Issue
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
Cell-based evaluation of a novel Dictyophora indusiata polysaccharide against oxidative-induced erythrocyte hemolysis
Corresponding Author(s) : W Liao
Cellular and Molecular Biology,
Vol. 62 No. 1: Issue 1
Abstract
The protective effectof a polysaccharide from Dictyophora indusiata(DP1)against oxidative hemolysis was comprehensively evaluated. The 2, 2-azobis (2-amidino-propane) dihydrochloride (AAPH)-induced erythrocyte hemolysis assay showed that DP1 exhibited excellent anti-hemolytic activity(87.4% hemolysis suppression ratio at 20 nmol/mL). Also, the formation of conjugated diene induced by cupric chloride (CuCl2) in plasma was significantly inhibited by DP1. Besides, DP1 could effectively inhibit AAPH-induced overproduction of reactive oxygen species (81.5 % inhibition at 20 nmol/mL) and alleviated the enhancement of intracellular antioxidant enzymes including superoxide dismutase(SOD), glutathione peroxidase (GPX) andcatalase (CAT) activities. Also, the malondialdehyde (MDA) formation caused by oxidative stress was suppressed by 57.0% at DP1 concentration of 20 nmol/mL. Taken together, the possible intracellular antioxidant detoxifying mechanism of DP1 was probably via preserving the activities of the antioxidant enzymes (SOD, GPx and CAT) as well as inhibiting lipid peroxidation, and thus alleviated erythrocytes oxidation and plasma oxidation.
Keywords
Polysaccharide
Dictyophora indusiata
oxidative hemolysis
reactive oxygen species
antioxidant enzymes.
Liao, W., Chen, L., Yu, B., Lei, Z., Wu, X., Yang, J., & Ren, J. (2016). Cell-based evaluation of a novel Dictyophora indusiata polysaccharide against oxidative-induced erythrocyte hemolysis. Cellular and Molecular Biology, 62(1), 38–44. Retrieved from http://cellmolbiol.org/index.php/CMB/article/view/778
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX