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Effects of down-regulated steroidogenic factor-1 on ACTH and potassium chloride-induced steroid synthesis in H295R cells
Corresponding Author(s) : D Hu
hdlhdl2008@163.com
Cellular and Molecular Biology,
Vol. 61 No. 1: Issue 1
Abstract
The prevalence of adrenal diseases in the cortex is more common than that in the medulla in the form of hormone disorder or neoplasm. Steroidogenic factor-1 (SF-1) is important in regulating aldosterone synthase (CYP11B2) and cortisol synthase (CYP11B1). SF-1 is increased in aldosterone-producing adenoma (APA) and cortisol-producing adenoma (CPA). Overexpression of SF-1 has been extensively studied, but the available in-depth information regarding the effects of downregulated SF-1 on CYP11B2/CYP11B1 and their regulators is limited. In this paper, we attempted to investigate the effects of downregulated SF-1 on aldosterone to adrenocorticotropic hormone (ACTH) and potassium chloride (KCl) stimulation and those on cortisol to ACTH stimulation through RNA interference in acute and chronic phases. Downregulated SF-1 decreased the sensitivity of aldosterone to ACTH/KCl and that of cortisol to ACTH stimulation. This study provides new insights into the influence of SF-1 on adrenocortical diseases by considering the effects of SF-1 on regulation.
Keywords
Steroidogenic factor-1
Aldosterone
Cortisol
ACTH
H295R cells.
Hu, D., Wang, X., Cao, R., & Ding, X. (2015). Effects of down-regulated steroidogenic factor-1 on ACTH and potassium chloride-induced steroid synthesis in H295R cells. Cellular and Molecular Biology, 61(1), 51–55. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/637
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