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Vol. 66 No. 5: Issue 5

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Knockdown of Angiopoietin-like protein 4 suppresses the development of colorectal cancer

https://doi.org/10.14715/cmb/2020.66.5.21
Liqiang Wen
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of gastrointestinal surgery, Sun YAT-SEN Memorial Hospital, Sun YAT-SEN University, NO.107 Yan Jiang Road West, Guangzhou 510120, China
Yuchao Zhang
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of gastrointestinal surgery, Sun YAT-SEN Memorial Hospital, Sun YAT-SEN University, NO.107 Yan Jiang Road West, Guangzhou 510120, China
Bin Yang
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of gastrointestinal surgery, Sun YAT-SEN Memorial Hospital, Sun YAT-SEN University, NO.107 Yan Jiang Road West, Guangzhou 510120, China
Fanghai Han
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of gastrointestinal surgery, Sun YAT-SEN Memorial Hospital, Sun YAT-SEN University, NO.107 Yan Jiang Road West, Guangzhou 510120, China
Abdol Ghaffar Ebadi
Department of Agriculture, Jouybar branch, Islamic Azad University, Jouybar, Iran
Mohsen Toughani
Department of Fishery, Babol branch, Islamic Azad University, Babol, Iran

Corresponding Author(s) : Fanghai Han

Hanfanghai_sysu@163.com

Cellular and Molecular Biology, Vol. 66 No. 5: Issue 5

Abstract

Colorectal cancer, is the growth of cancer cells in the part of the colon. Angiopeptin is one of the growth factors in the human body that is particularly effective in the regulatory process. In this research, the regulatory role and its mechanism of Angiopoietin-like protein 4 (ANGPTL4) in colorectal cancer (CRC) metastasis, has been studied. Protein expression of ANGPLT4 was analyzed by immunohistochemistry in tumor samples and adjacent normal specimens of 40 patients with CRC cancer of various phases. A gene knockout test was conducted, two effective siRNA of ANGPTL4, named siRNA1 and siRNA2, were constructed and transfected into two CRC cell lines SW480 and HT-29 to block the expression of ANGPTL4. QRT-PCR and western blotting were used to validate the knockdown efficiency of the mRNA and proteins. Based on the results, the protein expression of ANGPTL4 was increased in human CRC tissues with the development of CRC. Knockdown of ANGPTL4 by siRNA in SW480 and HT-29 cells in vitro inhibited cell proliferation, promoted cell apoptosis, and suppressed the ability of cell migration and invasion. Besides, the sensitivity of CRC cells to Cisplatin was increased in the low ANGPTL4 expression group. ANGPTL4 might be a new potential therapeutic target for patients with CRC.

 

Keywords

Angiopoietin-like 4 Colorectal cancer Proliferation Metastasis Invasion Cisplatin
Wen, L., Zhang, Y., Yang, B., Han, F., Ebadi, A. G., & Toughani, M. (2020). Knockdown of Angiopoietin-like protein 4 suppresses the development of colorectal cancer. Cellular and Molecular Biology, 66(5), 117–124. https://doi.org/10.14715/cmb/2020.66.5.21
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