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Vol. 64 No. 4: Issue 4

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Effect of Tunceli garlic on some immunological parameters in Cyprinus carpio exposed to chlorpyrifos

https://doi.org/10.14715/cmb/2018.64.4.18
Ayşegül Pala
Faculty of Fisheries, Munzur University, Tunceli, Turkey
Engin Åžeker
Faculty of Fisheries, Munzur University, Tunceli, Turkey
M. Enis Yonar
Faculty of Fisheries, Firat University, Elazığ, Turkey

Corresponding Author(s) : M. Enis Yonar

meyonar@gmail.com

Cellular and Molecular Biology, Vol. 64 No. 4: Issue 4

Abstract

In this study, the effect of Tunceli garlic (Allium tuncelianium) on hemoglobin (Hb) level, oxidative radical production of neutrophils (Nitoblue tetrazolium assay-NBT activity) and total immunoglobulin (TI) content in carp (Cyprinus carpio) exposed to chlorpyrifos (CPF)  was investigated. The 96 hour LC50 value of CPF on C. carpio was calculated to as 0.230 mg/L. The fishes were exposed to sublethal concentration of chlorpyrifos (1/8 of LC50 value: 0.029 mg/L), and Tunceli garlic (20 and 40 g/kg diet) was simultaneously administered for 21 days. Blood samples were taken from the fishes at 7, 14 and 21 days and analysed to determine the Hb levels, the NBT activity and the TI content. There was a significant decrease in the Hb level, the NBT activity and the TI content of CPF-treated fish. However, Tunceli garlic reversed the Hb level, the NBT activity and the TI content. In conclusion, this study demonstrated that CPF had a negative effect on the immunological values of the fish. The simultaneous administration of Tunceli garlic was neutralised CPF-induced toxicity.

Keywords

Chlorpyrifos Cyprinus carpio Hematology Immunology.
Pala, A., Åžeker, E., & Yonar, M. E. (2018). Effect of Tunceli garlic on some immunological parameters in Cyprinus carpio exposed to chlorpyrifos. Cellular and Molecular Biology, 64(4), 108–112. https://doi.org/10.14715/cmb/2018.64.4.18
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References
  1. Yonar ME, Mişe Yonar S, í‡oban MZ, Eroğlu M. Antioxidant effect of propolis against exposure to chromium in Cyprinus carpio. Environ Toxicol 2014; 29(2):155-64.
  2. Monteiro DA, Alves de Almeida J, Rantin FT, Kalinin AL. Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comp Biochem Phys 200; 143(C): 141-9.
  3. Sakin, F, Ispir íœ, Mişe Yonar S, Yonar ME, Taysi R. Effect of short-term cypermethrin exposure on oxidant-antioxidant balance in the whole body of rainbow trout fry (Oncorhynchus mykiss). Fresen Environ Bull 2011; 20(10a):2806–9.
  4. Ural MÅž, Yonar ME, Mişe Yonar S. Protective effect of ellagic acid on oxidative stress and antioxidant status in Cyprinus carpio during malathion exposure. Cell Mol Biol 2015; 61(5): 58–63.
  5. Mişe Yonar S, Sakin F, Yonar ME, Ispir íœ, Kirici M. Oxidative stress biomarkers of exposure to deltamethrin in rainbow trout fry (Oncorhynchus mykiss). Fresen Environ Bull 2011; 20(8): 1931–5.
  6. Mişe Yonar S, Yonar ME, Ural MS. Antioxidant effect of curcumin against exposure to malathion in Cyprinus carpio. Cell Mol Biol 2017; 63(3): 68–72.
  7. Yonar ME. Chlorpyrifos-induced biochemical changes in Cyprinus carpio: Ameliorative effect of curcumin. Ecotoxicol Environ Saf 2018; 151: 49–54.
  8. Amitai G, Moorad D, Adani A, Doctor BP. Inhibition of acetylcholinesterase and butyrylcholinesterase by chlorpyrifos-oxon. Biochem Pharmacol, 1998; 56: 293–9.
  9. Narra MR, Rajender K, Reddy RR, Rao J V, Begum G. The role of vitamin C as antioxidant in protection of biochemical and haematological stress induced by chlorpyrifos in freshwater fish Clarias batrachus. Chemosphere 2015; 132: 172-8.
  10. Tilak KS, Veeraiah K, Ramanakumari GV. Toxicity and effect of chloropyriphos to the freshwater fish Labeo rohita (Hamilton). Neur Res 2001; 20: 438–45.
  11. Giron-Perez MI, Barcelos-Garcia R, Vidal-Chavez ZG, Romero-Banuelos CA, Robledo-Marenco ML. Effect of chlorpyrifos on the hematology and phagocytic activity of Nile Tilapia Cells (Oreochromis niloticus). Toxicol Mech Method 2006; 16(9): 495-9.
  12. El-Bouhy ZM, El-Nobi G, Reda RM, Ibrahim RE. Effect of ınsecticide "Chlorpyrifos" on immune response of Oreochromis niloticus. Zagazig Vet J 2016; 44(3): 196-204.
  13. Ndong D, Fall J. The effect of garlic (Allium sativum) on growth and immune responses of hybrid tilapia (Oreochromis niloticus x Oreochromis aureus). J Clini Immunol Immunopathol Res 2011; 3(1):1-9.
  14. Ekim T, Koyuncu M, Duman H, Aytaç Z, Adıgüzel N. Türkiye Bitkileri Kırmızı Kitabı/ /Red Data Book of Turkish Plants 2000 Ankara.
  15. Taşkın H, Baktemur G, Kurul M, Buyukalaca S. Use of tissue culture techniques for producing virus-free plant in garlic and their identification through real-time PCR. The Scientific World Journal 2013; 5p.
  16. Talpur AD, Ikhwanuddin M. Dietary effects of garlic (Allium sativum) on haemato-immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian sea bass, Lates calcarifer (Bloch). Aquaculture 2012; 364-365: 6–12
  17. Guo JJ, Kuo CM, Hong JW, Chou RL, Lee YH, Chen TI. The effects of garlic-supplemented diets on antibacterial activities against Photobacterium damselae subsp. piscicida and Streptococcus iniae and on growth in Cobia, Rachycentron canadum. Aquaculture 2015; 435: 111–5.
  18. El-Barbary MI. Detoxification and antioxidant effects of garlic and curcumin in Oreochromis niloticus injected with aflatoxin B1 with reference to gene expression of glutathione peroxidase (GPx) by RT-PCR. Fish Physiol Biochem 2016; 42: 617–629.
  19. Ghehdarijani MS, Hajimoradloo A, Ghorbani R, Roohi Z. The effects of garlic-supplemented diets on skin mucosal immune responses, stress resistance and growth performance of the Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol 2016; 49: 79-83.
  20. Seker E, Akkan S. Effect of Tunceli garlic (Allium tuncelianum) on the haematological and immunological parameters of scaly carp (Cyprinus carpio). International Journal of Recent Trends in Science and Technology 2016 21(2): 122-8.
  21. Drabkin DL. Spectrophotometrıc studies: XIV. The crystallographic and optical properties of the hemoglobin of man in comparison wıth those of other species. J Biol Chem 1946; 164: 703-23.
  22. Siwicki AK, Anderson DP, Dixon OW. Dietary intake of immunostimulants by rainbow trout affects nonspecific immunity and protection against furunculosis. Vet Immunolo Immunop 1994; 41: 125–39.
  23. Siwicki AK, Anderson DP. Immunostimulation in fish: measuring the effects of stimulants by serological and immunological methods. US Fish Wild Service-IFI 1993; 1:1-17.
  24. Saglam N, Ispir U, Yonar M.E. The Effect of Therapeutic Bath of Malachite Green on Some Haematological Parameters of Rainbow trout Oncorhynchus mykiss Walbaum 1792. Fresen Environ Bull 2003; 12(10): 1207-10.
  25. Fazlolahzadeh F, Keramati K, Nazifi S, Shirian S, Seifi S. Effect of Garlic (Allium sativum) on hematological parameters and plasma activities of ALT and AST of rainbow trout in temperature stress. Australian Journal of Basic and Applied Sciences 2011; 5(9): 84-90.
  26. Mişe Yonar S, Yonar ME, Yöntürk Y, Pala A. Effect of ellagic acid on some haematological, immunological and antioxidant parameters of rainbow trout (Oncorhynchus mykiss). J Anim Physiol An. N 2014; 98: 936–41.
  27. Yonar ME, Mişe Yonar S, Pala A, Silici S, Saglam N. Trichlorfon-induced haematological and biochemical changes in Cyprinus carpio: ameliorative effect of propolis. Dis Aquat Organ 2015; 114: 209–16.
  28. Celik EÅž, Akbulut M, Odabaşi S, Odabaşi DA. Farkli tür baliklarda hematolojik indekslerin referans değerleri/ Reference values of haematological indices for different fish species. Anadolu University Journal of Science and Technology 2006; 2: 277-93.
  29. Yonar ME, Mişe Yonar S, Ural MÅž, Silici S, Düşükcan M. Protective role of propolis in chlorpyrifos-induced changes in the haematological parameters and the oxidative/antioxidative status of Cyprinus carpio carpio. Food Chem Toxicol 2012; 50: 2703-8.
  30. Ural MS. Chlorpyrifos-induced changes in oxidant/antioxidant status and haematological parameters of Cyprinus carpio carpio: Ameliorative effect of lycopene. Chemosphere 2013; 90: 2059-64.
  31. Aly SM, Attı NMA, Mohamed MF. Effect of garlic on the survival, growth, resistance and quality of Oreochromıs nıloticus. 18th International Symposium on Tilapia in Aquaculture 2008.
  32. Sakin F, Yonar ME, Mişe Yonar S, Saglam N. Changes in selected immunological parameters and oxidative stress responses in different organs of Oncorhynchus mykiss exposed to ivermectin. Rev Chim-Bucharest 2012; 63(10): 989-95.
  33. Mişe Yonar S. Toxic effects of malathion in carp, Cyprinus carpio carpio: Protective role of lycopene. Ecotoxicol Environ Saf 2013; 97: 223–9.
  34. Kaya H, í‡elik ÅžE, Yılmaz S, Tulgar A, Akbulut M, Demir N. Hematological, serum biochemical, and immunological responses in common carp (Cyprinus carpio) exposed to phosalone. Comp Clin Pathol 2015; 24: 497–507.
  35. Yonar ME, Mise Yonar S. Changes in selected immunological parameters and antioxidant status ofrainbow to malachite green (Oncorhynchus mykiss, Walbaum, 1792). Pestic Biochemy Phys 2010; 97:19–23.
  36. Li X, Liu L, Zhang Y, Fang Q, Li Y, Li Y. Toxic effects of chlorpyrifos on lysozyme activities, the contents of complement C3 and IgM, and IgM and complement C3 expressions in common carp (Cyprinus carpio L.). Chemosphere 2013; 93: 428–33.
  37. Ayaz E, Alpsoy HC. Garlic (Allium sativum) and Traditional Medicine. Turkish Society for Parasitology 2007; 31(2): 145-9.
  38. Banaee M, Haghi BM, Ibrahim ATA. Sub-lethal toxicity of chlorpyrifos on Common carp, Cyprinus carpio (Linnaeus, 1758) biochemical response. International Journal of Aquatic Biology 2013; 1(6): 281-8.
  39. Ota N, Takano F, Muroga S, Kawabata T, Ishigaki Y, Yahagi N, Ohta T. Garlic extract and its selected organosulphur constituents promote ileal immune responses ex vivo. Journal of Functional Foods 2012; 4(1): 243–52.
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