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Vol. 62 No. 6: Issue 6

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Human amniotic fluid derived mesenchymal stem cells cause an anti-cancer effect on breast cancer cell line in vitro

M Ghafarzadeh
Lorestan University of Medical sciences Assalian Hospital, Center for Obstetrics and Gynecology khoramabad Iran
A Eatemadi
Tehran University of Medical sciences Department of Medical Biotechnology, School of advance Science in Medicine Tehran Iran
Z Fakhravar
Lorestan University of Medical sciences Razi Drug applied research center khoramabad Iran

Corresponding Author(s) : M Ghafarzadeh

m.ghafarzadeh95@gmail.com

Cellular and Molecular Biology, Vol. 62 No. 6: Issue 6

Abstract

Human amniotic fluid stem cells (hAFSCs) have the ability to self-renew, and multipotent differentiation into three germ layer cells. We obtained 5 ml amniotic fluid from ten 16-20 week pregnant women undergoing amniocentesis. hAFSCs were isolated from all samples, co-cultured with T47D breast cancer cell line and characterized using flow cytometry and RT-PCR. After 3, 4 and 5 days, T47D and HSFCs viability were evaluated with MTT assay. After 5 days of co-culture T47D cells viability were decreased. Our findings showed that hAFSCs can release soluble factors in cell culture, causing an efficient anticancer effect.

Keywords

Stem cell T47D co-culture anticancer therapy.
Ghafarzadeh, M., Eatemadi, A., & Fakhravar, Z. (2016). Human amniotic fluid derived mesenchymal stem cells cause an anti-cancer effect on breast cancer cell line in vitro. Cellular and Molecular Biology, 62(6), 102–106. Retrieved from http://cellmolbiol.org/index.php/CMB/article/view/881
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