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Vol. 65 No. 4: Issue 4

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Delayed development influences the outcome of different grades of D5 and D6 blastocysts during freeze–thaw cycle

https://doi.org/10.14715/cmb/2019.65.4.1
Huina Zhang
Reproductive Medicine center, Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
Samuel Kofi Arhin
Reproductive Medicine center, Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
Junzhao Zhao
Reproductive Medicine center, Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
Xiaohong Hou
Reproductive Medicine center, Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
Yilu Chen
Reproductive Medicine center, Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
Zhaoxia Huang
Reproductive Medicine center, Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China

Corresponding Author(s) : Zhaoxia Huang

aurora917@126.com

Cellular and Molecular Biology, Vol. 65 No. 4: Issue 4

Abstract

To analyze the effects of blastocysts on the 5th day (D5) and 6th day (D6) of frozen–thawed blastocyst transplantation on pregnancy outcome and provide evidence for further improvement of the strategy. This study included transfers from the Reproductive Medicine Center of the Second Affiliated Hospital of Wenzhou Medical University during freeze–thaw cycles from January 2016 to December 2017. They were divided into D5 group (1616 cases) and D6 group (619 cases) according to blastocyst formation and development. Each group was further divided into 5 groups according to the quality of the blastocyst and the number of transplants, making a total of 10 groups. Following the frozen transplantation cycle, the transplanting rate was significantly higher for D5 (41.73%) than for D6 (23.98%) (P < 0.05); the ongoing pregnancy rate (47,40%) was also significantly higher than that of D6 (28.43%) (P < 0.05).In the frozen–thawed blastocyst resuscitation transplantation, compared to D6 blastocysts, D5 blastocysts were more conducive to blastocyst implantation and could be used to achieve better clinical pregnancy outcome. In blastocyst selection, a single D5 excellent blastocyst transplant is preferred. Only at the 6th day of non-excellent D6, 2 blastocysts are recommended for transplantation.

Keywords

Frozen–thawed blastocyst Vitrification Blastocyst transplant Clinical outcome Pregnancy rate.
Zhang, H., Arhin, S. K., Zhao, J., Hou, X., Chen, Y., & Huang, Z. (2019). Delayed development influences the outcome of different grades of D5 and D6 blastocysts during freeze–thaw cycle. Cellular and Molecular Biology, 65(4), 1–5. https://doi.org/10.14715/cmb/2019.65.4.1
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