Inhibition of the oxidative stress-induced miR-125b protects glucose metabolic disorders of human retinal pigment epithelium (RPE) cells

Gang Liu; Chun-Di Zhang; Jing Wang; Wan-Cheng Jia

doi:10.14715/cmb/2018.64.4.1

Issue

Vol. 64 No. 4: Issue 4

Issue Published : April 2, 2018

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Inhibition of the oxidative stress-induced miR-125b protects glucose metabolic disorders of human retinal pigment epithelium (RPE) cells

https://doi.org/10.14715/cmb/2018.64.4.1

Gang Liu

Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, 201400, China

Chun-Di Zhang

Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, 201400, China

Jing Wang

Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, 201400, China

Wan-Cheng Jia

Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, 201400, China

Corresponding Author(s) : Wan-Cheng Jia

wanchengjial09@sina.com

Cellular and Molecular Biology, Vol. 64 No. 4: Issue 4
Article Published : March 31, 2018

Abstract

The dysfunction of retinal pigment epithelium (RPE) with aging leads to age-related macular degeneration (AMD). Oxidative stress has been demonstrated as one of the causes of retinal pathological conditions. This study was conducted to investigate the mechanism of hydrogen peroxide (H2O2) induced human retinal pigment epithelial (RPE) cell dysfunction. We report miR-125b is induced by H2O2 treatments in RPE cells. In addition, we observed inhibited glucose metabolism under oxidative stress. Overexpression of miR-125b promotes the disorders of cellular glucose metabolism through direct targeting Hexokinase 2 (HK2). Restoration of HK2 in H2O2 treated RPE cells prevents the oxidative stress-suppressed glucose metabolism. Inhibition of the H2O2-induced miR-125b by inhibitor significantly prevented disorders of glucose metabolism. This study will contribute to the development of the miRNA based therapeutic approaches for against the oxidative stress-mediated human AMD.

Keywords

Retinal pigment epithelium miR-125b Age-related macular degeneration Oxidative stress.

Liu, G., Zhang, C.-D., Wang, J., & Jia, W.-C. (2018). Inhibition of the oxidative stress-induced miR-125b protects glucose metabolic disorders of human retinal pigment epithelium (RPE) cells. Cellular and Molecular Biology, 64(4), 1–5. https://doi.org/10.14715/cmb/2018.64.4.1

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References

References
Ratnapriya R, Chew EY. Age-related macular degeneration-clinical review and genetics update. Clin Genet 2013; 84:160-166.
Bhutto I, Lutty G. Understanding age-related macular degeneration (AMD): Relationships between the photoreceptor/retinal pigment epithelium/Bruch's membrane/choriocapillaris complex. Mol Aspects Med 2012; 33:295-317.
Girmens JF, Sahel JA, Marazova K. Dry age-related macular degeneration: A currently unmet clinical need. Intractable Rare Dis Res 2012; 1:103-114.
Ambati J, Fowler BJ. Mechanisms of age-related macular degeneration. Neuron 2012; 75:26-39.
Forest DL, Johnson LV, Clegg DO. Cellular models and therapies for age-related macular degeneration. Dis Model Mech 2015; 8:421-427.
Bowes RC, Farsiu S, Toth CA, Klingeborn M. Dry age-related macular degeneration: Mechanisms, therapeutic targets, and imaging. Invest Ophthalmol Vis Sci 2013; 54:F68-F80.
Ha M, Kim VN. Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol 2014; 15:509-524.
Lin S, Gregory RI. MicroRNA biogenesis pathways in cancer. Nat Rev Cancer 2015; 15:321-333.
Howell JC, Chun E, Farrell AN, Hur EY, Caroti CM, Iuvone PM, et al. Global microRNA expression profiling: Curcumin (diferuloylmethane) alters oxidative stress-responsive microRNAs in human ARPE-19 cells. Mol Vis 2013; 19:544-560.
Wang S, Koster KM, He Y, Zhou Q. MiRNAs as potential therapeutic targets for age-related macular degeneration. Future Med Chem 2012; 4:277-287.
Zhou Q, Gallagher R, Ufret-Vincenty R, Li X, Olson EN, Wang S. Regulation of angiogenesis and choroidal neovascularization by members of microRNA-23~27~24 clusters. Proc Natl Acad Sci U S A 2011; 108:8287-8292.
Kutty RK, Samuel W, Jaworski C, Duncan T, Nagineni CN, Raghavachari N, et al. MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: Increased expression of microRNA-9 by N-(4-hydroxyphenyl)retinamide. Mol Vis 2010; 16:1475-1486.
Mitter SK, Song C, Qi X, Mao H, Rao H, Akin D, et al. Dysregulated autophagy in the RPE is associated with increased susceptibility to oxidative stress and AMD. Autophagy 2014; 10:1989-2005.
Colussi C, Albertini MC, Coppola S, Rovidati S, Galli F, Ghibelli L. H2O2-induced block of glycolysis as an active ADP-ribosylation reaction protecting cells from apoptosis. FASEB J 2000; 14:2266-2276.
Garg TK, Chang JY. Oxidative stress causes ERK phosphorylation and cell death in cultured retinal pigment epithelium: Prevention of cell death by AG126 and 15-deoxy-delta 12, 14-PGJ2. BMC Ophthalmol 2003; 3:5.
Thurman JM, Renner B, Kunchithapautham K, Ferreira VP, Pangburn MK, Ablonczy Z, et al. Oxidative stress renders retinal pigment epithelial cells susceptible to complement-mediated injury. J Biol Chem 2009; 284:16939-16947.
Lunt SY, Vander HM. Aerobic glycolysis: Meeting the metabolic requirements of cell proliferation. Annu Rev Cell Dev Biol 2011; 27:441-464.
Yokosako K, Mimura T, Funatsu H, Noma H, Goto M, Kamei Y, et al. Glycolysis in patients with age-related macular degeneration. Open Ophthalmol J 2014; 8:39-47.
Winkler BS, Arnold MJ, Brassell MA, Puro DG. Energy metabolism in human retinal Muller cells. Invest Ophthalmol Vis Sci 2000; 41:3183-3190.

References

Ratnapriya R, Chew EY. Age-related macular degeneration-clinical review and genetics update. Clin Genet 2013; 84:160-166.

Bhutto I, Lutty G. Understanding age-related macular degeneration (AMD): Relationships between the photoreceptor/retinal pigment epithelium/Bruch's membrane/choriocapillaris complex. Mol Aspects Med 2012; 33:295-317.

Girmens JF, Sahel JA, Marazova K. Dry age-related macular degeneration: A currently unmet clinical need. Intractable Rare Dis Res 2012; 1:103-114.

Ambati J, Fowler BJ. Mechanisms of age-related macular degeneration. Neuron 2012; 75:26-39.

Forest DL, Johnson LV, Clegg DO. Cellular models and therapies for age-related macular degeneration. Dis Model Mech 2015; 8:421-427.

Bowes RC, Farsiu S, Toth CA, Klingeborn M. Dry age-related macular degeneration: Mechanisms, therapeutic targets, and imaging. Invest Ophthalmol Vis Sci 2013; 54:F68-F80.

Ha M, Kim VN. Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol 2014; 15:509-524.

Lin S, Gregory RI. MicroRNA biogenesis pathways in cancer. Nat Rev Cancer 2015; 15:321-333.

Howell JC, Chun E, Farrell AN, Hur EY, Caroti CM, Iuvone PM, et al. Global microRNA expression profiling: Curcumin (diferuloylmethane) alters oxidative stress-responsive microRNAs in human ARPE-19 cells. Mol Vis 2013; 19:544-560.

Wang S, Koster KM, He Y, Zhou Q. MiRNAs as potential therapeutic targets for age-related macular degeneration. Future Med Chem 2012; 4:277-287.

Zhou Q, Gallagher R, Ufret-Vincenty R, Li X, Olson EN, Wang S. Regulation of angiogenesis and choroidal neovascularization by members of microRNA-23~27~24 clusters. Proc Natl Acad Sci U S A 2011; 108:8287-8292.

Kutty RK, Samuel W, Jaworski C, Duncan T, Nagineni CN, Raghavachari N, et al. MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: Increased expression of microRNA-9 by N-(4-hydroxyphenyl)retinamide. Mol Vis 2010; 16:1475-1486.

Mitter SK, Song C, Qi X, Mao H, Rao H, Akin D, et al. Dysregulated autophagy in the RPE is associated with increased susceptibility to oxidative stress and AMD. Autophagy 2014; 10:1989-2005.

Colussi C, Albertini MC, Coppola S, Rovidati S, Galli F, Ghibelli L. H2O2-induced block of glycolysis as an active ADP-ribosylation reaction protecting cells from apoptosis. FASEB J 2000; 14:2266-2276.

Garg TK, Chang JY. Oxidative stress causes ERK phosphorylation and cell death in cultured retinal pigment epithelium: Prevention of cell death by AG126 and 15-deoxy-delta 12, 14-PGJ2. BMC Ophthalmol 2003; 3:5.

Thurman JM, Renner B, Kunchithapautham K, Ferreira VP, Pangburn MK, Ablonczy Z, et al. Oxidative stress renders retinal pigment epithelial cells susceptible to complement-mediated injury. J Biol Chem 2009; 284:16939-16947.

Lunt SY, Vander HM. Aerobic glycolysis: Meeting the metabolic requirements of cell proliferation. Annu Rev Cell Dev Biol 2011; 27:441-464.

Yokosako K, Mimura T, Funatsu H, Noma H, Goto M, Kamei Y, et al. Glycolysis in patients with age-related macular degeneration. Open Ophthalmol J 2014; 8:39-47.

Winkler BS, Arnold MJ, Brassell MA, Puro DG. Energy metabolism in human retinal Muller cells. Invest Ophthalmol Vis Sci 2000; 41:3183-3190.