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Vol. 55 No. 2: Porphyrias and associated pathologies. Biochemistry and molecular biology Part 2

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The expression of protoporphyrinogen oxidase IN HUMAN TISSUES

A V Corrigall
Lennox Eales Porphyria Laboratories, MRC/UCT Liver Research Centre Department of Medicine South Africa
J A H Campbell
Lennox Eales Porphyria Laboratories, MRC/UCT Liver Research Centre Department of Medicine South Africa
K Siziba
Lennox Eales Porphyria Laboratories, MRC/UCT Liver Research Centre Department of Medicine South Africa
R E Kirsch
Lennox Eales Porphyria Laboratories, MRC/UCT Liver Research Centre Department of Medicine South Africa
P N Meissner
Lennox Eales Porphyria Laboratories, MRC/UCT Liver Research Centre Department of Medicine South Africa

Corresponding Author(s) : A V Corrigall

Cellular and Molecular Biology, Vol. 55 No. 2: Porphyrias and associated pathologies. Biochemistry and molecular biology Part 2

Abstract

Protoporphyrinogen oxidase is the penultimate enzyme in the haem biosynthetic pathway. In this study, the expression of protoporphyrinogen oxidase in a variety of human organs has been documented by immunohistochemical means at the light microscopy level in order to shed light on its inter- and intra-organ distribution. The expression varied amongst organs and the various cell types within an organ. The pattern of staining generally reflected presumed metabolic functionality and haem demand. Strongest staining was noted in hepatocytes, proximal convoluted tubules of the kidney, serous cells of the peribronchial gland in the lung, parietal cells of the stomach, tips of the villi in the small intestine and interstitial cells of the testis. Our results suggest that there are some significant sites of haem synthesis in addition to the liver and bone marrow, and should be borne in mind in studies related to haem or porphyrin dynamics and flux.

Keywords

Immunohistological localisation protoporphyrinogen oxidase haem biosynthesis porphyria.
Corrigall, A. V., Campbell, J. A. H., Siziba, K., Kirsch, R. E., & Meissner, P. N. (2009). The expression of protoporphyrinogen oxidase IN HUMAN TISSUES. Cellular and Molecular Biology, 55(2), 89–95. Retrieved from http://cellmolbiol.org/index.php/CMB/article/view/1092
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