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Vol. 64 No. 3: Issue 3

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Association analysis of calpain 10 gene variants/haplotypes with gestational diabetes mellitus among Mexican women

https://doi.org/10.14715/cmb/2018.64.3.13
Anna Gabriela Castro-Martí­nez
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
José Sánchez-Corona
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
Adriana Patricia Vázquez-Vargas
Unidad Médica de Alta Especialidad-Hospital de Gineco-Obstetricia, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
Alejandra Guadalupe Garcí­a-Zapién
Departamento de Farmacobiologí­a, Centro Universitario de Ciencias Exactas e Ingenierí­as, Universidad de Guadalajara. Guadalajara, Jalisco, México
Andres López-Quintero
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
Héctor Javier Villalpando-Velazco
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
Silvia Esperanza Flores-Martí­nez
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, Méxicol
http://orcid.org/0000-0002-0944-6469

Corresponding Author(s) : Silvia Esperanza Flores-Martí­nez

sefloresm@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 3: Issue 3

Abstract

Gestational diabetes mellitus (GDM) is a metabolically complex disease with major genetic determinants. GDM has been associated with insulin resistance and dysfunction of pancreatic beta cells, so the GDM candidate genes are those that encode proteins modulating the function and secretion of insulin, such as that for calpain 10 (CAPN10). This study aimed to assess whether single nucleotide polymorphism (SNP)-43, SNP-44, SNP-63, and the indel-19 variant, and specific haplotypes of the CAPN10 gene were associated with gestational diabetes mellitus. We studied 116 patients with gestational diabetes mellitus and 83 women with normal glucose tolerance. Measurements of anthropometric and biochemical parameters were performed. SNP-43, SNP-44, and SNP-63 were identified by polymerase chain reaction (PCR)-restriction fragment length polymorphisms, while the indel-19 variant was detected by TaqMan qPCR assays.  The allele, genotype, and haplotype frequencies of the four variants did not differ significantly between women with gestational diabetes mellitus and controls. However, in women with gestational diabetes mellitus, glucose levels were significantly higher bearing the 3R/3R genotype than in carriers of the 3R/2R genotype of the indel-19 variant (p = 0.006). In conclusion, the 3R/3R genotype of the indel-19 variant of the CAPN-10 gene influenced increased glucose levels in these Mexican women with gestational diabetes mellitus.

Keywords

CAPN10 variants Gestational diabetes Haplotypes Mexican women.
Castro-Martí­nez, A. G., Sánchez-Corona, J., Vázquez-Vargas, A. P., Garcí­a-Zapién, A. G., López-Quintero, A., Villalpando-Velazco, H. J., & Flores-Martí­nez, S. E. (2018). Association analysis of calpain 10 gene variants/haplotypes with gestational diabetes mellitus among Mexican women. Cellular and Molecular Biology, 64(3), 81–86. https://doi.org/10.14715/cmb/2018.64.3.13
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