Issue

Vol. 63 No. 11: Issue 11

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Biochemical evaluation of hydroxyurea derivative schiff bases in liver of rats

https://doi.org/10.14715/cmb/2017.63.11.2
Akif Evren Parlak
Programme of Environmental Protection and Control, Keban Vocational School of Higher Education, Firat University, Elazig, Turkey
Yusuf Karagozoglu
Department of Chemistry, Faculty of Science and Arts, Bingol University, Bingol, Turkey
Naci O. Alayunt
Banaz Vocational School, Usak University, Usak, Turkey
Semra Turkoglu
Department of Nutrition and Dietetic, Health Sciences Faculty, Firat University, Elazig, Turkey
Isil Yildirim
Department of Chemistry,  Faculty of Science, Firat University, Elazig, Turkey
Mustafa Karatepe
Department of Chemistry,  Faculty of Science, Firat University, Elazig, Turkey
Metin Koparir
Department of Chemistry,  Faculty of Science, Firat University, Elazig, Turkey

Corresponding Author(s) : Semra Turkoglu

smrturkoglu@hotmail.com

Cellular and Molecular Biology, Vol. 63 No. 11: Issue 11

Abstract

In this study, it was aimed to examine the antioxidant and antihepatotoxic effects of hydroxyurea derivative Schiff bases on serum biochemical parameters (AST, ALT, LDH, urea, creatinine and total bilirubin) and antioxidant parameters (SOD, CAT, GPx, MDA). In this study, a total of 49 adult male Wistar rats was examined and they were divided into 7 equal groups. DMSO, which is diluted only with corn oil, was administered to control group. 25 mg / kg ligand, 25 mg / kg Schiff base - manganese, 25 mg / kg Schiff base-copper, 25 mg / kg Schiff base - zinc, 25 mg / kg Schiff base - nickel, 25 mg / kg Schiff base - cobalt complexes were administered to rats of experimental group subcutaneously for 15 days with three-day intervals throughout the test process. All specimens were killed by decapitation and their livers were extracted. According to the results obtained, ALT level was observed to be higher (P<0.05) in the Cu-L group compared to other groups. LDH level was observed to be higher (P<0.05) in the Cu-L and Co-L groups compared to other groups. SOD level was observed to be higher (P<0.05) in the Cu-L, Mn-L and Zn-L groups compared to other groups. MDA level was observed to be higher (P<0.05) in the Ni-L, Cu-L, Zn-L groups compared to other groups. In conclusion, it can be suggested that the determination of the pharmacological characteristics of them can be beneficial in numerous fields of application thanks to the antioxidant and hepatotoxic activities demonstrated by hydroxyurea derivative Schiff bases.

Keywords

Hydroxyurea Schiff bases Serum Biochemical parameters Antioxidant enzymes Liver.
Parlak, A. E., Karagozoglu, Y., Alayunt, N. O., Turkoglu, S., Yildirim, I., Karatepe, M., & Koparir, M. (2017). Biochemical evaluation of hydroxyurea derivative schiff bases in liver of rats. Cellular and Molecular Biology, 63(11), 5–10. https://doi.org/10.14715/cmb/2017.63.11.2
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Yang ZY, Yang RD, Li FS and Yu KB. Crystal Structure and antitumour Activity of some Rare Earth Metal Complexes with Schiff base. Polyhedron 2000; 19:2599-2064.
  2. Das A, Trousdale MD, Ren S and Lien EJ. Inhibition of Herbes Simplex Virus type 1 and Adeno Virus Type 5 by Heterocyclic SchifF base of Aminohydorxy Guanidine. Tosylate Antiviral Res.1999; 44:201-208.
  3. Sur B, Chatterjee SP, Sur P, Maity T and Roychoudhury S. Studies on the Antiplasticty of Schiff bases Containing 5- Nitrofuran and primidine. Oncology 1990; 47:433-438.
  4. Pires dos Santos ML, Alairo AF, Mangrich AS and Ferreira AMC. Antioxidant and Prooxidant Properties os some Di Schiff Base Copper(II) Complexes Journalof Inorganic. Biochem 1998; 71:71-78.
  5. Nishinaga A, Yamada T, Fujisawa H, Ishizaki K, Ihara H and Matsuura TJ. Catalysis by cobalt Schiff complexes in the oxygenation of alkenes on the mechanism of ketonization. Mol. Catal. 1988; 48:249-264.
  6. Zhao YD, Pang DW, Zong Z, Cheng JK, Luo ZF, Feng CJ, Shen HY and Zhung XC. Electochemical studies of antitumor drugs, fundamental electrochemical characteristics of an iron(III) Schiff base complex and its interaction with DNA. Hua Xue Xue Bao 1988; 56:178-183.
  7. Hadjipavlu-Litina D J and Geronikaki A A. Thiazolyl and benzothiazolyl Schiff base as novel possible lipoxygenase inhibitors and anti-inflammatory agents. Drug Des. Discov. 1998; 15: 199-206.
  8. De B and Ramasarma GVS. Determination of pK and correlation with the analgesic activity of 5-oximidazolyl amino pyrazole 4- carboxaldehdes and their Schiff bases. Indian Drugs 1999; 36:583-587.
  9. Luo X, Zhao J, Ling Y and Liu Z. Antioxidative effect of Schiff bases with o-hydroxybenzylidene group on free radical induced hemolysis of human red blood cell Chem. Res. Chinese Univ. 2002; 18:287-289.
  10. Ujiie T. Ann. Rept. Cancer Inst. Kanazawa Japn. 1967; 1:109.
  11. Williams DR. Metals, ligands, and cancer. Chem. Rev. 1972; 72:203-213.
  12. Albert A. Aust. J. Sci. 1967; 30:1.
  13. Metzler DE, Ikawa M and SneII EE. A general mechanism for vitamin B6-catalyzed reactions. J. Amer. Chem. Soc, 1954; 76:648.
  14. Dash B, Patra M and Praharaj S. Synthesis and biological activity of some schiff bases derived from thiazoles and benzothiazoles. Ind. J. Chem. 1980; 19B:894-897.
  15. Dash B, Patra M and Mahapatra PK. Ind. J. Chem. 1983; 60:772-774.
  16. Dash B and Mahapatra. SK. J. Inorg. Nucl. Chem. 1975; 37:271.
  17. Karatepe M and Karatas F. Antioxidant, Pro-Oxidant Effect of the Thiosemicarbazone Derivative Schiff Base (4-(1– Phenylmethylcyclobutane –3–yl)–2-((2Hydroxybenzylidenehydrazino)Thiazole ) and and its Metal Complexes on Rats. Cell Biochem. Func. 2006; 24:547-554.
  18. Varvaresou A, Tsantili-Kakoulidou A and Siatra-Papastaikoudi T, et al. Synthesis and biological evaluation of indole containing derivatives of thiosemicarbazide and their cyclic 1,2,4-triazole and 1,3,4-thiadiazole analogs. Arzneimittelforschung 2000; 50:48-54.
  19. Cesur N, Birteksöz S and í–tük G. Synthesis and Biological Evaluation of some new thiosemicarbazide, 4-thiazolidinone, 1,3,4-oxadiazole and l,2,4-triazole-3-thione derivatives bearing imidazo[l,2-a]pyridine moiety. Acta Pharm. Turcica 2002; 44:23-41.
  20. Turan N and Sekerci M. Synthesis and Characterization of Co (II), Ni (II), Cd (II) and Cu (II) Complexes of Bis-Schiff Bases obtained from 1,8-Diaminonaphthalene. J. Chem Soc. Pakistan 2009; 31:564-568.
  21. Adiguzel R, Esener H, Ergin Z, Aktan E, Turan N and Sekerci M. Synthesis and Characterization of Novel Ni(II), Cu(II) and Cd(II) Complexes of 4-(2-Chlorphenylazo)-1H-pyrazole-3,5-diamine. Asian J. Chem. 2011; 23:1846-1850.
  22. Esener H, Adiguzel R, Ergin Z, Aktan E, Turan N and Sekerci M. Synthesis and Characterization of Novel Mn(II), Co(III), Ni(II) and Cd(II) Complexes from 4-(2-nitrophenylazo)-1H-pryazole-3,5-diamine. Advanced Sci. Let. 2011; 4:3669-3675.
  23. Muradian KK, Utko NA, Fraifeld V, Mozzhukhine TG, Pishel IN and Litoshenko AY. Superoxide dismutase, Catalase and glutathione peroxidase activities in the liver of young and old mice: linear regression and correlation. Arch Gerontol. Geriatr. 2002; 35:205-214.
  24. Sinha AK. Colorimetry assay of catalase. Anal.Biochem. 1972; 47:380-394.
  25. Alexander NM. A spectrophotometric assay for iodide oxidation by thyroid peroxidase. Anal Biochem. 1962; 4:341-345.
  26. Fraga CG, Leibovitz BE and Tappel AL. Lipid peroxidation measured as Thiobarbituric acid reactive substance in tissue slices: characterization and comparision with homogenates and microsomes. Free Radic. Biol. Med. 1988; 4:155-161.
  27. Karatas F, Bal C, Kara H, Yılmaz I and Cukurovali A. The Effect of Thiosemicarbazone Derivatives on Some Blood Parameters in Rats. J. Health Sci. 2009; 18:93-99.
  28. Yusef JM and Sadik AA. Studies the effected microbial, biochemical analysis and histological examination of pendant coumarin thiocarbohydrazone and its cobalt (II) complex in rats, Global Journal of Molecular Sci. 2012; 5:18-29.
  29. Thomas J and Parameswaran G. Structural, thermoanalytical and antitumour studies of metal chelates of anthracene-9 carboxaldehyde thiosemicarbazoneAsian J. Chem. 2002; 14:1354-1364.
  30. Murthy N and Dharmarajan TS. Synthesis, characterization and biological activity of copper (II) complexes with phenylglyoxal bis-(Thiosemicarbazones). Asian J. Chem. 2002; 14:1325-1330.
  31. Sari A and Cukurovali A. MDA Effect of the Thiosemicarbazone Derivative Schiff Base 1-(1-mesityl-1-methylcyclobutane-3-yl)-2-suksinimido Etanon Thiosemicarbazone in Rabbits, 2012 International Conference on Bioscience, International Conference on Bioscience, Biochemistry and Bioinformatics Singapoore, 2012.
  32. McCord JM and Fridovich, I. Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein) J. Biol. Chem. 1969; 244: 6049-6055.
  33. Mates J M, Porez-Gomez C nad Nunez de Castro I. Antioxidant Enzymes and Human Diseases. Clin. Biochem. 1999; 32:595-603.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 841 Download : 341

Most read articles by the same author(s)