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Vol. 66 No. 2: Issue 2

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Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro

https://doi.org/10.14715/cmb/2020.66.2.27
Qingxia Tao
Department of Neurosurgery, Affiliated Bayi Brain Hospital, the Seventh Medical Center of PLA General Hospital, Beijing, 100700, People's Republic of China
Cuiying Wu
Department of Neurosurgery, Affiliated Bayi Brain Hospital, the Seventh Medical Center of PLA General Hospital, Beijing, 100700, People's Republic of China
Xinda Li
Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China
Wenjin Chen
Department of Neurosurgery, Affiliated Bayi Brain Hospital, the Seventh Medical Center of PLA General Hospital, Southern Medical University, Guangzhou, 100000, People's Republic of China
Kai Sun
Department of Neurosurgery, Affiliated Bayi Brain Hospital, the Seventh Medical Center of PLA General Hospital, Army Medical University, Chongqing, 400038, People's Republic of China
Peng Zhang
Department of Neurosurgery, Affiliated Bayi Brain Hospital, the Seventh Medical Center of PLA General Hospital, Beijing, 100700, People's Republic of China
Zhijun Yang
Department of Neurosurgery, Affiliated Bayi Brain Hospital, the Seventh Medical Center of PLA General Hospital, Beijing, 100700, People's Republic of China
Ning Liu
Department of Neurosurgery, Affiliated Bayi Brain Hospital, the Seventh Medical Center of PLA General Hospital, Beijing, 100700, People's Republic of China
Ruxiang Xu
Department of Neurosurgery, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan, China
Tao Xu
Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China
Chong Wang
Affliated Zhoupu Hospital, Shanghai University of Medicine and Health Sciences, Shanghai 310115, People's Republic of China

Corresponding Author(s) : Ruxiang Xu

jhwq26@163.com

Cellular and Molecular Biology, Vol. 66 No. 2: Issue 2

Abstract

The aim of this study was to investigate the effect of three-dimensional (3D) bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells (HUMSCs) on cell viability, proliferation and differentiation in vitro. Functional 3D bio-printed microspheres consisting of HUMSCs were constructed using electrostatic inkjet technique. The parameters used for the synthesis of 3D bio-printed tissue constructs were first optimized. The viability, proliferation and differentiation of 3D cultured HUMSCs were assessed. The results of scanning electron microscopy (SEM) showed that isolated HUMSCs exhibited fibroblast-like spindle adherent growth. The optimized printing parameters were 6 kV voltage, 10 mL/h flow, 15 cm receiving height, and alginate: water ratio of 1:1 mixed at 37 °C. Compared with 2D cultured HUMSCs, the 3D cultured HUMSCs have better viability, proliferation and differentiation ability. The results obtained in this study indicate that 3D bio-printed tissue constructs promote HUMSC viability, proliferation, and neural differentiation in vitro.

Keywords

Human umbilical-derived mesenchymal stem cell Three-dimensional bioprinted microspheres Viability Proliferation Bio-electrospraying.
Tao, Q., Wu, C., Li, X., Chen, W., Sun, K., Zhang, P., Yang, Z., Liu, N., Xu, R., Xu, T., & Wang, C. (2020). Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro. Cellular and Molecular Biology, 66(2), 165–171. https://doi.org/10.14715/cmb/2020.66.2.27
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