Mula  A; Yan  Bai; Bingcheng  Hu; Yunhai  Su; Xingxing Yuan

doi:10.14715/cmb/2023.69.9.17

Issue

Vol. 69 No. 9: Issue 9

Issue Published : October 8, 2023

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MiR-669b-5p inhibits the Alzheimer's disease development via regulation of CHEK2 in Neuro2a APPSwe/Δ9 cells

MiR-669b-5p inhibits the Alzheimer's development

https://doi.org/10.14715/cmb/2023.69.9.17

Mula A

Department of Encephalopathy, Heilongjiang Academy of Traditional Chinese Medicine, No. 33 of West Dazhi Street, Harbin, Heilongjiang 150001, P.R. China

Yan Bai

Department of Encephalopathy, Heilongjiang Academy of Traditional Chinese Medicine, No. 33 of West Dazhi Street, Harbin, Heilongjiang 150001, P.R. China

Bingcheng Hu

Department of Encephalopathy, Heilongjiang Academy of Traditional Chinese Medicine, No. 33 of West Dazhi Street, Harbin, Heilongjiang 150001, P.R. China

Yunhai Su

Department of Encephalopathy, Heilongjiang Academy of Traditional Chinese Medicine, No. 33 of West Dazhi Street, Harbin, Heilongjiang 150001, P.R. China

Xingxing Yuan

Department of General Medicine, Heilongjiang Academy of Traditional Chinese Medicine, No. 33 of West Dazhi Street, Harbin, Heilongjiang 150001, P.R. China

Corresponding Author(s) : Xingxing Yuan

jackie198711@163.com

Cellular and Molecular Biology, Vol. 69 No. 9: Issue 9
Article Published : September 30, 2023

Abstract

DNA damage of neurons is accumulated in Alzheimer's disease (AD). DNA damage-activated Checkpoint kinase 2 (CHEK2) is evaluated in Aβ-treated Neuro2a APPSwe/Δ9 cells, and the miR-669b-5p was specifically down-regulated. However, the underlying molecular mechanism between CHEK2 and miR-669b-5p in Neuro2a APPSwe/Δ9 cells remains unclear. This research discovers that in A-treated Neuro2a APPSwe/Δ9 cells, CHEK2 expression and miR-669b-5p expression were inversely correlated. In addition, miR-669b-5p mimics increased cell survival and proliferation in Neuro2a APPSwe/Δ9 cells while decreasing the production of inflammatory cytokines and cell death. Furthermore, it is observed that CHEK2 was a miR-669b-5p downstream target gene and that CHEK2 restored the miR-669b-5p's functions. According to this research, miR-669b-5p is a potential therapy for Alzheimer's patients since it slows the advancement of the disease.

Keywords

miR-669b-5p CHEK2 Aβ Alzheimer Neuro2a APPSwe/Δ9 cells

A, M. ., Bai, Y. ., Hu, B. ., Su, Y. ., & Yuan, X. (2023). MiR-669b-5p inhibits the Alzheimer’s disease development via regulation of CHEK2 in Neuro2a APPSwe/Δ9 cells: MiR-669b-5p inhibits the Alzheimer’s development. Cellular and Molecular Biology, 69(9), 118–124. https://doi.org/10.14715/cmb/2023.69.9.17