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Copyright (c) 2024 Rahim Ullah, Farooq Ali, Qismat Shakeela, Shakira Ghazanfar, Johar Jamil, Tariq Aziz, Ayaz Ali Khan, Abdullah F. Alasmari, Shehzad Ahmed
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Growth optimization, antibiogram, and molecular identification of Bacillus species isolated from the human gut
Corresponding Author(s) : Qismat Shakeela
Cellular and Molecular Biology,
Vol. 70 No. 10: Issue 10
Abstract
The human microbial flora is quite diverse and versatile, playing several beneficial roles in association with the host and deriving nutrition from it. The present study aimed to identify gut microbial flora with potential probiotic activities. Eighteen bacterial isolates were screened from ten male individuals in this study. Seven bacterial isolates, NCCP-2046, NCCP-2031, NCCP-2035, NCCP-2040, NCCP-2041, NCCP-2044, and NCCP-2046, were isolated from the gut samples of volunteer men belonging to various areas of Rawalpindi and Islamabad. These bacterial isolates were cultured on De Man Rogosa and Sharpe Media (MRS), Tryptone Soya Agar (TSA), and Nutrient agar, which showed efficient bacterial growth. The morphological and biochemical characteristics of these bacterial strains were studied under their optimal growth conditions, along with molecular investigations. The antibiotic sensitivity pattern was tested using Kirby-Bauer method, which verified the higher MIC against all eight antibiotics used except for oxacillin. Phylogenetic analysis of only four bacterial isolates was performed based on their 16S rRNA sequences, and their top-hit sequence similarities in NCBI and EzBioCloud.net (95-98% and 94%) verified that these bacterial candidates belong to the Priestia and Staphylococcus genera. Based on molecular evidence through phylogeny and sequence similarities with previously defined bacterial candidates, the bacterial strains MG-461621 (NCCP-2031), MG-461622 (NCCP-2035), and MG-561934 (NCCP-2046) are presumed to be members of Priestia or novel species/genera, while MG-461623 (NCCP-2039) is also found to be a previously identified species of Staphylococcus. However, due to decreased similarity with the top-hit sequences, it could also be presumed to represent a member of a novel genus.
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