Effect of native growth promoting bacteria and commercial biofertilizers on growth and yield of wheat (Triticum aestivum) and barley (Hordeum vulgare) under salinity stress conditions

Tahereh Emami; Mohammad Mirzaeiheydari; Abbas Maleki; Masoud Bazgir

doi:10.14715/cmb/2019.65.6.5

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Vol. 65 No. 6: Issue 6

Issue Published : August 5, 2019

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Effect of native growth promoting bacteria and commercial biofertilizers on growth and yield of wheat (Triticum aestivum) and barley (Hordeum vulgare) under salinity stress conditions

https://doi.org/10.14715/cmb/2019.65.6.5

Tahereh Emami

Department of Agronomy and Plant Breeding, College of Agriculture, Ilam Branch, Islamic Azad University, Ilam, Iran

Mohammad Mirzaeiheydari

Department of Agronomy and Plant Breeding, College of Agriculture, Ilam Branch, Islamic Azad University, Ilam, Iran

Abbas Maleki

Department of Agronomy and Plant Breeding, College of Agriculture, Ilam Branch, Islamic Azad University, Ilam, Iran

Masoud Bazgir

Department of Soil and Water Engineering, Faculty of Agriculture, Ilam University, Ilam, Iran

Corresponding Author(s) : Mohammad Mirzaeiheydari

mohammad_mirzae@yahoo.com

Cellular and Molecular Biology, Vol. 65 No. 6: Issue 6
Article Published : August 5, 2019

Abstract

Salinity is one of the main obstacles to the production of crops in dry regions of the world. This study focuses on the effects of different strains of plant growth promoting rhizobacteria (PGPR) isolated from native soils on the physiological responses of wheat and barley plants under normal and salt stress conditions. Soil samples were collected from a field in Ilam province, in Iran and bacterial isolates were isolated and screened for salt tolerance, included siderophore and ACC-deaminase production and phosphate solubilizing. Thereafter a two-years greenhouse experiment was conducted as a completely randomized block design with four replications. The applied treatments included bacterial inoculation at five levels (B0: non-inoculation, B1: Siderophore producing + salt-tolerant bacteria, B2: phosphate solubilizing + salt-tolerant bacteria, B3: ACC-deaminase producing + salt-tolerant bacteria, B4: Barvar-2 biological fertilizer, B5: Biofarm-2 biological fertilizer) and salt stress at three levels (S1: 0 dS/m, S2: 4 dS/m, S3: 8 dS/m). Results showed that phosphate solubilizing+ salt-tolerant bacteria resulted in the highest barley grain yield at 4 dS/m salinity level and had no significant difference with ACC-deaminase producing + salt-tolerant bacteria and Barvar-2 biological fertilizer and Biofarm-2 biological fertilizer. The highest proline content in wheat and barley observed in Siderophore producing+ salt-tolerant bacteria at 8 dS/m by 17.48 and 23.42, respectively, followed by phosphate solubilizing+ salt-tolerant bacteria by 16.53 and 19.78. Therefore, the application of isolated growth promoting bacteria can be recommended as an effective biofertilizer in Ilam province.

Keywords

Physiological traits Promotion Microorganisms Tolerance.

Emami, T., Mirzaeiheydari, M., Maleki, A., & Bazgir, M. (2019). Effect of native growth promoting bacteria and commercial biofertilizers on growth and yield of wheat (Triticum aestivum) and barley (Hordeum vulgare) under salinity stress conditions. Cellular and Molecular Biology, 65(6), 22–27. https://doi.org/10.14715/cmb/2019.65.6.5

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References

References
Tiwari JK, Munshi AD, Kumar R, Pandey RN, Arora A, Bhat JS, Sureja AK. Effect of salt stress on cucumber: Na+-K+ ratio, osmolyte concentration, phenols and chlorophyll content. Acta Physiol. Plant 2010; 32: 103-114.
Saghafi K, Ahmadi J, Asgharzadeh A, Bakhtiari SH. The effect of microbial inoculants on physiological responses of two wheat cultivars under salt stress. International journal of Advanced Biological and Biomedical Research 2013; 1(4): 421-431.
Parida AK, Das AB. Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety 2005; 60(3): 324-349.
Dimkpa C, Weinand T, Ash F. Plant-rhizobacteria interactions alleviate abiotic stress conditions. Plant, Cell and Environment 2009; 32: 1682-1694.
Hayat R, Ali S, Amara U, Khalid R, Ahmed I. Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of Microbiology 2010; 60: 579-598.
Beneduzi A, Peres D, Vargas LK, Bodanese-Zanettini MH, Passaglia LMP. Evaluation of genetic diversity and plant growth promoting activities of nitrogen-fixing bacilli isolated from rice fields in South Brazil. Appl Soil Ecol 2008; 39:311-320.
Ambrosini A, Beneduzi A, Stefanski T, Pinheiro FG, Vargas LK, Passaglia LMP. Screening of plant growth promoting rhizobacteria isolated from sunflower (Helianthus annuus L.). Plant Soil 2012; 356:245-264.
Desouza R, Ambrosini A, Passaglia LMP. Plant growth-promoting bacteria as inoculants in agricultural soils. Genetic Molecular Biology 2015; 38(4): 401-409.
Antoun H, Prévost D. Ecology of plant growth promoting rhizobacteria. In: Siddiqui, ZA (ed), PGPR: Biocontrol and Bio fertilization. Springer, Dordrecht, The Netherlands 2006; pp.1-38.
Xiaofang W, Wei Zh, Li M, Wang X, Shan A, Mei X, Jousset A, Shen Q, Xu Y, Petri-Friman V. Parasites and competitors suppress bacterial pathogen synergistically due to evolutionary trade"offs. International Journal of Organic Evolution 2017; 71(3): 733-746.
Weyens N, Van der Lelie D, Taghavi S, Newman L, Vangronsveld J. Exploiting plant–microbe partnerships to improve biomass production and remediation. Trends Biotechnology 2009; 27(10):591–598.
Guzel RK, Lidiya BV, Tatiana NA, Ludmila YK, Nilya FG, Ludmila VS, Ilusa MG, Alexander IM, Sanislav YV. Effect of auxin producing and phosphate solubilizing bacteria on mobility of soil phosphorus, growth rate, and P acquisition by wheat plants. Acta Physiol Plant 2017; 39:253-261.
Amico ED, Cavalca L, Andreoni V. Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiology and Ecology 2005; 52: 153-162.
Alexander DB, Zuberer DA. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biology and Fertility of Soils 1991; 12: 39-45.
Schmidt DD, Kessler A, Kessler D, Schmidt S, Lim M. (Solanum nigrum) A model ecological expression system and its tools. Molecular Ecology 2004; 13: 981–995.
Gholizadeh A, Dehghania H, Dvorakb J. Determination of the most effective traits on wheat yield under saline stress. Agricultural Advances 2014; 3: 103–110.
Zinniel DK, Lambrecht P, Harris NB, Feng Z, Kuczmarski D, Higley P, Ishimaru CA, Arunakumari A, Barletta RG, Vidaver AK. Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Appl Env Microbiol 2002; 68: 2198–2208.
Kumar A, Maurya BR, Raghuwanshi R. Isolation and Characterization of PGPR and their effect on growth, yield and nutrient content in wheat (Triticum aestivum L.). Biocatal Agric Biotechnol 2014; 3:121–128.
Contesto C, Milesi S, Mantelin S, Zancarini A, Desbrosses G, Varoquaux F, Bellini C, Kowalczyk M, Touraine B. The auxin-signaling pathway is required for the lateral root response of Arabidopsis to the rhizobacterium Phyllobacterium brassicacearum. Planta 2010; 232:1455–1470.
Kumar A, Verma H, Singh VK, Singh PP, Singh SK, Ansari WA, Yadav A, Singh PK, Pandey KD. Role of Pseudomonas sp. in Sustainable Agriculture and Disease Management. In Agriculturally Important Microbes for Sustainable Agriculture. Springer, Singapore 2017; pp. 195–215.
Renato de Freitas J. Yield and N assimilation of winter wheat (Triticumaestivum L., var. Norstar) inoculated with rhizobacteria. Pedobiol 2000; 44: 97–104.
Zahir AZ, Ghani U, Naveed M, Nadeem SM, Asghar HN. Comparative effectiveness of (Pseudomonas) and (Serratia sp.) containing ACC-deaminase for improving growth and yield of wheat (Triticumaestivum L.) under salt-stressed conditions. Arch Microbiol 2009; 191:415–424.
Munns R. Physiological processes limiting plant growth in saline soil: some dogmas and hypotheses. Plant, Cell & Environment 1993; 16: 15–24.
Sultana W, Begum F, Saifuzzaman M, Nessa A, Salahuddin ABM. Salt tolerance of three barley cultivars of early growth stage. Bang. J. Sci. Tech. 1999; 1(1): 29-34.
Lehmann S, Funck D, Szabados L, Rentsch D. Proline metabolism and transport in plant development. Amino Acids 2010; 39:949–962.
Kudoyarova GR, Melentiev AI, Martynenko EV, Arkhipova TN, Shendel GV, Kuzmina LU, Dodd IC, Veselov SU. Cytokinin producing bacteria stimulate amino acid deposition by wheat roots. Plant Physiol Biochem 2014; 83:285–291.
Demir I, Mazi K. Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages. Brazilian Archives in Biology and Technology 2008; 51: 897-902.
Han HS, Lee KD. Phosphate and potassium solubilizing bacteria effect on mineral uptake, soil availability and growth of Eggplant. Res. J. Agric. & Biol. Sci 2005; 1(2): 176-180.
Hosseinzadeh F, Sate A, Ramezanpour MR. Effects of Mycorrhiza and Plant Growth Promoting Rhizobacteria on Growth, Nutrients Uptake and Physiological Characteristics in (Calendula Officinalis L.) Middle East. J. Sci. Res. 2011; 8(5): 947- 953.
Gholami S, Shahsavani A, Nezarat S. The Effect of Plant Growth Promoting Rhizobacteria (PGPR) on Germination, Seedling Growth and Yield of Maize (Zea mays L.) World Academy of Science, Eng. Technol 2009; 49: 19- 24.

References

Tiwari JK, Munshi AD, Kumar R, Pandey RN, Arora A, Bhat JS, Sureja AK. Effect of salt stress on cucumber: Na+-K+ ratio, osmolyte concentration, phenols and chlorophyll content. Acta Physiol. Plant 2010; 32: 103-114.

Saghafi K, Ahmadi J, Asgharzadeh A, Bakhtiari SH. The effect of microbial inoculants on physiological responses of two wheat cultivars under salt stress. International journal of Advanced Biological and Biomedical Research 2013; 1(4): 421-431.

Parida AK, Das AB. Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety 2005; 60(3): 324-349.

Dimkpa C, Weinand T, Ash F. Plant-rhizobacteria interactions alleviate abiotic stress conditions. Plant, Cell and Environment 2009; 32: 1682-1694.

Hayat R, Ali S, Amara U, Khalid R, Ahmed I. Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of Microbiology 2010; 60: 579-598.

Beneduzi A, Peres D, Vargas LK, Bodanese-Zanettini MH, Passaglia LMP. Evaluation of genetic diversity and plant growth promoting activities of nitrogen-fixing bacilli isolated from rice fields in South Brazil. Appl Soil Ecol 2008; 39:311-320.

Ambrosini A, Beneduzi A, Stefanski T, Pinheiro FG, Vargas LK, Passaglia LMP. Screening of plant growth promoting rhizobacteria isolated from sunflower (Helianthus annuus L.). Plant Soil 2012; 356:245-264.

Desouza R, Ambrosini A, Passaglia LMP. Plant growth-promoting bacteria as inoculants in agricultural soils. Genetic Molecular Biology 2015; 38(4): 401-409.

Antoun H, Prévost D. Ecology of plant growth promoting rhizobacteria. In: Siddiqui, ZA (ed), PGPR: Biocontrol and Bio fertilization. Springer, Dordrecht, The Netherlands 2006; pp.1-38.

Xiaofang W, Wei Zh, Li M, Wang X, Shan A, Mei X, Jousset A, Shen Q, Xu Y, Petri-Friman V. Parasites and competitors suppress bacterial pathogen synergistically due to evolutionary trade"offs. International Journal of Organic Evolution 2017; 71(3): 733-746.

Weyens N, Van der Lelie D, Taghavi S, Newman L, Vangronsveld J. Exploiting plant–microbe partnerships to improve biomass production and remediation. Trends Biotechnology 2009; 27(10):591–598.

Guzel RK, Lidiya BV, Tatiana NA, Ludmila YK, Nilya FG, Ludmila VS, Ilusa MG, Alexander IM, Sanislav YV. Effect of auxin producing and phosphate solubilizing bacteria on mobility of soil phosphorus, growth rate, and P acquisition by wheat plants. Acta Physiol Plant 2017; 39:253-261.

Amico ED, Cavalca L, Andreoni V. Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiology and Ecology 2005; 52: 153-162.

Alexander DB, Zuberer DA. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biology and Fertility of Soils 1991; 12: 39-45.

Schmidt DD, Kessler A, Kessler D, Schmidt S, Lim M. (Solanum nigrum) A model ecological expression system and its tools. Molecular Ecology 2004; 13: 981–995.

Gholizadeh A, Dehghania H, Dvorakb J. Determination of the most effective traits on wheat yield under saline stress. Agricultural Advances 2014; 3: 103–110.

Zinniel DK, Lambrecht P, Harris NB, Feng Z, Kuczmarski D, Higley P, Ishimaru CA, Arunakumari A, Barletta RG, Vidaver AK. Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Appl Env Microbiol 2002; 68: 2198–2208.

Kumar A, Maurya BR, Raghuwanshi R. Isolation and Characterization of PGPR and their effect on growth, yield and nutrient content in wheat (Triticum aestivum L.). Biocatal Agric Biotechnol 2014; 3:121–128.

Contesto C, Milesi S, Mantelin S, Zancarini A, Desbrosses G, Varoquaux F, Bellini C, Kowalczyk M, Touraine B. The auxin-signaling pathway is required for the lateral root response of Arabidopsis to the rhizobacterium Phyllobacterium brassicacearum. Planta 2010; 232:1455–1470.

Kumar A, Verma H, Singh VK, Singh PP, Singh SK, Ansari WA, Yadav A, Singh PK, Pandey KD. Role of Pseudomonas sp. in Sustainable Agriculture and Disease Management. In Agriculturally Important Microbes for Sustainable Agriculture. Springer, Singapore 2017; pp. 195–215.

Renato de Freitas J. Yield and N assimilation of winter wheat (Triticumaestivum L., var. Norstar) inoculated with rhizobacteria. Pedobiol 2000; 44: 97–104.

Zahir AZ, Ghani U, Naveed M, Nadeem SM, Asghar HN. Comparative effectiveness of (Pseudomonas) and (Serratia sp.) containing ACC-deaminase for improving growth and yield of wheat (Triticumaestivum L.) under salt-stressed conditions. Arch Microbiol 2009; 191:415–424.

Munns R. Physiological processes limiting plant growth in saline soil: some dogmas and hypotheses. Plant, Cell & Environment 1993; 16: 15–24.

Sultana W, Begum F, Saifuzzaman M, Nessa A, Salahuddin ABM. Salt tolerance of three barley cultivars of early growth stage. Bang. J. Sci. Tech. 1999; 1(1): 29-34.

Lehmann S, Funck D, Szabados L, Rentsch D. Proline metabolism and transport in plant development. Amino Acids 2010; 39:949–962.

Kudoyarova GR, Melentiev AI, Martynenko EV, Arkhipova TN, Shendel GV, Kuzmina LU, Dodd IC, Veselov SU. Cytokinin producing bacteria stimulate amino acid deposition by wheat roots. Plant Physiol Biochem 2014; 83:285–291.

Demir I, Mazi K. Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages. Brazilian Archives in Biology and Technology 2008; 51: 897-902.

Han HS, Lee KD. Phosphate and potassium solubilizing bacteria effect on mineral uptake, soil availability and growth of Eggplant. Res. J. Agric. & Biol. Sci 2005; 1(2): 176-180.

Hosseinzadeh F, Sate A, Ramezanpour MR. Effects of Mycorrhiza and Plant Growth Promoting Rhizobacteria on Growth, Nutrients Uptake and Physiological Characteristics in (Calendula Officinalis L.) Middle East. J. Sci. Res. 2011; 8(5): 947- 953.

Gholami S, Shahsavani A, Nezarat S. The Effect of Plant Growth Promoting Rhizobacteria (PGPR) on Germination, Seedling Growth and Yield of Maize (Zea mays L.) World Academy of Science, Eng. Technol 2009; 49: 19- 24.

Author biographies is not available.