Proteasomes reactivator REG gamma enchances oncogenicity of mda-mb-231 cell line via promoting cell proliferation and inhibiting apoptosis
Corresponding Author(s) : M. Tian
Cellular and Molecular Biology,
Vol. 55 No. 4: General Papers
To investigate the effect of proteasomes reactivator REG gamma (Î³) on cell cycle and apoptosis in vitro and in vivo. In vitro, we first constructed recombinant plasmid of PcDNA3.1-REGÎ³ and then transfected REGÎ³ into MDA-MB-231 cell line. We confirmed the transfection efficiency by Western blot. Subsequently, we observed cell growth, cycle and colony formation. Specific proliferative molecule proliferating cell nuclear antigen (PCNA) and apoptosis related signal molecule Caspase-3 was assayed by immmunohistochemistry and absorption spectrometry, respectively. In vivo, we successfully established transplantation tumor nude mice model. We determined REGÎ³ mRNA level in the transplantation tumor tissue. Then, FCM was used to determine cell cycle, apoptosis and CD16. Finally, we employed immunohistochemistry to determine P21 positive expression. However, the cells transfected with REGÎ³ grew more rapidly compared with non-transfected ones. Increased cells were observed in S+G2+M phase and S phase in the REGÎ³ transfected group. PCNA expression level in the transfected cells was higher than that in non-transfected ones. In vivo, we observed the similar phenomenon including more rapid tumor growth, higher REGÎ³ mRNA expression, decreased cells number in G0/G1 phase and G2/M phase, increased cells in S phase and decreased apoptosis in the transfected group. In the study of related molecules, we also found related molecules P21 and CD16 positive expression rate were obviously lower than non-infected ones. In present study, we found oncogenicity of MDA-MB-231 cell transfected with REGÎ³ was enhanced, which might be realized via REGÎ³ promoting cell growth, inhibiting cell apoptosis, degrading P21 and suppressing activation of NK, suggesting REGÎ³ promoting tumor growth is a process involving multiple factor mechanisms.
Proteasomes reactivator REG gamma cell cycle proliferation apoptosis.
Tian, M., Xiaoyi, W., Xiaotao, L., & Guosheng, R. (2009). Proteasomes reactivator REG gamma enchances oncogenicity of mda-mb-231 cell line via promoting cell proliferation and inhibiting apoptosis. Cellular and Molecular Biology, 55(4), 1121–31. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/1054
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