CCAT1 expressed in malignant and pre-malignant human gastric tissues

X. Li; Y. Zhou; H. Qian

doi:10.14715/cmb/2017.63.5.16

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Vol. 63 No. 5: Issue 5

Issue Published : July 9, 2017

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CCAT1 expressed in malignant and pre-malignant human gastric tissues

https://doi.org/10.14715/cmb/2017.63.5.16

X. Li

Department of General Surgery, The first Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China

Y. Zhou

Department of General Surgery, The second Affiliated Hospital of NanjingMedical University, Wuxi, Jiangsu, China

H. Qian

Department of General Surgery, The first Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China

Corresponding Author(s) : H. Qian

qianhaixin1@163.com

Cellular and Molecular Biology, Vol. 63 No. 5: Issue 5
Article Published : May 20, 2017

Abstract

CCAT1 has been reported to implicate in various human malignancies. However, its expression in the oncogenesis of gastric cancer (GC) remains unknown. Quantitative reverse transcription (RT)-qPCR assay was performed to detect the expression of CCAT1 in 68cases of GC tissues,paired tumor-proximal gastric mucosa and normal gastric mucosa, 19 cases of gastric intraepithelial neoplasia (GIN), and the sera of these 68 GC patients and 22 healthy volunteers. And the relationship between CCAT1 and clinicopathologic features of GC patients were analyzed. Inthe present study, we found that CCAT1 expression was significantly abnormally deregulated in GC tissues (185.43 ± 21.37), GIN tissues (121.30 ± 43.61), tumor-proximal mucosa (8.9 ± 1.21), and normal mucosa (1.5 ± 0.55). CCAT1 wasalso overexpressed in the sera of GC patients (47.40 ± 6.60) compared with healthy controls (0.62 ± 0.06; p < 0.001). And high CCAT1 expression in GC tissues was associated with high tumor burden, including larger tumor size, lymphatic metastasis and advanced TNM stage (all p < 0.05). These results reveal that CCAT1 is a detectable biomarkerforgastric cancertumorigenesis and may be utilized as adiagnosticand prognosticindicator.

Keywords

CCAT1 Gastric cancer Expression Tissue sera.

Li, X., Zhou, Y., & Qian, H. (2017). CCAT1 expressed in malignant and pre-malignant human gastric tissues. Cellular and Molecular Biology, 63(5), 89–93. https://doi.org/10.14715/cmb/2017.63.5.16

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References

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, et al. Cancer statistics in China, 2015. CA Cancer J Clin 2016.
Lauwers GY CF, Graham DY, Curado MP Tumours of the stomach. In: Bosman FT CF, Hruban RH, Theise ND, editor. WHO Classification of Tumours of the Digestive System
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Orditura M, Galizia G, Sforza V, Gambardella V, Fabozzi A, Laterza MM, et al. Treatment of gastric cancer. World J Gastroenterol 2014;20:1635-49.
Xiang JF, Yin QF, Chen T, Zhang Y, Zhang XO, Wu Z, et al. Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus. Cell Res 2014;24:513-31.
Yang F, Xue X, Bi J, Zheng L, Zhi K, Gu Y, et al. Long noncoding RNA CCAT1, which could be activated by c-Myc, promotes the progression of gastric carcinoma. J Cancer Res Clin Oncol 2013;139:437-45.
Nissan A, Stojadinovic A, Mitrani-Rosenbaum S, Halle D, Grinbaum R, Roistacher M, et al. Colon cancer associated transcript-1: a novel RNA expressed in malignant and pre-malignant human tissues. Int J Cancer 2012;130:1598-606.
Alaiyan B, Ilyayev N, Stojadinovic A, Izadjoo M, Roistacher M, Pavlov V, et al. Differential expression of colon cancer associated transcript1 (CCAT1) along the colonic adenoma-carcinoma sequence. BMC Cancer 2013;13:196.
Deng L, Yang SB, Xu FF, Zhang JH. Long noncoding RNA CCAT1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge. J Exp Clin Cancer Res 2015;34:18.
Ma MZ, Chu BF, Zhang Y, Weng MZ, Qin YY, Gong W, et al. Long non-coding RNA CCAT1 promotes gallbladder cancer development via negative modulation of miRNA-218-5p. Cell Death Dis 2015;6:e1583.
Washington K. 7th edition of the AJCC cancer staging manual: stomach. Ann Surg Oncol 2010;17:3077-9.
Dong L, Qi P, Xu MD, Ni SJ, Huang D, Xu QH, et al. Circulating CUDR, LSINCT-5 and PTENP1 long noncoding RNAs in sera distinguish patients with gastric cancer from healthy controls. Int J Cancer 2015.
Gan L, Xu M, Zhang Y, Zhang X, Guo W. Focusing on long noncoding RNA dysregulation in gastric cancer. Tumour Biol 2015;36:129-41.
Xin Y, Li Z, Shen J, Chan MT, Wu WK. CCAT1: a pivotal oncogenic long non-coding RNA in human cancers. Cell Prolif 2016;49:255-60.
Guo X, Hua Y. CCAT1: an oncogenic long noncoding RNA in human cancers. J Cancer Res Clin Oncol 2016.
Mizrahi I, Mazeh H, Grinbaum R, Beglaibter N, Wilschanski M, Pavlov V, et al. Colon Cancer Associated Transcript-1 (CCAT1) Expression in Adenocarcinoma of the Stomach. J Cancer 2015;6:105-10.
Zhou B, Wang Y, Jiang J, Jiang H, Song J, Han T, et al. The long noncoding RNA colon cancer-associated transcript-1/miR-490 axis regulates gastric cancer cell migration by targeting hnRNPA1. IUBMB Life 2016;68:201-10.
Zhu HQ, Zhou X, Chang H, Li HG, Liu FF, Ma CQ, et al. Aberrant Expression of CCAT1 Regulated by c-Myc Predicts the Prognosis of Hepatocellular Carcinoma. Asian Pac J Cancer Prev 2015;16:5181-5.
Zhang XF, Liu T, Li Y, Li S. Overexpression of long non-coding RNA CCAT1 is a novel biomarker of poor prognosis in patients with breast cancer. Int J Clin Exp Pathol 2015;8:9440-5.
Zhang E, Han L, Yin D, He X, Hong L, Si X, et al. H3K27 acetylation activated-long non-coding RNA CCAT1 affects cell proliferation and migration by regulating SPRY4 and HOXB13 expression in esophageal squamous cell carcinoma. Nucleic Acids Res 2016.
He X, Tan X, Wang X, Jin H, Liu L, Ma L, et al. C-Myc-activated long noncoding RNA CCAT1 promotes colon cancer cell proliferation and invasion. Tumour Biol 2014;35:12181-8.

References

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, et al. Cancer statistics in China, 2015. CA Cancer J Clin 2016.

Lauwers GY CF, Graham DY, Curado MP Tumours of the stomach. In: Bosman FT CF, Hruban RH, Theise ND, editor. WHO Classification of Tumours of the Digestive System

p. 48-59.

Park JY, von Karsa L, Herrero R. Prevention strategies for gastric cancer: a global perspective. Clin Endosc 2014;47:478-89.

Orditura M, Galizia G, Sforza V, Gambardella V, Fabozzi A, Laterza MM, et al. Treatment of gastric cancer. World J Gastroenterol 2014;20:1635-49.

Xiang JF, Yin QF, Chen T, Zhang Y, Zhang XO, Wu Z, et al. Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus. Cell Res 2014;24:513-31.

Yang F, Xue X, Bi J, Zheng L, Zhi K, Gu Y, et al. Long noncoding RNA CCAT1, which could be activated by c-Myc, promotes the progression of gastric carcinoma. J Cancer Res Clin Oncol 2013;139:437-45.

Nissan A, Stojadinovic A, Mitrani-Rosenbaum S, Halle D, Grinbaum R, Roistacher M, et al. Colon cancer associated transcript-1: a novel RNA expressed in malignant and pre-malignant human tissues. Int J Cancer 2012;130:1598-606.

Alaiyan B, Ilyayev N, Stojadinovic A, Izadjoo M, Roistacher M, Pavlov V, et al. Differential expression of colon cancer associated transcript1 (CCAT1) along the colonic adenoma-carcinoma sequence. BMC Cancer 2013;13:196.

Deng L, Yang SB, Xu FF, Zhang JH. Long noncoding RNA CCAT1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge. J Exp Clin Cancer Res 2015;34:18.

Ma MZ, Chu BF, Zhang Y, Weng MZ, Qin YY, Gong W, et al. Long non-coding RNA CCAT1 promotes gallbladder cancer development via negative modulation of miRNA-218-5p. Cell Death Dis 2015;6:e1583.

Washington K. 7th edition of the AJCC cancer staging manual: stomach. Ann Surg Oncol 2010;17:3077-9.

Dong L, Qi P, Xu MD, Ni SJ, Huang D, Xu QH, et al. Circulating CUDR, LSINCT-5 and PTENP1 long noncoding RNAs in sera distinguish patients with gastric cancer from healthy controls. Int J Cancer 2015.

Gan L, Xu M, Zhang Y, Zhang X, Guo W. Focusing on long noncoding RNA dysregulation in gastric cancer. Tumour Biol 2015;36:129-41.

Xin Y, Li Z, Shen J, Chan MT, Wu WK. CCAT1: a pivotal oncogenic long non-coding RNA in human cancers. Cell Prolif 2016;49:255-60.

Guo X, Hua Y. CCAT1: an oncogenic long noncoding RNA in human cancers. J Cancer Res Clin Oncol 2016.

Mizrahi I, Mazeh H, Grinbaum R, Beglaibter N, Wilschanski M, Pavlov V, et al. Colon Cancer Associated Transcript-1 (CCAT1) Expression in Adenocarcinoma of the Stomach. J Cancer 2015;6:105-10.

Zhou B, Wang Y, Jiang J, Jiang H, Song J, Han T, et al. The long noncoding RNA colon cancer-associated transcript-1/miR-490 axis regulates gastric cancer cell migration by targeting hnRNPA1. IUBMB Life 2016;68:201-10.

Zhu HQ, Zhou X, Chang H, Li HG, Liu FF, Ma CQ, et al. Aberrant Expression of CCAT1 Regulated by c-Myc Predicts the Prognosis of Hepatocellular Carcinoma. Asian Pac J Cancer Prev 2015;16:5181-5.

Zhang XF, Liu T, Li Y, Li S. Overexpression of long non-coding RNA CCAT1 is a novel biomarker of poor prognosis in patients with breast cancer. Int J Clin Exp Pathol 2015;8:9440-5.

Zhang E, Han L, Yin D, He X, Hong L, Si X, et al. H3K27 acetylation activated-long non-coding RNA CCAT1 affects cell proliferation and migration by regulating SPRY4 and HOXB13 expression in esophageal squamous cell carcinoma. Nucleic Acids Res 2016.

He X, Tan X, Wang X, Jin H, Liu L, Ma L, et al. C-Myc-activated long noncoding RNA CCAT1 promotes colon cancer cell proliferation and invasion. Tumour Biol 2014;35:12181-8.

Author biographies is not available.