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Copyright (c) 2023 Guoqiang Jing, Shuying Yin
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Expression and Related Mechanism of miR-744 in Ischemia-reperfusion Rat Model
Corresponding Author(s) : Shuying Yin
Cellular and Molecular Biology,
Vol. 69 No. 8: Issue 8
Abstract
To study the expression of miR-744 in the rat model of ischemia-reperfusion (I-R) and its related mechanism. Seventy-five Wistar rats were randomly divided into 3 groups: sham operation group (SOG), model group (MG) and miR-744 group, with 25 in each group. The expression levels of IL-1β, IL-6 and TNF-α were observed by Western Blot after the model preparation, while miR-744 expression was detected by reverse transcription polymerase chain reaction (RT-PCR). The cerebral infarction volume of rats in the MG was significantly larger than that in the miR-744 group (P<0.05). The MG exhibited a markedly higher brain tissue water content than the SOG and the miR-744 group (P<0.05). When compared within the latter two groups, the brain tissue water content in the SOG was significantly lower than that in the miR-744 group (P<0.05). As to miR-744 expression, the relative expression of miR-744 in the brain tissue of the MG was the lowest among the three groups. When compared within the remaining two groups, the miR-744 expression of the miR-744 group was remarkably higher than that of the SOG (P<0.05). In terms of the expression levels of inflammatory factors, the expressions of IL-1β, IL-6 and TNF-α in the brain tissue of the SOG and the miR-744 group were significantly lower than those in the MG, and those of the SOG were significantly lower than that of the miR-744 group. MiR-744 may be involved in the development and progression of I-R in rats, and its mechanism may be related to the regulation of inflammatory response.
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