Qingchuan Ma; Junfang  Xue; Xiqin  Xin; Ni  Mu; Xi  Wu; Bing  Yang; Na  Xu; Juan  Du

doi:10.14715/cmb/2022.68.3.45

Issue

Vol. 68 No. 3: New findings of cellular, molecular, and medical biology using nanotechnology

Issue Published : August 9, 2022

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Different Detection Methods of Blood Nucleic Acid and Enzyme-linked Immunosorbent Assay for Blood Virus Screening

Detection Methods of Blood Nucleic Acid and ELISA

https://doi.org/10.14715/cmb/2022.68.3.45

Qingchuan Ma

Department of Clinical Laboratory, Laboratory First People’s Hospital of Tianshui, Tianshui, 741000, China

Junfang Xue

Operation Room, People's Hospital of Gaomi, Gaomi, 261500, China

Xiqin Xin

Department of Clinical Laboratory, Tianshui maternal and Child Health Hospital, Tianshui, 741000, China

Ni Mu

Department of Pathology, Laboratory First People’s Hospital of TianShui, Tianshui, 741000, China

Xi Wu

Department of Clinical Laboratory, Laboratory First People’s Hospital of Tianshui, Tianshui, 741000, China

Bing Yang

Department of Clinical Laboratory, Laboratory First People’s Hospital of Tianshui, Tianshui, 741000, China

Na Xu

Department of Clinical Laboratory, Laboratory First People’s Hospital of Tianshui, Tianshui, 741000, China

Juan Du

Department of Clinical Laboratory, Laboratory First People’s Hospital of Tianshui, Tianshui, 741000, China

Corresponding Author(s) : Xiqin Xin

xxq18093894995@163.com

Cellular and Molecular Biology, Vol. 68 No. 3: New findings of cellular, molecular, and medical biology using nanotechnology
Article Published : March 31, 2022

Abstract

Through the detection of antigens or antibodies of related viruses in blood, the incidence of transfusion-transmitted diseases can be reduced, the comprehensive performance indicators of the human body can be judged, and the disease can be effectively treated and prevented. The purpose of this study is to analyze the screening results of blood viruses by different detection methods of blood nucleic acid and enzyme-linked immunosorbent assay. In this study, the comparison and data analysis of the two detection methods in the study were carried out through the comparison method and data analysis method, and the functional analysis is combined with the detection principle. The positive rate of anti-HCV was 1.67% (10 / 600) by ELISA and 0.34% (2 / 590) by nucleic acid. Conclusion enzyme immunoassay is not sensitive to the antigen-antibody reaction in the window stage, and there is a mistake in the detection. However, nucleic acid detection has high sensitivity because of its PCR principle, but it is also prone to false-positive.

Keywords

blood virus detection nucleic acid detection enzyme-linked immunosorbent assay blood virus screening

Ma, Q., Xue, J. ., Xin, X. ., Mu, N. ., Wu, X. ., Yang, B. ., Xu, N. ., & Du, J. . (2022). Different Detection Methods of Blood Nucleic Acid and Enzyme-linked Immunosorbent Assay for Blood Virus Screening: Detection Methods of Blood Nucleic Acid and ELISA. Cellular and Molecular Biology, 68(3), 411–417. https://doi.org/10.14715/cmb/2022.68.3.45