2004;93(3): 615-620.

Pabalan N, Jarjanazi H, Sung L, Li H, Ozcelik H. Menopausal status modifies breast cancer risk associated with the myeloperoxidase (MPO) G463A polymorphism in Caucasian women: a meta-analysis. PLoS ONE 7:e32389. doi:10.1371/ journal.pone.0032389, 2012.

Qin X, Deng Y, Zeng ZY, Peng QL, Huang XL, Mo CJ, Li S, Zhao JM. Myeloperoxidase polymorphism, menopausal status, and breast cancer risk: an update meta-analysis. PLoS ONE 8:e72583. doi:10.1371/journal.pone.0072583, 2013.

Castelao C, Pereira A, Andreia D, Angela M, Manuel I, Bicho Rui, Maria M, Bicho C. Association of myeloperoxidase polymorphism (G463A) with cervix cancer Mol Cell Biochem 2015;404:1–4.

Kabutomori O, Yanagihara T, Iwatani Y, Kawarazaki A, Kabutomori M. Sex difference in myeloperoxidase activity of neutrophils. Am. J. Hematol 1999;60: 312–313.

Jansson G. Oestrogen-induced enhancement of myeloperoxidase activity in human polymorphonuclear leukocytes”a possible cause of oxidative stress in inflammatory cells. Free Radic. Res. Commun 1991;14: 195–208.

Zuckerman SH, Bryan N: Inhibition of LDL oxidation and myeloperoxidase dependent tyrosyl radical formation by the selective estrogen receptor modulator raloxifene (LY139481 HCL). Atherosclerosis 1996;126: 65–75.

Schabath MB, Spitz MR, Delclos GL, Gunn GB, Whitehead LW, Wu X. Association between asbestos exposure, cigarette smoking, myeloperoxidase (MPO) genotypes, and lung cancer risk Am. J. Ind. Med 2002;42:29–37.

Bauer G: Tumor cell-protective catalase as a novel target for rational therapeutic approaches based on specific intercellular ROS signaling. Anticancer Res 2012;32:2599–2624.

Herdener M, Heigold S, Saran M, Bauer G. Target cellderived superoxide anions cause efficiency and selectivity of intercellular induction of apoptosis. Free Radic Biol Med 2000;29: 1260–1271.

Roy D, Cai Q, Felty Q, Narayan S: Estrogen-induced generation of reactive oxygen and nitrogen species, gene damage, and estrogen-dependent cancers. J Toxicol Environ Health B Crit Rev 2007;10(4):235–257.

Macmahon B. Risk factors for endometrial cancer. Gynecologic Oncology1974; 2, 122.

Modugno F, Ness RB, Chen C, Weiss NS: Inflammation and endometrial cancer: a hypothesis. Cancer epidemiology, biomarkers & prevention 2005;14: 2840-2847.

Author biographies is not available.