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Vol. 66 No. 8: Issue 8

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Resistance to bacterial infection, complication occurring after cardiac surgery

https://doi.org/10.14715/cmb/2020.66.8.4
Payman Akram Hamasaeed
Biology Department, Education College, Salahaddin University, Erbil, Iraq

Corresponding Author(s) : Payman Akram Hamasaeed

payman.hamasaeed@su.edu.krd

Cellular and Molecular Biology, Vol. 66 No. 8: Issue 8

Abstract

To analyze the occurrence of resistant bacterial infection in patients undergoing cardiac surgery hospitalized in the surgical specialty hospital, in Erbil city, Iraq. A prospective study was done on a total of 138 patients operated and hospitalized in an intensive care unit and surgical wards. Bacterial isolates identification was done according to cultural characteristics, microscopic examination, some biochemical tests, analytic Profile Index 20E& API Staph, confirmed with VITEK® 2 compact system (BioMérieux). Antimicrobial susceptibility for disc diffusion tested to 17 antimicrobial agents. Resistance isolates were confirmed phenotypically for carbapenemase by Rapidec Carba NP Test (bioMe´rieux SA, Marcy-l'E´toile, France) for ESBLs producers by ESBL screening test VITEK 2 system. Molecularly blaIMP blaTEM, blaKPC, AmpC and blaCTX-M were detected by PCR. In 134 patients, 28.3% of patients got infected post-operatively. The most frequent source of isolation was from ICU patients (75%). Isolated bacteria included gram-positive 29 (54.7%) and gram-negative bacteria 24 (45.3%). Most frequently:  Staphylococcus aureus (24.4%), each of pseudomonas aeroginosa, Klebsiella pneumonia (15.1%), Streptococcus spp. (11.3%), Escherichia coli (9.4%). Whereas included Coagulase Negative Staphylococci species (CoNS) (13.2%) and Enterococci species (5.7) Statistical analysis showed significantly higher sensitive isolates as compared with resistance isolates. Resistance to Carbapenems calss was 18.9% and Cephalosporins class 41.5% of isolates. The antimicrobial resistance pattern indicated that MDR bacterial isolates (81.1%) were widespread. Of the 34 phenotypically ESBL positive isolates, the ESBL genes (AmpC, blaCTX-M, and blaTEM) were amplified in 7(20.6), 6(17.6) and 6(17.6) isolates respectively. Out of 8 K. pneumonia (37.5%) harboring both blaAmpC and bla-CTX-M genes, while 6(75%) carries blaTEM. The blaCTX-M gene was found in only 1 (12.5%) out of 8 isolates of P. aeruginosa. While blaAmpC genotyping revealed that 1(7.7%) out of 13 Staph. aureus isolates were harboring it. Finally, 3(60%) out of 5 E. coli isolates harboring both AmpC and bla-CTX-M genes. Cardiac surgery patients wound show increasingly emerging strains of ESBL-producing gram-negative bacteria K. pneumonia, P. aeruginosa and E. coli especially patients prolonged in the intensive care unit.

Keywords

Cardiac Surgery Bacterial Infection Resistance genes ICU MDR.
Hamasaeed, P. A. (2020). Resistance to bacterial infection, complication occurring after cardiac surgery. Cellular and Molecular Biology, 66(8), 20–25. https://doi.org/10.14715/cmb/2020.66.8.4
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References
  1. Lepelletier DC, Bourigault JC, Roussel et al. Epidemiology and prevention of surgical site infections after cardiac surgery. Med Mal Infect 2013; 43: 403-409
  2. Livermoore DM, Paterson DL. Pocket guide to extended-spectrum β-lactamases in resistance. J Antimicrob Chemother 2006; 58:231-237.
  3. Nordmann P. Carbapenemase-producing Enterobacteriaceae: overview of a major public health challenge. Med Mal Infec 2014; 44: 51-56.
  4. Muller A et al. Higher third-generation cephalosporin prescription proportion is associated with lower probability of reducing carbapenem use: a nationwide retrospective study. Antimic Resist Infect Cont 2018; 7: 1-10.
  5. Kelly M, Senthil DK, Sinha OR, Vinodh Kumar, Ajay Kumar Yadav BS, Pruthvishree Prasanna Vadhana KR, Nirupama Monika Bhardwaj and Singh BR. Epidemiology of carbapenem-resistant Escherichia coli and first report of blaVIM carbapenemases gene in calves from India. Epidemiol Infect 2019; 147: e159.
  6. Finegold S, M and Baron EJ. Baily and Scotts DiagnosticMicrobiology. Eight Ed. The C.V Mosby Company. 1998 USA.
  7. Clinical and Laboratory Standards Institute. Performance Standards or Antimicrobial Susceptibility Testing: 29th Informational Supplement. 2019; M100"‘S29. Wayne, PA: Clinical and Laboratory Standards Institute.
  8. Nordmann P, Poirel L, Dortet L. Rapid detection of carbapenemase"‘producing Enterobacteriaceae. Emerg Infect Dis 2012;18:1503-1507.
  9. Cristina Quenia, Gonçalves da Silva, Maria Helena Barbos. Risk factors for surgical site infection in cardiac surgery Acta Paul. Enferm 2012; 25.
  10. Lemaignen A, Birgand G, Ghodhbane W, et al. Sternal wound infection after cardiac surgery: incidence and risk factors according to clinical presentation. Clin Microbiol Infect 2015; 21(7):674.e11-674.e6.74E18.
  11. Lubega A, Joel B, Justina Lucy N. Incidence and Etiology of Surgical Site Infections among Emergency Postoperative Patients in Mbarara Regional Referral Hospital, South Western Uganda. Surg Res Pract 2017; 2017: 6365172.
  12. Bustamante-Munguira Juan , Francisco Herrera-Gómez, Miguel Ruiz-ílvarez, Ana Hernández-Aceituno, and Angels Figuerola-Tejerina 2019.A New Surgical Site Infection Risk Score: Infection Risk Index in Cardiac Surgery J Clin Med 2019; 8(4): 480.
  13. Al-Charrakh AH, Samira Y, Yousif Hussein S. Al-Janabi. Occurrence and detection of extended-spectrum - lactamases in Klebsiella isolates in Hilla, Iraq African J Biotech 2020; 10 (4): 657-665
  14. Al-Hasnawy HH, Rashaa Hadi Saleh, Bara´ Hamid Hadi. Existence of ESBL genes in Escherichia coli and Acinetobacter baumannii isolated from different clinical specimens. Pharm Sci Res 2018; 10(5): 1112-1117.
  15. Nasser Mahfouz, Samarpita Gayena, Arun S, Kharatab. Prevalence of β-lactamase and antibiotic-resistant Pseudomonas aeruginosa in the Arab region. J Glob Antimicrob Resistance 2020; 22:152-160
  16. Hussein Z, Kadhim H, Hassan J. Detection of New Delhi Metallo"‘Beta"‘Lactamase"‘1 (blaNDM"‘1) in Carbapenem"‘Resistant Pseudomonas aeruginosa Isolated from clinical samples in Wasit hospitals. Iraqi JMS 2018; 16:239"‘46.
  17. Al"‘Abedi KH, Al"‘Mayahi FA. Molecular detection of serine carbapenemase genes in carbapenem"‘resistant isolates of Pseudomonas aeruginosa recovered from patients in Al"‘Diwaniyah province, Iraq. J Pure Appl Microbiol 2019; 13:1775"‘1782.
  18. Jarallah EM, Alammary MJ. Nosocomial outbreak of carbapenem"‘resistant Pseudomonas aeruginosa producing metallo beta lactamase in Al"‘Hilla teaching hospital, Babylon. J Babylon Univer 2012; 22:317"‘23.
  19. Shilba AA, Al"‘Azzawi RH. Al"‘Awadi SJ. Dissemination of carbapenem resistant Pseudomonas aeruginosa among burn patients in Karbala province Iraq. Iraqi J Sci 2015; 56:1850"‘1857.
  20. Al"‘Charrakh AH, Al"‘Awadi SJ, Mohammed AS. Detection of metallo"‘β"‘lactamase producing Pseudomonas aeruginosa isolated from public and private hospitals in Baghdad, Iraq. Acta Med Iran 2016; 54:107"‘113.
  21. Al"‘Khudhairy MK, Al"‘Shammari MM. Prevalence of metallo"‘β"‘lactamase"‘producing Pseudomonas aeruginosa isolated from diabetic foot infections in Iraq. New Microbes New Infect 2020; 35:1"‘6.
  22. Yassin NA, Khalid HM, Hassan AO. Prevalence of metallo"‘β"‘lactamase producing Pseudomonas aeruginosa in wound infections in Duhok City, Iraq. Int J Res Med Sci 2014; 2:1576"‘1579.
  23. Chiu S, Wu T, Chuang Y, Lin J, Fung C, Lu P, Wang J, Wang L, Siu L and Yeh K. National surveillance study on carbapenem non-susceptible K.pneumoniae in Taiwan: the emergence and rapid dissemination of KPC-2 carbapenemase. PLOS ONE 2013; 7: p:e69428.
  24. Helmy MM and Wasf R. Phenotypic and Molecular Characterization of Plasmid Mediated AmpC B-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospital. Bio Med Res Inter 2014; p:8.
  25. Shacheraghi F, Shakibaie MR, Noveiri H. Molecular identification of ESBL Genes blaGES-blaVEB-blaCTX-M blaOXAblaOXA-4, blaOXA-10 andblaPER-in Pseudomonas aeruginosa strains isolated from burn patients by PCR, RFLP and sequencing techniques. Int J Biol Life Sci 2010; 3(6): 138-142
  26. Zhao WH. & Hu ZQ. Epidemiology and genetics of CTX-M extended-spectrum β-lactamases in Gram-negative bacteria. Crit Rev Microbio 2013; 39: 79-101.
  27. Sydnor ER, Perl TM. Hospital epidemiology and infection control in acute-care settings. Clin Microbiol Rev 2011; 24: 141–173.
  28. Armand-Lefevre L, Angebault C, Barbier F, Hamelet E, Defrance G, Ruppé E, Bronchard R, Lepeule R, Lucet JC, El Mniai A. Emergence of imipenem-resistant Gram-negative bacilli in intestinal flora of intensive care patients. Antimicrob Agents Chemother 2013; 57:1488–1495.
  29. Braun SD, Stefan M, Alexander Th, Antje Rt, Oliwia M, Mathias P, Annett R, Peter S, and Ralf E. Rapid Identification of Carbapenemase Genes in Gram-Negative Bacteria with an Oligonucleotide Microarray-Based Assay. PLOS ONE 2014; 9(7): e102232
  30. Theuretzbacher U. Antibiotic innovation for future public health needs. Clin Microbiol Infec 2017; 23: 713-717.
  31. Lin J, Wang Y, Wei X, Kong S, Liu Z, Liu J, Zhang F, Lin S, Ji B, Zhou Z, Guo Z. Controllable antibacterial and bacterially anti-adhesive surface fabricated by a bio-inspired beetle-like macromolecule. Int J Biol Macromol 2020. Doi: 10.1016/j.ijbiomac.2020.04.207.
  32. Su Q, Liu Y, Lv XW, Dai RX, Yang XH, Kong BH. LncRNA TUG1 mediates ischemic myocardial injury by targeting miR-132-3p/HDAC3 axis. Am J Physiol Heart Circ Physiol 2020; 318(2):H332-44. Doi: 10.1152/ajpheart.00444.2019.
  33. Liu G, Ren G, Zhao L, Cheng L, Wang C, Sun B. Antibacterial activity and mechanism of bifidocin A against Listeria monocytogenes. Food Control 2017; 73:854-61. Doi: 10.1016/j.foodcont.2016.09.036.
  34. Jiang D, Chen FX, Zhou H, Lu YY, Tan H, Yu SJ, Yuan J, Liu H, Meng W, Jin ZB. Bioenergetic crosstalk between mesenchymal stem cells and various ocular cells through the intercellular trafficking of mitochondria. Theranostics 2020;10(16):7260. Doi: 10.7150/thno.46332.
  35. Pan D, Xia XX, Zhou H, Jin SQ, Lu YY, Liu H, Gao ML, Jin ZB. COCO enhances the efficiency of photoreceptor precursor differentiation in early human embryonic stem cell-derived retinal organoids. Stem Cell Res Ther 2020; 11(1):1-2. Doi: 10.1186/s13287-020-01883-5.
  36. Zhang J, Liu B. A review on the recent developments of sequence-based protein feature extraction methods. Curr Bioinform 2019; 14(3):190-9. Doi: 10.2174/1574893614666181212102749.
  37. Xu L, Jiang S, Zou Q. An in silico approach to identification, categorization and prediction of nucleic acid binding proteins. bioRxiv. 2020. Doi: 10.1093/bib/ bbaa171.
  38. Zhu S, Wang X, Zheng Z, Zhao XE, Bai Y, Liu H. Synchronous measuring of triptolide changes in rat brain and blood and its application to a comparative pharmacokinetic study in normal and Alzheimer's disease rats. Journal of Pharmaceutical and Biomedical Analysis. 2020; 113263. 10.1016/j.jpba.2020.113263.
  39. Chen G, Li Y, Ren Z, Gu Y, Tang F, Mao J, Zhu J, Wang L, Li Y. Clinical Significance of MicroRNA-155-Regulated Autophagy and Apoptosis by Targeting Rictor/Fos in Gastric Cancer Progression. Nanosci Nanotechnol Lett 2020; 12(4):525-35.
  40. Alkhudhayri AA, Wahab R, Siddiqui MA, Ahmad J. Selenium Nanoparticles Induce Cytotoxicity and Apoptosis in Human Breast Cancer (MCF-7) and Liver (HepG2) Cell Lines. Nanosci Nanotechnol Lett 2020; 12(3):324-30.
  41. Jin Y, Zhu H, Zhu H, Jin F, Shi C, Yang L, Qian J, Zhang S. Fluorouracil Nanoliposomes Promote Apoptosis of Human Gastric Cancer Xenografts in Nude Mice. Nanosci. Nanotechnol. Lett 2020; 12: 690–695.
  42. Zhang T, Su H, Xing Y, Zhang J, Xu D. Protective Mechanism of Lipid-Lowering Ketone and Self-Assembled OA Chitosan Nanoparticles on Insulin Oxidative Stress. Induced by High Fat in BRL-3A Cells. Nanosci Nanotechnol Lett 2020; 12: 715–719.
  43. Zhang D, Lu Z, Sun B. Highly Sensitive Gold Nanoparticle Polymerase Chain Reaction in the Detection of Anaplastic Lymphoma Kinase-Positive Gastric Cancer from a Biopsy Specimen. Nanosci Nanotechnol Lett 2020; 12(4):498-505.
  44. Kazemi E, Zargooshi J Kaboudi M, Heidari P, Kahrizi D, Mahaki B, Mohammadian Y, Khazaei H, Ahmed K. A genome-wide association study to identify candidate genes for erectile dysfunction. Brief Bioinform 2020; bbaa338, https://doi.org/10.1093/bib/bbaa338.
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