TY - JOUR AU - Hao, P. S. AU - Yang, C. AU - Yan, X. P. AU - Li, T. H. AU - Zhang, J. AU - Yu, Q. Y. AU - Zhang, X. AU - Zhang, Y. H. PY - 2017/08/30 Y2 - 2024/03/28 TI - Differences in expression of serum protein in patients with psoriasis vulgaris and blood heat syndrome and healthy volunteers JF - Cellular and Molecular Biology JA - Cell Mol Biol (Noisy-le-grand) VL - 63 IS - 8 SE - Original Research Articles DO - 10.14715/cmb/2017.63.8.9 UR - https://cellmolbiol.org/index.php/CMB/article/view/1237 SP - 38-41 AB - <p align="justify">To explore the mechanism of psoriasis vulgaris (PV), serum protein expression profiles between PV patients with blood-heat syndrome and healthy volunteers were detected by isobaric tags for relative and absolute quantitation (iTRAQ). First, sera from 15 PV patients with blood-heat syndrome and 10 healthy volunteers were collected; then, serum proteins were separated and hydrolyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a specific iTRAQ marker enzyme respectively after further purification and protein abundance treatment. Compared with the control group, differentially expressed proteins in PV patients with blood-heat syndrome were identified and analyzed by tandem mass spectrometry. A total of 787 proteins were identified and 718 proteins had a functional annotation with gene ontology (GO) by iTRAQ in the current study. Significant differences (P &lt;0.05) and great differences (P &lt;0.01) were found in 681 proteins and 536 proteins respectively between the patient group and healthy group. ). Different protein expression profiles in serum existed between PV patients with blood-heat syndrome and healthy volunteers; the differences largely involved immune-related proteins and lipoproteins. The proteins specific for PV with blood-heat syndrome deserves further investigation.</p> ER -