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Vol. 64 No. 3: Issue 3

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Association analysis of calpain 10 gene variants/haplotypes with gestational diabetes mellitus among Mexican women

https://doi.org/10.14715/cmb/2018.64.3.13
Anna Gabriela Castro-Martí­nez
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
José Sánchez-Corona
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
Adriana Patricia Vázquez-Vargas
Unidad Médica de Alta Especialidad-Hospital de Gineco-Obstetricia, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
Alejandra Guadalupe Garcí­a-Zapién
Departamento de Farmacobiologí­a, Centro Universitario de Ciencias Exactas e Ingenierí­as, Universidad de Guadalajara. Guadalajara, Jalisco, México
Andres López-Quintero
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
Héctor Javier Villalpando-Velazco
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, México
Silvia Esperanza Flores-Martí­nez
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, Méxicol
http://orcid.org/0000-0002-0944-6469

Corresponding Author(s) : Silvia Esperanza Flores-Martí­nez

sefloresm@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 3: Issue 3

Abstract

Gestational diabetes mellitus (GDM) is a metabolically complex disease with major genetic determinants. GDM has been associated with insulin resistance and dysfunction of pancreatic beta cells, so the GDM candidate genes are those that encode proteins modulating the function and secretion of insulin, such as that for calpain 10 (CAPN10). This study aimed to assess whether single nucleotide polymorphism (SNP)-43, SNP-44, SNP-63, and the indel-19 variant, and specific haplotypes of the CAPN10 gene were associated with gestational diabetes mellitus. We studied 116 patients with gestational diabetes mellitus and 83 women with normal glucose tolerance. Measurements of anthropometric and biochemical parameters were performed. SNP-43, SNP-44, and SNP-63 were identified by polymerase chain reaction (PCR)-restriction fragment length polymorphisms, while the indel-19 variant was detected by TaqMan qPCR assays.  The allele, genotype, and haplotype frequencies of the four variants did not differ significantly between women with gestational diabetes mellitus and controls. However, in women with gestational diabetes mellitus, glucose levels were significantly higher bearing the 3R/3R genotype than in carriers of the 3R/2R genotype of the indel-19 variant (p = 0.006). In conclusion, the 3R/3R genotype of the indel-19 variant of the CAPN-10 gene influenced increased glucose levels in these Mexican women with gestational diabetes mellitus.

Keywords

CAPN10 variants Gestational diabetes Haplotypes Mexican women.
Castro-Martí­nez, A. G., Sánchez-Corona, J., Vázquez-Vargas, A. P., Garcí­a-Zapién, A. G., López-Quintero, A., Villalpando-Velazco, H. J., & Flores-Martí­nez, S. E. (2018). Association analysis of calpain 10 gene variants/haplotypes with gestational diabetes mellitus among Mexican women. Cellular and Molecular Biology, 64(3), 81–86. https://doi.org/10.14715/cmb/2018.64.3.13
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References
  1. Fuller KP, Borgida AF. Gestational diabetes mellitus screening using the one-step versus two-step method in a high-risk practice. Clin Diabetes 2014;32:148–50.
  2. Ramí­rez-Torres MA. The importance of gestational diabetes beyond pregnancy. Nutr Rev 2013;71:S37–S41.
  3. Di Cianni G, Miccoli R, Volpe L, Lencioni C, Del Prato S. Intermediate metabolism in normal pregnancy and in gestational diabetes. Diabetes Metab Res Rev 2003;19:259–70.
  4. Rice GE, Illanes SE, Mitchell MD. Gestational diabetes mellitus: a positive predictor of type 2 diabetes? Int J Endocrinol 2012 DOI:10.1155/2012/721653.
  5. Ben-Haroush A, Yogev Y, Hod M. Epidemiology of gestational diabetes mellitus and its association with type 2 diabetes. Diabet Med 2004;21:103–13.
  6. Leipold H, Knöfler M, Gruber C, Haslinger P, Bancher-Todesca D, Worda C. Calpain-10 haplotype combination and association with gestational diabetes mellitus. Obstet Gynecol 2004; 103:1235–40.
  7. Ferrara A. Increasing prevalence of gestational diabetes mellitus: a public health perspective. Diabetes Care 2007;30,141–6.
  8. Buchanan TA, Xiang AH. Gestational diabetes mellitus. J Clin Invest 2005;115:485–91.
  9. Horikawa Y, Oda N, Cox NJ, Li X, Orho-Melander M, Hara M, et al. Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus. Nat Genet 2000;26:163–75.
  11. Suzuki K, Hata S, Kawabata Y, Sorimachi H. Structure, activation, and biology of calpain. Diabetes 2004;53:12–8.
  12. Dong B, Liu R. Characterization of endogenous and recombinant human calpain-10. Biochimie 2008;90:1362–71.
  13. Ridderstråle M, Nilsson E. Type 2 diabetes candidate gene CAPN10: first, but not last. Curr Hypertens Rep 2008;10:19–24.
  14. Shaat N, Ekelund M, Lernmark A, Ivarsson S, Almgren P, Berntorp K, et al. Association of the E23K polymorphism in the KCNJ11 gene with gestational diabetes mellitus. Diabetologia 2005;48:2544–51.
  15. Luo YP, Xue YM, Zhao YQ. [Single-nucleotide polymorphisms and genotype combinations in calpain-10 gene of gestational diabetes mellitus]. Zhonghua Liu Xing Bing Xue Za Zhi 2009;30:101–2.
  16. Khan IA, Movva S, Shaik NA, Chava S, Jahan P, Mukkavali KK, et al. Investigation of Calpain 10 (rs2975760) gene polymorphism in Asian Indians with gestational diabetes mellitus. Meta Gene 2014;2:299–306.
  17. Neuhaus T, Graf C, Stier S, Knapp M, Grunewald E, Ko YD, et al. Variation in the Calpain-10 gene is not associated with gestational diabetes mellitus. Scand J Clin Lab Invest 2014;74:59–66.
  18. American Diabetes Association (ADA). Gestational diabetes mellitus. Diabetes Care 2004; 27:88–90.
  19. Gustincich S, Manfiolett G, Del Sal G, Schneider C, Carninci P. A fast method for high quality genomic DNA extraction from whole human blood. Biotechniques 1991;11:298–302.
  20. Orho-Melander M, Klannemark M, Svensson MK, Ridderstråle M, Lindgren C, Groop L. Variants in the calpain-10 gene predispose to insulin resistance and elevated free fatty acid levels. Diabetes 2002;51:2658–64.
  21. Daimon M, Oizumi T, Saitoh T, Kameda W, Yamaguchi H, Ohnuma H, et al. Calpain 10 gene polymorphisms are related, not to type 2 diabetes, but to increased serum cholesterol in Japanese. Diabetes Res Clin Pract 2002;56:147–52.
  22. Marshall C, Hitman GA, Partridge CJ, Clark A, Ma H, Shearer TR, et al. Evidence that an isoform of calpain-10 is a regulator of exocytosis in pancreatic beta-cells. Mol Endocrinol 2005;19:213–24.
  23. Turner MD, Fulcher FK, Jones CV, Smith BT, Aganna E, Partridge CJ, et al. Calpain facilitates actin reorganization during glucose-stimulated insulin secretion. Biochem Biophys Res Commun 2007;352:650–5.
  24. Cui J, Xu X, Yin S, Chen F, Li P, Song C. Meta-analysis of the association between four CAPN10 gene variants and gestational diabetes mellitus. Arch Gynecol Obstet 2016;294:447–53.
  25. Wu HR, Yang HX. [Association of the calpain-10 gene polymorphism with glucose metabolism disorder in pregnant women.] Zhonghua Fu Chan Ke Za Zhi 2009;44:183–7.
  26. Rubi-Castellanos R, Martí­nez-Cortés G, Muñoz-Valle JF, González-Martí­n A, Cerda-Flores RM, Anaya-Palafox M, et al. Pre-Hispanic Mesoamerican demography approximates the present-day ancestry of Mestizos throughout the territory of Mexico. Am J Phys Anthropol 2009;139:284–94.
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