The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
G-protein coupled receptor 120 is involved in glucose metabolism in fat cells.
Corresponding Author(s) : X. Liu
liuathyd@163.com
Cellular and Molecular Biology,
Vol. 58 No. 2: General Papers
Abstract
Free fatty acids (FFA) are closely related to insulin resistance in which proteins such as insulin receptor substrate 1 (IRS-1), phosphatidylinositol 3 kinase (PI3K) and glucose transporter type 4 (GLUT4) are involved. Recent researches have shown that G-protein coupled receptor 120 (GPR120) is a receptor for medium and long chain FFA. This study aimed to evaluate the relationship between GPR120 and proteins related to the glucose metabolism. We used siRNA technique to down-regulate the GPR120 expression in 3T3-L1 cells before incubation with palmitic acid (PA), and evaluated the effect of GPR120 expression on the levels of IRS-1, PI3K and GLUT4 after PA induction. RT-PCR and western blot were performed to detect gene and protein levels in differentiated cells. RT-PCR result showed that GPR120 mRNA significantly increased in differentiated cells. GPR120-SiRNA transfection significantly down-regulated GPR120 gene and protein level in differentiated 3T3-L1 cells on day 5 after PA-induced and also decreased lipid droplets accumulated within the cells. SiRNA-mediated decreased of GRP120 was associated with significant reductions in gene and protein levels of IRS-1 and GLUT4. Our results showed that the expression level of GPR120 affected the expression of proteins related to the glucose metabolism, suggesting a contribution of GPR120 in the insulin resistance.
Keywords
Free fatty acids
insulin resistance
G-protein coupled receptor 120
GPR120
glucose metabolism.
Liu, D., Wang, L., Meng, Q., Kuang, H., & Liu, X. (2012). G-protein coupled receptor 120 is involved in glucose metabolism in fat cells. Cellular and Molecular Biology, 58(2), 1757–62. Retrieved from http://cellmolbiol.org/index.php/CMB/article/view/561
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX