Issue

Vol. 64 No. 14: Issue 14

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Protective effect of date extract on rat nephrotoxicity induced by gentamicin, clinical, histological and antioxidant evidences

https://doi.org/10.14715/cmb/2018.64.14.18
Ozgur Yasar Celik
Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey
Kivanc Irak
Department of Biochemistry, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey

Corresponding Author(s) : Ozgur Yasar Celik

oyc@siirt.edu.tr

Cellular and Molecular Biology, Vol. 64 No. 14: Issue 14

Abstract

In this study, it was aimed to investigate the effect of the date extract (Phoenix dactylifera) on certain biochemical parameters and total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index in nephrotoxicity induced by gentamicin. The rats used in the study were randomly selected and divided into 4 groups, each consisting of 8 rats: control group, date extract (DE) group, gentamicin (GEN) group, gentamicin+date extract (GEN+DE) group. Blood samples and kidney tissues were taken 24 hours after eight days of trial. Urea, Creatinine, BUN, Na, Cl and K analyzes on the serum samples were carried out in auto analyzer. One of the kidney tissues was examined histopathologically. The supernatant, which was obtained by homogenizing the other kidney tissue was used in TAS and TOS analyzes. OSI was calculated using the formula. Urea, Creatinine, and BUN levels were higher in the GEN group, when compared to the other groups (p<0.001), while Na (p<0.05), Cl and K levels (p<0.001) were lower than those of the other groups. When the control group and the GEN group were compared, it was observed that the level of TAS decreased in the renal tissue and the level of TAS increased in the GEN+DE group. It was determined that TOS (p<0.01) and OSI (p<0.001) levels increased in the GEN group and renal TOS and OSI levels decreased in the GEN+DE group when compared to the GEN group. In conclusion, when the histopathological changes in kidney tissue with antioxidant and oxidant status in nephrotoxicity with gentamicin are examined, it can be said that date extract with gentamicin attenuates nephrotoxicity caused by gentamicin and date extract protects the kidney.

Keywords

Biochemical parameters Gentamycin Date extract Nephrotoxicity Rat.
Celik, O. Y., & Irak, K. (2018). Protective effect of date extract on rat nephrotoxicity induced by gentamicin, clinical, histological and antioxidant evidences. Cellular and Molecular Biology, 64(14), 108–113. https://doi.org/10.14715/cmb/2018.64.14.18
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Ali B. Gentamicin nephrotoxicity in humans and animals: some recent research. General Pharmacology: The Vascular System 1995; 26(7): 1477-1487.
  2. Paterson DL, Robson J, Wagener MM. Risk factors for toxicity in elderly patients given aminoglycosides once daily. J. Gen. Intern. Med. 1998; 13(11): 735-739.
  3. Traş B, Yazar E, Elmas M. Veteriner ć°laç Rehberi Tedavi El Kitabı. Atlas Yayınevi., Konya, 2018.
  4. Al-Qarawi A, Abdel-Rahman H, Mousa H, Ali B, El-Mougy S. Nephroprotective action of Phoenix dactylifera. in gentamicin-induced nephrotoxicity. Pharm. Biol. 2008; 46(4): 227-230.
  5. Smetana S, Khalef S, Nitsan Z, Hurwitz N, Miskin A, Bar-Khayim Y, Birk Y. Enhanced urinary trypsin inhibitory activity in gentamicin-induced nephrotoxicity in rats. Clin. Chim. Acta 1988; 176(3): 333-342.
  6. Oseni TIA, Oseni NT, Oseni HT, Eromon PE. Effects of date fruit extract on paracetamol induced nephrotoxicity in wistar rats. Afr. J. Biochem. Res 2017; 11(4): 18-21.
  7. El-Far AH, Shaheen HM, El-Daim MA, Al Jaouni SK, Mousa JSA. Date palm (Phoenix dactylifera): protection and remedy food. Journal of Nutraceuticals and Food Science 2016; 1(2).
  8. Al-Alawi RA, Al-Mashiqri JH, Al-Nadabi JS, Al-Shihi BI, Baqi Y. Date palm tree (Phoenix dactylifera L.): natural products and therapeutic options. Front Plant Sci 2017; 8(845.
  9. Al-Harrasi A, Rehman NU, Hussain J, Khan AL, Al-Rawahi A, Gilani SA, Al-Broumi M, Ali L. Nutritional assessment and antioxidant analysis of 22 date palm (Phoenix dactylifera) varieties growing in Sultanate of Oman. Asian Pac. J. Trop. Med. 2014; 7(S591-S598.
  10. Chao CT, Krueger RR. The date palm (Phoenix dactylifera L.): overview of biology, uses, and cultivation. HortScience 2007; 42(5): 1077-1082.
  11. Li Y, Kandasamy K, Chuah JKC, Lam YN, Toh WS, Oo ZY, Zink D. Identification of nephrotoxic compounds with embryonic stem-cell-derived human renal proximal tubular-like cells. Mol. Pharm. 2014; 11(7): 1982-1990.
  12. Zangiabadi N, Asadi-Shekaari M, Sheibani V, Jafari M, Shabani M, Asadi AR, Tajadini H, Jarahi M. Date fruit extract is a neuroprotective agent in diabetic peripheral neuropathy in streptozotocin-induced diabetic rats: a multimodal analysis. Oxid. Med. Cell. Longev. 2011; 2011(976948): 1-9.
  13. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 2004; 37(4): 277-285.
  14. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin. Biochem. 2005; 38(12): 1103-1111.
  15. Harma M, Harma M, Erel O. Increased oxidative stress in patients with hydatidiform mole. Swiss Med. Wkly. 2003; 133(41-42): 563-566.
  16. Kosecik M, Erel O, Sevinc E, Selek S. Increased oxidative stress in children exposed to passive smoking. Int. J. Cardiol. 2005; 100(1): 61-64.
  17. Yumru M, Savas HA, Kalenderoglu A, Bulut M, Celik H, Erel O. Oxidative imbalance in bipolar disorder subtypes: a comparative study. Prog. Neuropsychopharmacol. Biol. Psychiatry 2009; 33(6): 1070-1074.
  18. Kuwana H, Terada Y, Kobayashi T, Okado T, Penninger J, Irie-Sasaki J, Sasaki T, Sasaki S. The phosphoinositide-3 kinase γ–Akt pathway mediates renal tubular injury in cisplatin nephrotoxicity. Kidney Int. 2008; 73(4): 430-445.
  19. Kayaalp O Aminoglikozitler. in: Rasyonel tedavi yönünden tıbbi farmakoloji. (O. Kayaalp, Ed.^, Eds.), Hacettepe-Taş Kitapçılık Limited Åžti., Ankara, 2005.
  20. Mycek M, Howland R. Lippincott Illustrated Review of Pharmacology. Williams & Wilkins, Philadelphia, 2004.
  21. Doğan AL, Güç D. Sinyal iletimi mekanizmaları ve kanser. Acta Med. Cordoba 2004; 35(1): 34-42.
  22. Poyraz ć°. Cloning and enzymatic characterization of a mitogen-activated protein kinase kinase (MAPKK) enzyme family member in Origanum onites. Anadolu University, Biology, 2007, Eskişehir.
  23. Ali B, Al"Wabel N, Mahmoud O, Mousa H, Hashad M. Curcumin has a palliative action on gentamicin"induced nephrotoxicity in rats. Fundam. Clin. Pharmacol. 2005; 19(4): 473-477.
  24. Shifow A, Kumar K, Naidu M, Ratnakar K. Melatonin, a pineal hormone with antioxidant property, protects against gentamicin-induced nephrotoxicity in rats. Nephron 2000; 85(2): 167-174.
  25. Soliman KM, Abdul-Hamid M, Othman AI. Effect of carnosine on gentamicin-induced nephrotoxicity. Med. Sci. Monit. 2007; 13(3): BR73-BR83.
  26. Ural M, í–zgüner M, Åženal D, Sütçü R, Delibaş N. Siklosporin A'nın sıçanlarda oluşturduğu nefrotoksisiteye Vitamin C ile Vitamin E'nin ve verapamilin etkilerinin ışık mikroskobunda değerlendirilmesi. SDíœ Tıp Fakültesi Dergisi 2005; 12(4): 28-35.
  27. Hozayen W, Bastawy M, Elshafeey H. Effects of aqueous purslane (Portulaca oleracea) extract and fish oil on gentamicin nephrotoxicity in albino rats. Nat Sci 2011; 9(47-62.
  28. Yilmaz M, Mert H, Irak K, Erten R, Mert N. The Effect Of Fucoidan On The Gentamicin Induced Nephrotoxicity In Rats. Fresen Environ Bull 2018; 27(4): 2235-2241.
  29. Ataman N. The effect of fucoidan on the change of some biochemical parameteres in nephrotoxicity induced by gentamicin in rats. The University of Yüzüncü Yıl, Health Sciences Institute, Depertment of Biochemistry, MSci. Thesis, Van,, 2015.,
  30. Mehmetoglu ć°. Klinik Biyokimya El Kitabı. Nobel tıp Kitapevleri Ltd Åžti, Nobel tıp Kitapevleri Ltd Åžti, 2013.
  31. Cronin R, Thompson J. Role of potassium in the pathogenesis of acute renal failure. Miner. Electrolyte Metab. 1991; 17(2): 100-105.
  32. Silan C, Uzun í–, í‡omunoglu Níœ, Gokçen S, Bedirhan S, Cengiz M. Gentamicin-induced nephrotoxicity in rats ameliorated and healing effects of resveratrol. Biol. Pharm. Bull. 2007; 30(1): 79-83.
  33. Saafi EB, Louedi M, Elfeki A, Zakhama A, Najjar MF, Hammami M, Achour L. Protective effect of date palm fruit extract (Phoenix dactylifera L.) on dimethoate induced-oxidative stress in rat liver. Exp. Toxicol. Pathol. 2011; 63(5): 433-441.
  34. Vayalil PK. Antioxidant and antimutagenic properties of aqueous extract of date fruit (Phoenix dactylifera L. Arecaceae). J. Agric. Food Chem. 2002; 50(3): 610-617.
  35. Talih G. The analysis of the dexmedetomidine effect on colistin nephrotoxicity formed in rats. Erciyes University Medical Faculty, Anesthesiology and Reanimation, 2015, Kayseri.
  36. Koyuncu ć°. Ratlarda Sisplatin Kaynaklı Nefrotoksisite íœzerine Naringeninin Koruyucu Etkisinin ć°ncelenmesi. Harran íœniv Vet Fak Derg 2018; 7(1): 119-125.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 1160 Download : 505