Investigation of cochlear hair cells and the perception of ultrasound signals in guinea pigs

Fu-Sen Wang; Guohui Nie; Cheng-Cheng Huang; Qian Li; Tiegang Li; Yuee Zou; Shaoyan Zhang; Xinyu Ceng

doi:10.14715/cmb/2018.64.11.9

Issue

Vol. 64 No. 11: Issue 11

Issue Published : August 31, 2018

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Investigation of cochlear hair cells and the perception of ultrasound signals in guinea pigs

https://doi.org/10.14715/cmb/2018.64.11.9

Fu-Sen Wang

Department of Otorhinolaryngology, Southern Medical University Affiliated Shenzhen Baoan Hospital, Shenzhen, China

Guohui Nie

Department of Otorhinolaryngology, the Second People's Hospital of Shenzhen city, Shenzhen, China

Cheng-Cheng Huang

Department of Otorhinolaryngology, Wuhan General hospital of PLA, Wuhan, China

Qian Li

Chinese People's Liberation Army 301 Hospital ENT Research Institute, Beijing, China

Tiegang Li

Molecular Imaging Centre, Institute of Materia Medical, Chinese Academy of Medical Sciences, Beijing, China

Yuee Zou

Department of electrocardiogram, Southern Medical University Affiliated Shenzhen Baoan Hospital, Shenzhen, China

Shaoyan Zhang

Department of Otorhinolaryngology, Southern Medical University Affiliated Shenzhen Baoan Hospital, Shenzhen, China

Xinyu Ceng

Department of Otorhinolaryngology, Southern Medical University Affiliated Shenzhen Baoan Hospital, Shenzhen, China

Corresponding Author(s) : Fu-Sen Wang

gr1237@163.com

Cellular and Molecular Biology, Vol. 64 No. 11: Issue 11
Article Published : August 30, 2018

Abstract

We established a specific ultrasound frequency-dependent model of cochlear injury using bone conduction ultrasounds in the inner ear of guinea pigs at 50 kHz and 83 kHz, to explore the effects of bone conduction ultrasound in the cochlea. To establish a unilateral cochlear damage model, the unilateral cochlea was destroyed. The control group consisted of 50 kHz and 83 kHz bone conduction ultrasounds in unaltered guinea pigs. In each group, cerebral blood oxygenation level dependent (BOLD) effects were determined by functional magnetic resonance imaging (fMRI). The cochlear outer hair cell motor protein, Prestin, and the microfilament protein, F-Actin, were detected. We found that bone conduction ultrasound irradiation at 50 kHz and 83 kHz on the guinea pig inner ear for six hours leads to hair cell damage. Furthermore, low frequency bone conduction ultrasound induces major damage to outer hair cells, while high frequency ultrasound damages both internal and external hair cells. fMRI analysis of cerebral BLOD effects revealed an affected cerebral cortex region of interest (ROI) of 4 and 2, respectively, for the normal control group at 50 kHz or 83 kHz, and 2 for the 83 kHz bone conduction ultrasound cochlear injury group, while 50 kHz bone conduction ultrasound failed to induce the cortical ROI within injury model. Results reveal that the spatial location of guinea pig cochlear hair cells determines coding function for lower ultrasound frequencies, and high frequency bone conduction ultrasound may affect the cochlear spiral ganglion or cranial nerve nucleus in bone conduction ultrasound periphery perception.

Keywords

Cochlear hair cells Ultrasound signals Guinea pigs Cochlear damage model.

Wang, F.-S., Nie, G., Huang, C.-C., Li, Q., Li, T., Zou, Y., Zhang, S., & Ceng, X. (2018). Investigation of cochlear hair cells and the perception of ultrasound signals in guinea pigs. Cellular and Molecular Biology, 64(11), 44–49. https://doi.org/10.14715/cmb/2018.64.11.9

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References

Tsukano, H., Horie, M., Bo, T., Uchimura, A., Hishida, R., Kudoh, M., et al. Delineation of a frequency-organized region isolated from the mouse primary auditory cortex. J Neurophysiol 2015; 113: 2900–2920.
Du Y, Ping J, Li N, Wu X, Li L, Galbraith G. Ultrasonic evoked responses in rat cochlear nucleus. Brain Res 2007; 1172: 40-47.
Portfors CV, Perkel DJ. The role of ultrasonic vocalisations in mouse communication. Curr Opin Neurobiol 2014; 28: 115-120.
Ferhat AT, Torquet N, Le Sourd AM, de Chaumont F, Olivo-Marin JC, Faure P, et al. Recording mouse ultrasonic vocalisations to evaluate social communication. J Vis Exp 2016.
Schwarting RK, Wohr M. On the relationships between ultrasonic calling and anxiety-related behaviour in rats. Braz J Med Biol Res 2012; 45: 337-348.
Garcia-Lazaro JA, Shepard KN, Miranda JA, Liu RC, Lesica NA. An overrepresentation of high frequencies in the mouse inferior colliculus supports the processing of ultrasonic vocalisations. PLoS One 2015; 10: 133251.
Lau C, Zhang JW, Cheng JS, Zhou IY, Cheung MM, Wu EX. Non-invasive fMRI investigation of inter-aural level difference processing in the rat auditory subcortex. PLoS One 2013; 8: 70706.
Ohyama K, Kusakari J, Kawamoto K. Ultrasonic electrocochleography in guinea pig. Hear Res 1985; 17: 143-151.
Lenhardt ML, Skellett R, Wang P, Clarke AM. Human ultrasonic speech perception. Sci 1991; 253: 82-85.
Nishimura T, Okayasu T, Saito O, Shimokura R, Yamashita A, Yamanaka T, et al. An examination of the effects of broadband air-conduction masker on the speech intelligibility of speech-modulated bone-conduction ultrasound. Hear Res 2014; 317: 41-49.
Matsumoto J, Nishimaru H, Takamura Y, Urakawa S, Ono T, Nishijo H. Amygdalar auditory neurons contribute to self-other distinction during ultrasonic social vocalisation in rats. Front Neurosci 2016; 10: 399.
Nishimura T, Okayasu T, Uratani Y, Fukuda F, Saito O, Hosoi H. Peripheral perception mechanism of ultrasonic hearing. Hear Res 2011; 277: 176-183.
Okayasu T, Nishimura T, Yamashita A, Saito O, Fukuda F, Yanai S, et al. Human ultrasonic hearing is induced by a direct ultrasonic stimulation of the cochlea. Neurosci Lett 2013; 539: 71-76.
Torbatian Z, Garland P, Adamson R, Savage J, Bance M, Brown J. Listening to the cochlea with high-frequency ultrasound. Ultrasound Med Biol 2012; 38: 2208-2217.
Stiebler I. A distinct ultrasound-processing area in the auditory cortex of the mouse. Natwiss 1987; 74: 96-97.
Wang F, Wang J, Gong S. A study of the histologic and enzyme histochemical changes in the cochlea of guinea pigs after non-focused ultrasound irradiation. Cell Biochem Biophys 2013; 66: 409-415.

References

Tsukano, H., Horie, M., Bo, T., Uchimura, A., Hishida, R., Kudoh, M., et al. Delineation of a frequency-organized region isolated from the mouse primary auditory cortex. J Neurophysiol 2015; 113: 2900–2920.

Du Y, Ping J, Li N, Wu X, Li L, Galbraith G. Ultrasonic evoked responses in rat cochlear nucleus. Brain Res 2007; 1172: 40-47.

Portfors CV, Perkel DJ. The role of ultrasonic vocalisations in mouse communication. Curr Opin Neurobiol 2014; 28: 115-120.

Ferhat AT, Torquet N, Le Sourd AM, de Chaumont F, Olivo-Marin JC, Faure P, et al. Recording mouse ultrasonic vocalisations to evaluate social communication. J Vis Exp 2016.

Schwarting RK, Wohr M. On the relationships between ultrasonic calling and anxiety-related behaviour in rats. Braz J Med Biol Res 2012; 45: 337-348.

Garcia-Lazaro JA, Shepard KN, Miranda JA, Liu RC, Lesica NA. An overrepresentation of high frequencies in the mouse inferior colliculus supports the processing of ultrasonic vocalisations. PLoS One 2015; 10: 133251.

Lau C, Zhang JW, Cheng JS, Zhou IY, Cheung MM, Wu EX. Non-invasive fMRI investigation of inter-aural level difference processing in the rat auditory subcortex. PLoS One 2013; 8: 70706.

Ohyama K, Kusakari J, Kawamoto K. Ultrasonic electrocochleography in guinea pig. Hear Res 1985; 17: 143-151.

Lenhardt ML, Skellett R, Wang P, Clarke AM. Human ultrasonic speech perception. Sci 1991; 253: 82-85.

Nishimura T, Okayasu T, Saito O, Shimokura R, Yamashita A, Yamanaka T, et al. An examination of the effects of broadband air-conduction masker on the speech intelligibility of speech-modulated bone-conduction ultrasound. Hear Res 2014; 317: 41-49.

Matsumoto J, Nishimaru H, Takamura Y, Urakawa S, Ono T, Nishijo H. Amygdalar auditory neurons contribute to self-other distinction during ultrasonic social vocalisation in rats. Front Neurosci 2016; 10: 399.

Nishimura T, Okayasu T, Uratani Y, Fukuda F, Saito O, Hosoi H. Peripheral perception mechanism of ultrasonic hearing. Hear Res 2011; 277: 176-183.

Okayasu T, Nishimura T, Yamashita A, Saito O, Fukuda F, Yanai S, et al. Human ultrasonic hearing is induced by a direct ultrasonic stimulation of the cochlea. Neurosci Lett 2013; 539: 71-76.

Torbatian Z, Garland P, Adamson R, Savage J, Bance M, Brown J. Listening to the cochlea with high-frequency ultrasound. Ultrasound Med Biol 2012; 38: 2208-2217.

Stiebler I. A distinct ultrasound-processing area in the auditory cortex of the mouse. Natwiss 1987; 74: 96-97.

Wang F, Wang J, Gong S. A study of the histologic and enzyme histochemical changes in the cochlea of guinea pigs after non-focused ultrasound irradiation. Cell Biochem Biophys 2013; 66: 409-415.

Author biographies is not available.