A new target for the treatment of endometrium cancer by succinic acid

Elif Sinem Iplik; Tolga Catmakas; Bedia Cakmakoglu

doi:10.14715/cmb/2018.64.1.11

Issue

Vol. 64 No. 1: Issue 1

Issue Published : February 7, 2018

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A new target for the treatment of endometrium cancer by succinic acid

https://doi.org/10.14715/cmb/2018.64.1.11

Elif Sinem Iplik

Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, Istanbul, Turkey

Tolga Catmakas

Department of Molecular Medicine, Aziz Sancar Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey

Bedia Cakmakoglu

Department of Molecular Medicine, Aziz Sancar Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey

Corresponding Author(s) : Bedia Cakmakoglu

bedia@istanbul.edu.tr

Cellular and Molecular Biology, Vol. 64 No. 1: Issue 1
Article Published : January 31, 2018

Abstract

Endometrium cancer is the most common invasive gynecologic malignancy in developed countries. Succinic acid (CO2HCH2-CH2CO2H) is a type of dibasic acid that has uncolored crystal. Succinic acid is used in bakery products and aromatized products. It is naturally found in some vegetables. Succinic acid has no adverse effects because it is metabolized by body cells and has a role in the tricarboxylic acid cycle (TCA) as a cycle media component. The TCA cycle and its enzyme components have some crucial roles for basal cell metabolism. Any mistakes, concentration differences in product, or enzyme deficiencies are important within the cell this cycle. In this proposal project, we aimed to investigate the effect of succinic acid at different doses and at different times in an endometrial cancer cell line. The study was performed using methods that determine for apoptosis (for cytotoxicity, WST-1, for caspase enzyme activity, Caspase 3/BCA; apoptotic determination using flow cytometry; Annexin V; to understand mitochondrial membrane potential; JC-1). The results showed that 5 and 10 µM concentration of succinic acid resulted in apoptosis in endometrium cancer; no such effect was seen in the control cell line, which comprised healthy lung cells. According to our results, it is thought that succinic acid would be effective for the treatment of endometrial cancer cell lines, thus providing new data for other areas of cancer research.

Keywords

Endometrial cancer Succinic acid Anticancer.

Iplik, E. S., Catmakas, T., & Cakmakoglu, B. (2018). A new target for the treatment of endometrium cancer by succinic acid. Cellular and Molecular Biology, 64(1), 60–63. https://doi.org/10.14715/cmb/2018.64.1.11

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References

Macmahon B. Risk factors for endometrial cancer. Gynecologic Oncology 1974; 2:122.
Modugno F, Ness RB, Chen C, Weiss NS. Inflammation and endometrial cancer: a hypothesis. Cancer epidemiology, biomarkers & prevention 2005; 14:2840-2847.
Lan L, Tong-Xun L, Mou-Ming Z, Chun C, Bo-En Y, Sheng T, Fei Y. Functional, nutritional and conformational changes from deamidation of wheat gluten with succinic acid and citric acid. Food Chemistry 2010; 123:123-130.
Vinay BJ, Sven C, Bert L, Kevin JV, Jan AD, Christophe MC. Mapping of Saccharomyces cerevisiae metabolites in fermenting wheat straight-dough reveals succinic acid as pH-determining factor. Food Chemistry 2013; 136:301–308.
Yong-lan X, Ke-quan C, Wen-yu D, Jiang-feng M, Min Z, Min J, Ping W, Ping-Kai O. Succinic acid production by Actinobacillus succinogenes NJ113 using corn steep liquor powder as nitrogen source, Bioresource Technology 2013; 136:775-779.
Akira T, Sanae O, Pascal H, Eiichi S. Metabolic engineering of Lactobacillus plantarum for succinic acid production through activation of the reductive branch of the tricarboxylic acid cycle. Enzyme and Microbial Technology 2013; 53:97-103.
Bellamy COC, Malcomson RDG, Harrison DJ, Wyllie AH. Cell death in health and disease: the biology and regulation of apoptosis. Seminar in Cancer Biology 1995; 6:3-16.
Ellis RE, Yuan J, Horvitz HR. Mechanisms and functions of cell death. Annual Review of Cell and Developmental Biology 1991; 7:663-698.
Evan G, Littlewood T. A matter of life and cell death. Science 1998; 281:1317-1321.
Seyfried TN, Flores R, Poff AM, D'Agostino DP. Cancer as a metabolic disease: implications for novel therapeutics. Carcinogenesis 2009; 14:625–635.
Tyler D. The Mitochondrion in Health and Diseases. New York: Weinheim; 557; 1992.
Whelan DT, Hill RE, McClorry S. Fumaric aciduria: a new organic aciduria, associated with mental retardation and speech impairment. Clinica Chimica Acta 1983; 132:301–308.
Habano W, Sugai T, Nakamura S, Uesugi N, Higuchi T, Terashima M, Horiuchi S. Reduced expression and loss of heterozygosity activation of the of the SDHD gene in colorectal and gastric cancer. Oncology Report 2003; 10:1375–1380.
Frederiksen CM, Knudsen S, Laurberg S, Orntoft TF. Classification of Dukes' B and C colorectal cancers using expression arrays. Journal of Cancer Research and Clinical Oncology 2003; 129:263–271.

References

Macmahon B. Risk factors for endometrial cancer. Gynecologic Oncology 1974; 2:122.

Modugno F, Ness RB, Chen C, Weiss NS. Inflammation and endometrial cancer: a hypothesis. Cancer epidemiology, biomarkers & prevention 2005; 14:2840-2847.

Lan L, Tong-Xun L, Mou-Ming Z, Chun C, Bo-En Y, Sheng T, Fei Y. Functional, nutritional and conformational changes from deamidation of wheat gluten with succinic acid and citric acid. Food Chemistry 2010; 123:123-130.

Vinay BJ, Sven C, Bert L, Kevin JV, Jan AD, Christophe MC. Mapping of Saccharomyces cerevisiae metabolites in fermenting wheat straight-dough reveals succinic acid as pH-determining factor. Food Chemistry 2013; 136:301–308.

Yong-lan X, Ke-quan C, Wen-yu D, Jiang-feng M, Min Z, Min J, Ping W, Ping-Kai O. Succinic acid production by Actinobacillus succinogenes NJ113 using corn steep liquor powder as nitrogen source, Bioresource Technology 2013; 136:775-779.

Akira T, Sanae O, Pascal H, Eiichi S. Metabolic engineering of Lactobacillus plantarum for succinic acid production through activation of the reductive branch of the tricarboxylic acid cycle. Enzyme and Microbial Technology 2013; 53:97-103.

Bellamy COC, Malcomson RDG, Harrison DJ, Wyllie AH. Cell death in health and disease: the biology and regulation of apoptosis. Seminar in Cancer Biology 1995; 6:3-16.

Ellis RE, Yuan J, Horvitz HR. Mechanisms and functions of cell death. Annual Review of Cell and Developmental Biology 1991; 7:663-698.

Evan G, Littlewood T. A matter of life and cell death. Science 1998; 281:1317-1321.

Seyfried TN, Flores R, Poff AM, D'Agostino DP. Cancer as a metabolic disease: implications for novel therapeutics. Carcinogenesis 2009; 14:625–635.

Tyler D. The Mitochondrion in Health and Diseases. New York: Weinheim; 557; 1992.

Whelan DT, Hill RE, McClorry S. Fumaric aciduria: a new organic aciduria, associated with mental retardation and speech impairment. Clinica Chimica Acta 1983; 132:301–308.

Habano W, Sugai T, Nakamura S, Uesugi N, Higuchi T, Terashima M, Horiuchi S. Reduced expression and loss of heterozygosity activation of the of the SDHD gene in colorectal and gastric cancer. Oncology Report 2003; 10:1375–1380.

Frederiksen CM, Knudsen S, Laurberg S, Orntoft TF. Classification of Dukes' B and C colorectal cancers using expression arrays. Journal of Cancer Research and Clinical Oncology 2003; 129:263–271.

Author biographies is not available.