Modified PLGA nanofibers as a nerve regenerator with Schwann cells

Davood Zolfaghari; Hamid Tebyanian; Reza Sayyad Soufdoost; Askar Emamgholi; Aref Barkhordari; Gholam Reza Herfedoost; Gholam Reza Kaka; Jamal Rashidiani

doi:10.14715/cmb/2018.64.14.11

Issue

Vol. 64 No. 14: Issue 14

Issue Published : December 3, 2018

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Modified PLGA nanofibers as a nerve regenerator with Schwann cells

https://doi.org/10.14715/cmb/2018.64.14.11

Davood Zolfaghari

Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Hamid Tebyanian

Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran

Reza Sayyad Soufdoost

Imam Khomeini Clinic of Dentistry, Tehran, Iran

Askar Emamgholi

Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Aref Barkhordari

Nanobiotechnology Research Centre, Baqiyatallah University of Medical Science, Tehran, Iran

Gholam Reza Herfedoost

Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Gholam Reza Kaka

Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Jamal Rashidiani

Nanobiotechnology Research Centre, Baqiyatallah University of Medical Science, Tehran, Iran

Corresponding Author(s) : Hamid Tebyanian

tebyan.hamid@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 14: Issue 14
Article Published : November 30, 2018

Abstract

Polylactide-co-glycolide acid (PLGA) is known as a biodegradable and biocompatible polymer. This polymer has been highly used in tissue engineering. In this study, the biological behavior of Schwann cells (Rat) was investigated in co-culture with L lysine/gelatine coated PLGA nano-fiber. In this study, PLGA was dissolved in a hexafluoro propanol based solvent and nanofiber prepared by an electronic method. They were coated with gelatin and poly-L-lysine individually. These polymer properties were investigated by Scanning Electron Microscopy (SEM) analysis and contact angle measurement. After extraction of rat Schwann cells, the cells were cultured in three groups of nano-fiber; nano-fiber PLGA, nano-fiber gelatine coated PLGA and nano-fiber poly-L-lysine coated PLGA. Cell death and Cell proliferation were evaluated by Acridine orange staining (living cell with a green nucleus and dead cell with an orange nucleus) and morphology was investigated by SEM in 2, 4 and 6 days. The diameter of electronic nanofiber PLGA was between 270 to 700 nm. Average contact angles of PLGA, PLGA coated with gelatine, coated with poly-L-lysine and PLGA were 40.12, 64.58 and 107.66degrees, respectively. The findings showed a significant reduction of cell proliferation in PLGA nanofiber ( it was important than PLGA without nano-fiber (P <0.05)). But, this amount was increased in nanofiber which coated with poly-L-lysine and gelatine. PLGA nanofiber-poly-L-lysine was more biocompatible than PLGA nanofiber-gelatine and this comparison was done with rat Schwann cells.

Keywords

Nano-fiber Schwann cells Poly-L-lysine Gelatine.

Zolfaghari, D., Tebyanian, H., Soufdoost, R. S., Emamgholi, A., Barkhordari, A., Herfedoost, G. R., Kaka, G. R., & Rashidiani, J. (2018). Modified PLGA nanofibers as a nerve regenerator with Schwann cells. Cellular and Molecular Biology, 64(14), 66–71. https://doi.org/10.14715/cmb/2018.64.14.11

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References

Evans GR. Challenges to nerve regeneration. Paper presented at: Seminars in surgical oncology, 2000.
Schmidt CE, Leach JB. Neural tissue engineering: strategies for repair and regeneration. Annu Rev Biomed Eng. 2003; 5(1): 293-347.
Noble J, Munro CA, Prasad VSSV, Midha R. Analysis of Upper and Lower Extremity Peripheral Nerve Injuries in a Population of Patients with Multiple Injuries. J Trauma Acute Care Surg. 1998; 45(1): 116-122.
Johnson EO, Soucacos PN. Nerve repair: experimental and clinical evaluation of biodegradable artificial nerve guides. Injury. 2008; 39(3): 30-36.
Yang F, Murugan R, Wang S, Ramakrishna S. Electrospinning of nano/micro scale poly (L-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials. 2005; 26(15): 2603-2610.
Kannan RY, Salacinski HJ, Butler PE, Seifalian AM. Artificial nerve conduits in peripheral"nerve repair. Biotechnol Appl Biochem. 2005; 41(3): 193-200.
Yang F, Murugan R, Ramakrishna S, Wang X, Ma Y-X, Wang S. Fabrication of nano-structured porous PLLA scaffold intended for nerve tissue engineering. Biomaterials. 2004; 25(10): 1891-1900.
Muzzarelli RA. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydr Polym. 2009; 76(2): 167-182.
Möllers S, Heschel I, Damink LHO et al. Cytocompatibility of a novel, longitudinally microstructured collagen scaffold intended for nerve tissue repair. Tissue Eng Part A. 2008; 15(3): 461-472.
Babavalian H, latifi AM, Shokrgozar MA, Bonakdar S, Tebyanian H, Shakeri F. Cloning and expression of recombinant human platelet-derived growth factor-BB in Pichia Pink. Cell Mol Biol (Noisy-le-grand). 2016; 62(8): 45-51.
Tebyanian H, Karami A, Motavallian E et al. A Comparative Study of Rat Lung Decellularization by Chemical Detergents for Lung Tissue Engineering. Open Access Maced J Med Sci. 2017; 5(7): 859-865.
Tebyanian H, Karami A, Motavallian E et al. Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization. Cell Mol Biol (Noisy-le-grand). 2017; 63(7): 46-51.
Hashemi Z, Soleimani M. Tissue engineering scaffolds: History, types and construction methods. ASJ. 2011; 9(35): 146-168.
Croll TI, O'Connor AJ, Stevens GW, Cooper-White JJ. Controllable surface modification of poly (lactic-co-glycolic acid)(PLGA) by hydrolysis or aminolysis I: physical, chemical, and theoretical aspects. Biomacromolecules. 2004; 5(2): 463-473.
Kraemer M, Chaudhuri J, Ellis M, De Bank P. Promotion of Neurite Outgrowth via Incorporation of Poly-L-lysine into Aligned PLGA Nanofibre Scaffolds. Eur Cell Mater. 2011; 22(S3): 53.
Yao L, Wang S, Cui W et al. Effect of functionalized micropatterned PLGA on guided neurite growth. Acta Biomaterialia. 2009; 5(2): 580-588.
Nomura H, Tator CH, Shoichet MS. Bioengineered strategies for spinal cord repair. J Neurotrauma. 2006; 23(3-4): 496-507.
Yuan Y, Zhang P, Yang Y, Wang X, Gu X. The interaction of Schwann cells with chitosan membranes and fibers in vitro. Biomaterials. 2004; 25(18): 4273-4278.
Babavalian H, Latifi AM, Shokrgozar MA, Bonakdar S, Shakeri F, Tebyanian H. Healing Effects of Synthetic and Commercial Alginate Hydrogel Dressings on Wounds: A Comparative Study. Trauma Mon. 2017; 22(6): e38941.
Shakeri F, Tebyanian H, Karami A, Babavalian H, Tahmasbi MH. Effect of Topical Phenytoin on Wound Healing. Trauma Mon. 2017; 22(5): e35488.
Vasita R, Katti DS. Nanofibers and their applications in tissue engineering. Int J Nanomedicine. 2006; 1(1): 15.
Cheung H-Y, Lau K-T, Lu T-P, Hui D. A critical review on polymer-based bio-engineered materials for scaffold development. COMPOS PART B ENG. 2007; 38(3): 291-300.
Karami A, Tebyanian H, Goodarzi V, Shiri S. Planarians: an In Vivo Model for Regenerative Medicine. Int J Stem Cells. 2015; 8(2): 128.
Heller J. Development of poly (ortho esters): a historical overview. Biomaterials. 1990; 11(9): 659-665.
Beck LR, Pope VZ. Controlled-release delivery systems for hormones. Drugs. 1984; 27(6): 528-547.
Mazia D, Schatten G, Sale W. Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy. J Cell Biol. 1975; 66(1): 198-200.
De Jong WH, Borm PJ. Drug delivery and nanoparticles: applications and hazards. Int J Nanomedicine. 2008; 3(2): 133.
Agarwal S, Wendorff JH, Greiner A. Use of electrospinning technique for biomedical applications. Polymer. 2008; 49(26): 5603-5621.
Pham QP, Sharma U, Mikos AG. Electrospinning of polymeric nanofibers for tissue engineering applications: a review. "ŽJ Tissue Eng 2006; 12(5): 1197-1211.
Matthews JA, Wnek GE, Simpson DG, Bowlin GL. Electrospinning of collagen nanofibers. Biomacromolecules. 2002; 3(2): 232-238.
Hedayatpour A, Sobhani A, Bayati V, Abdolvahhabi M, Shokrgozar M, Barbarestani M. A method for isolation and cultivation of adult Schwann cells for nerve conduit. Arch Iran Med. Oct 2007; 10(4): 474-480.
Saravanan K, Büssow H, Weiler N, Gieselmann V, Franken S. A spontaneously immortalized Schwann cell line to study the molecular aspects of metachromatic leukodystrophy. J Neurosci Methods. 2007; 161(2): 223-233.
Guest JD, Hesse D, Schnell L, Schwab ME, Bunge MB, Bunge RP. Influence of IN-1 antibody and acidic FGF-fibrin glue on the response of injured corticospinal tract axons to human Schwann cell grafts. J Neurosci Res. 1997; 50(5): 888-905.
Hood B, Levene HB, Levi AD. Transplantation of autologous Schwann cells for the repair of segmental peripheral nerve defects. Neurosurg Focus. 2009/02/01 2009; 26(2): E4.
Matsas R, Lavdas AA, Papastefanaki F, Thomaidou D. Schwann Cell Transplantation for CNS Repair. Curr Med Chem. 2008; 15(2): 151-160.
Calderón-MartıÌnez D, Garavito Z, Spinel C, Hurtado H. Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C). J Neurosci Methods. 2002; 114(1): 1-8.
Verdú E, RodrıÌguez FJ, Gudiño-Cabrera G, Nieto-Sampedro M, Navarro X. Expansion of adult Schwann cells from mouse predegenerated peripheral nerves. J Neurosci Methods. 2000; 99(1–2): 111-117.
Komiyama T, Nakao Y, Toyama Y, Asou H, Vacanti CA, Vacanti MP. A novel technique to isolate adult Schwann cells for an artificial nerve conduit. J Neurosci Methods. 2003; 122(2): 195-200.
Panseri S, Cunha C, Lowery J et al. Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections. BMC Biotechnol. 2008; 8(1): 39.

References

Evans GR. Challenges to nerve regeneration. Paper presented at: Seminars in surgical oncology, 2000.

Schmidt CE, Leach JB. Neural tissue engineering: strategies for repair and regeneration. Annu Rev Biomed Eng. 2003; 5(1): 293-347.

Noble J, Munro CA, Prasad VSSV, Midha R. Analysis of Upper and Lower Extremity Peripheral Nerve Injuries in a Population of Patients with Multiple Injuries. J Trauma Acute Care Surg. 1998; 45(1): 116-122.

Johnson EO, Soucacos PN. Nerve repair: experimental and clinical evaluation of biodegradable artificial nerve guides. Injury. 2008; 39(3): 30-36.

Yang F, Murugan R, Wang S, Ramakrishna S. Electrospinning of nano/micro scale poly (L-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials. 2005; 26(15): 2603-2610.

Kannan RY, Salacinski HJ, Butler PE, Seifalian AM. Artificial nerve conduits in peripheral"nerve repair. Biotechnol Appl Biochem. 2005; 41(3): 193-200.

Yang F, Murugan R, Ramakrishna S, Wang X, Ma Y-X, Wang S. Fabrication of nano-structured porous PLLA scaffold intended for nerve tissue engineering. Biomaterials. 2004; 25(10): 1891-1900.

Muzzarelli RA. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydr Polym. 2009; 76(2): 167-182.

Möllers S, Heschel I, Damink LHO et al. Cytocompatibility of a novel, longitudinally microstructured collagen scaffold intended for nerve tissue repair. Tissue Eng Part A. 2008; 15(3): 461-472.

Babavalian H, latifi AM, Shokrgozar MA, Bonakdar S, Tebyanian H, Shakeri F. Cloning and expression of recombinant human platelet-derived growth factor-BB in Pichia Pink. Cell Mol Biol (Noisy-le-grand). 2016; 62(8): 45-51.

Tebyanian H, Karami A, Motavallian E et al. A Comparative Study of Rat Lung Decellularization by Chemical Detergents for Lung Tissue Engineering. Open Access Maced J Med Sci. 2017; 5(7): 859-865.

Tebyanian H, Karami A, Motavallian E et al. Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization. Cell Mol Biol (Noisy-le-grand). 2017; 63(7): 46-51.

Hashemi Z, Soleimani M. Tissue engineering scaffolds: History, types and construction methods. ASJ. 2011; 9(35): 146-168.

Croll TI, O'Connor AJ, Stevens GW, Cooper-White JJ. Controllable surface modification of poly (lactic-co-glycolic acid)(PLGA) by hydrolysis or aminolysis I: physical, chemical, and theoretical aspects. Biomacromolecules. 2004; 5(2): 463-473.

Kraemer M, Chaudhuri J, Ellis M, De Bank P. Promotion of Neurite Outgrowth via Incorporation of Poly-L-lysine into Aligned PLGA Nanofibre Scaffolds. Eur Cell Mater. 2011; 22(S3): 53.

Yao L, Wang S, Cui W et al. Effect of functionalized micropatterned PLGA on guided neurite growth. Acta Biomaterialia. 2009; 5(2): 580-588.

Nomura H, Tator CH, Shoichet MS. Bioengineered strategies for spinal cord repair. J Neurotrauma. 2006; 23(3-4): 496-507.

Yuan Y, Zhang P, Yang Y, Wang X, Gu X. The interaction of Schwann cells with chitosan membranes and fibers in vitro. Biomaterials. 2004; 25(18): 4273-4278.

Babavalian H, Latifi AM, Shokrgozar MA, Bonakdar S, Shakeri F, Tebyanian H. Healing Effects of Synthetic and Commercial Alginate Hydrogel Dressings on Wounds: A Comparative Study. Trauma Mon. 2017; 22(6): e38941.

Shakeri F, Tebyanian H, Karami A, Babavalian H, Tahmasbi MH. Effect of Topical Phenytoin on Wound Healing. Trauma Mon. 2017; 22(5): e35488.

Vasita R, Katti DS. Nanofibers and their applications in tissue engineering. Int J Nanomedicine. 2006; 1(1): 15.

Cheung H-Y, Lau K-T, Lu T-P, Hui D. A critical review on polymer-based bio-engineered materials for scaffold development. COMPOS PART B ENG. 2007; 38(3): 291-300.

Karami A, Tebyanian H, Goodarzi V, Shiri S. Planarians: an In Vivo Model for Regenerative Medicine. Int J Stem Cells. 2015; 8(2): 128.

Heller J. Development of poly (ortho esters): a historical overview. Biomaterials. 1990; 11(9): 659-665.

Beck LR, Pope VZ. Controlled-release delivery systems for hormones. Drugs. 1984; 27(6): 528-547.

Mazia D, Schatten G, Sale W. Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy. J Cell Biol. 1975; 66(1): 198-200.

De Jong WH, Borm PJ. Drug delivery and nanoparticles: applications and hazards. Int J Nanomedicine. 2008; 3(2): 133.

Agarwal S, Wendorff JH, Greiner A. Use of electrospinning technique for biomedical applications. Polymer. 2008; 49(26): 5603-5621.

Pham QP, Sharma U, Mikos AG. Electrospinning of polymeric nanofibers for tissue engineering applications: a review. "ŽJ Tissue Eng 2006; 12(5): 1197-1211.

Matthews JA, Wnek GE, Simpson DG, Bowlin GL. Electrospinning of collagen nanofibers. Biomacromolecules. 2002; 3(2): 232-238.

Hedayatpour A, Sobhani A, Bayati V, Abdolvahhabi M, Shokrgozar M, Barbarestani M. A method for isolation and cultivation of adult Schwann cells for nerve conduit. Arch Iran Med. Oct 2007; 10(4): 474-480.

Saravanan K, Büssow H, Weiler N, Gieselmann V, Franken S. A spontaneously immortalized Schwann cell line to study the molecular aspects of metachromatic leukodystrophy. J Neurosci Methods. 2007; 161(2): 223-233.

Guest JD, Hesse D, Schnell L, Schwab ME, Bunge MB, Bunge RP. Influence of IN-1 antibody and acidic FGF-fibrin glue on the response of injured corticospinal tract axons to human Schwann cell grafts. J Neurosci Res. 1997; 50(5): 888-905.

Hood B, Levene HB, Levi AD. Transplantation of autologous Schwann cells for the repair of segmental peripheral nerve defects. Neurosurg Focus. 2009/02/01 2009; 26(2): E4.

Matsas R, Lavdas AA, Papastefanaki F, Thomaidou D. Schwann Cell Transplantation for CNS Repair. Curr Med Chem. 2008; 15(2): 151-160.

Calderón-MartıÌnez D, Garavito Z, Spinel C, Hurtado H. Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C). J Neurosci Methods. 2002; 114(1): 1-8.

Verdú E, RodrıÌguez FJ, Gudiño-Cabrera G, Nieto-Sampedro M, Navarro X. Expansion of adult Schwann cells from mouse predegenerated peripheral nerves. J Neurosci Methods. 2000; 99(1–2): 111-117.

Komiyama T, Nakao Y, Toyama Y, Asou H, Vacanti CA, Vacanti MP. A novel technique to isolate adult Schwann cells for an artificial nerve conduit. J Neurosci Methods. 2003; 122(2): 195-200.

Panseri S, Cunha C, Lowery J et al. Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections. BMC Biotechnol. 2008; 8(1): 39.

Author biographies is not available.