Rapid detection of listeria monocytogenes in food by polymerase chain reaction
Corresponding Author(s) : H Ennaji
Cellular and Molecular Biology,
Vol. 55 No. 4: General Papers
The standard conventional methods for the detection of Listeria monocytogenes in foods require high time 7 to 10 days to give ready results. To dissolve this problem we have evaluate a short method using Polymerase Chain Reaction (PCR) to analyze food samples. In parallel with this study, a comparison was made between PCR amplification from templates directly prepared from food and the official standard ISO procedure 11290-1. In this study we have used a Half Frazer broth as an enrichment medium; there were positive results of PCR detection of L. monocytogenes in different food sample analyzed (milk, cheese and meat) with approximately 1.5 101 Colony Forming Units /25g in less than 36 h. This PCR procedure has proved to be rapid and sensitive method suitable for the routine analysis; firstly, because this assay required just a short pre-enrichment step before PCR. Secondly, this procedure is very simple and time-saving; it could take less than one working day to obtain results if initial microbiological load was very important.
Cheese Listeria monocytogenes meats milk Polymerase Chain Reaction.
Ennaji, H., Timinouni, M., Ennaji, M. M., Ait M‘hand, R., Hassar, M., & Cohen, N. (2009). Rapid detection of listeria monocytogenes in food by polymerase chain reaction. Cellular and Molecular Biology, 55(4), 1104–10. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/1052
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