Issue

Vol. 65 No. 5: Issue 5

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Naringin inhibits lipopolysaccharide-induced activation of microglia cells

https://doi.org/10.14715/cmb/2019.65.5.6
Jie Bai
Department of Anesthesiology, the Second Affiliated Hospital of Xi'an Jiaotong University, 710004, Xi'an, Shaanxi, China
Siyuan Li
Department of Anesthesiology, the Second Affiliated Hospital of Xi'an Jiaotong University, 710004, Xi'an, Shaanxi, China
Gang Wu
Department of Anesthesiology, the Second Affiliated Hospital of Xi'an Jiaotong University, 710004, Xi'an, Shaanxi, China
Lei Ma
Department of Anesthesiology, the Second Affiliated Hospital of Xi'an Jiaotong University, 710004, Xi'an, Shaanxi, China
Chen Li
Department of Geriatrics Neurology, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, 710004, Xi'an, Shaanxi, China
Hongtao Liu
Department of Anesthesiology, the Second Affiliated Hospital of Xi'an Jiaotong University, 710004, Xi'an, Shaanxi, China
Pengbo Zhang
Department of Anesthesiology, the Second Affiliated Hospital of Xi'an Jiaotong University, 710004, Xi'an, Shaanxi, China

Corresponding Author(s) : Pengbo Zhang

tfmg21@163.com

Cellular and Molecular Biology, Vol. 65 No. 5: Issue 5

Abstract

The purpose of this study was to investigate the effect of naringin on lipopolysaccharide (LPS)-induced activation of BV2 microglia and inflammatory factor release, and the mechanism involved. Different concentrations of naringin were used to pretreat BV2 cells for 30 min, after which they were stimulated with 100 ng/mL LPS for different durations. The levels of NO, IL-1β and TNF-α in the cell culture medium was determined with ELISA and Griess method. The mRNA expressions of IL-1β and TNF-α was determined with RT-PCR. Changes in ERK and p65/NF-κB signaling pathway proteins were assayed with Western blotting. After 12 h stimulation of BV2 cells with LPS, the levels of IL-1β and TNF-α in the cell culture medium were significantly increased, but naringin had no significant effect on these inflammatory factors. In the cells pretreated with naringin, LPS stimulated the activation of microglia to produce IL-1β and TNF-α in a dose-dependent manner. Naringin inhibited LPS-induced release of IL-1β, to a certain extent. The TNF-α gene was overexpressed. In addition, LPS stimulated a dose-dependent decrease in NO production by BV2 after pretreatment with different concentrations of naringin. Naringin pretreatment of cells significantly inhibited the activation of p65/NF-κB in a concentration-dependent manner. In BV2 microglia, naringin inhibits LPO-induced production of NO and inflammatory factors, through a mechanism involving inhibition of activation of the p65/NF-κB signaling pathway.

Keywords

Naringin Lipopolysaccharide Inflammatory factors BV-2 cells.
Bai, J., Li, S., Wu, G., Ma, L., Li, C., Liu, H., & Zhang, P. (2019). Naringin inhibits lipopolysaccharide-induced activation of microglia cells. Cellular and Molecular Biology, 65(5), 38–42. https://doi.org/10.14715/cmb/2019.65.5.6
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Möller JC, Klein MA, Haas S, Jones LL, Kreutzberg GW, Raivich G. Regulation of thrombospondin in the regenerating mouse facial motor nucleus. Glia 2015; 17: 121-132.
  2. Ballarin L, Cammarata M. Lessons in immunity : from single-cell organisms to mammals. 308.
  3. Suzumura A, Ikenaka K. Neuron-glia interaction in neuroinflammation. Adv Neurobiol 2013; 14: 1.
  4. Wyss-Coray T, Mucke L. Inflammation in neurodegenerative disease--a double-edged sword. Neuron 2002; 35: 419-432.
  5. Kozlov SV. Inflammation and cancer. Methods and protocols. Volume 1: Experimental models and practical approaches. Preface. Methods Mol Biol 2009; 511: 5-9.
  6. Affò S, Dominguez M, Lozano JJ, Sancho-Bru P, Rodrigo-Torres D, Morales-Ibanez O, et al. Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis. Gut 2013; 62: 452-460.
  7. Turner CW. Vitamins and hormones. Advances in research and applications. Vol. XIV: Edited by Robert S. Harris, G. F. Marrian and Kenneth V. Thimann. Academic Press Inc. New York, N. Y. 1956. xi + 486 pp. Price $10.00. Arch Biochem Biophys 2013; 70: 303.
  8. Peter I, Dubinsky M, Bressman S, Park A, Lu C, Chen N, et al. Anti-Tumor Necrosis Factor Therapy and Incidence of Parkinson Disease Among Patients With Inflammatory Bowel Disease. JAMA Neurol 2018; 75: 939-946.
  9. Burguillos MA, Hajji N, Englund E, Persson A, Cenci AM, Machado A, et al. Apoptosis-inducing factor mediates dopaminergic cell death in response to LPS-induced inflammatory stimulus: evidence in Parkinson's disease patients. Neurobiol Dis 2011; 41: 177-188.
  10. Wenzel U, Schoberl K, Lohner K, Daniel H. Activation of mitochondrial lactate uptake by flavone induces apoptosis in human colon cancer cells. J Cell Physiol 2005; 202: 379-390.
  11. Sari E, Berczynski P, Kladna A, Kruk I, Dundar OB, Szymanska M, et al. Synthesis and in vitro Antioxidant Activity Study of Some Novel Substituted Piperazinyl Flavone Compounds. Med Chem 2018; 14: 372-386.
  12. D'Abrosca B, Pacifico S, Scognamiglio M, D'Angelo G, Galasso S, Monaco P, et al., A new acylated flavone glycoside with antioxidant and radical scavenging activities from Teucrium polium leaves. Nat Prod Res 2013; 27: 356-363.
  13. Wei K, Xie Y, Chen T, Fu B, Cui S, Wang Y, et al. ERK1/2 signaling mediated naringin-induced osteogenic differentiation of immortalized human periodontal ligament stem cells. Biochem Biophys Res Commun 2017; 489: 319-325.
  14. Lv Z, Wu W, Ge S, Jia R, Lin T, Yuan Y, et al. Naringin protects against perfluorooctane sulfonate-induced liver injury by modulating NRF2 and NF-kappaB in mice. Int Immunopharmacol 2018; 65: 140-147.
  15. Zhang YS, Li Y, Wang Y, Sun SY, Jiang T, Li C, et al., Naringin, a natural dietary compound, prevents intestinal tumorigenesis in Apc (Min/+) mouse model. J Cancer Res Clin Oncol 2016; 142: 913-925.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 846 Download : 457

Most read articles by the same author(s)