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Vol. 64 No. 14: Issue 14

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Investigation of association between CD40 current gene variants (rs4810485, rs1883832 and rs3765459) and serum CD154 protein levels in Iranian migraineurs

https://doi.org/10.14715/cmb/2018.64.14.12
Norullah Ramroodi
Department of Neurology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Hadi Saboori
Department of Statistics, Faculty of Sciences, University of Zabol, Zabol, Iran
Nima Sanadgol
Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran
http://orcid.org/0000-0002-4509-5336

Corresponding Author(s) : Nima Sanadgol

sanadgol.n@gmail.com

Cellular and Molecular Biology, Vol. 64 No. 14: Issue 14

Abstract

Migraine is a chronic neurological disease described by recurrent moderate to severe headaches often in association with neuro-inflammation. As cytokines are affect the immune response and migraine exacerbation, the current study aimed to investigate the possible associations between CD40 polymorphisms and level of soluble CD154 protein with migraine. In a prospective case-control study, we studied blood samples of 190 patients with migraine (migraineurs) and 200 healthy controls (HCs) from southeast Iran. Genotyping for the CD40 (rs4810485-intron, rs1883832-5´-UTR, and rs3765459-intron) gene variants were executed using PCR-RFLP and soluble CD154 protein levels were measured via ELISA method. Among CD40 gene variants, rs1883832 (TC genotype) was significantly associated with migraine (P = 0.007, OR = 2.326, 95% CI = 1.258-4.303).  No significant associations observed between the rs4810485 and rs3765459 SNPs with migraine. The most frequent genotypes for CD40 were GG in rs4810485 (51.5%) and rs3765459 (62.1%) as well as TC in rs1883832 (53.7%). There was no statistically relationship between these gene variants and different subclasses of migraine. Concentration of soluble CD40L among patients with rs1883832 (TC genotype) were significantly (P = 0.027, OR = 0.417, CI = 0.192-0.906) higher in compared to healthy controls. Our findings showed that in CD40 rs1883832, TC genotype may have a role in migraine susceptibility. Therefore, it suggested that in addition to other factors, CD40 rs1883832 (TC genotype) genetic variation may also play a critical role in the etiology of migraine.

Keywords

CD40 CD154 Inflammation Migraine Polymorphisms.
Ramroodi, N., Saboori, H., & Sanadgol, N. (2018). Investigation of association between CD40 current gene variants (rs4810485, rs1883832 and rs3765459) and serum CD154 protein levels in Iranian migraineurs. Cellular and Molecular Biology, 64(14), 72–78. https://doi.org/10.14715/cmb/2018.64.14.12
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Author Biographies

Norullah Ramroodi, Department of Neurology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

Assistant Professor

Nima Sanadgol, Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran
Assistant Professor
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