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Vol. 63 No. 8: Issue 8

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New insights into the expression profile of MicroRNA-34c and P53 in infertile men spermatozoa and testicular tissue

https://doi.org/10.14715/cmb/2017.63.8.17
S. Rahbar
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
M. G. Novin
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
E. Alizadeh
Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Science, Tabriz, Iran
V. Shahnazi
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences Tabriz, Iran
F. Pashaei-Asl
Molecular Biology Laboratory, Biotechnology Research Center, Tabriz University of Medical Sciences,Tabriz, Iran
Y. A. AsrBadr
Urology Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
L. Farzadi
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences Tabriz, Iran
E. Ebrahimie
Department of Genetics and Evolution, School of Biological Sciences, the University of Adelaide, Adelaide, Australia
M. Pashaiasl
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Corresponding Author(s) : S. Rahbar

s.rahbar1393@gmail.com

Cellular and Molecular Biology, Vol. 63 No. 8: Issue 8

Abstract

Spermatogenesis is proliferation and differentiation processes of stem spermatogonia into mature spermatozoa controlled by the genes responsible for transcription and post transcription levels. MicroRNAs (miRNA) are  the key factors during gene expression in RNA silencing and post-transcriptional regulation. They play main roles in regulation of early and late spermatogenesis, and reproduction. In this study, we investigate the role of miRNAs in infertile males.
The patients were assigned to five groups based on semen analysis (n=55), including normozoospermic (N), moderate oligoasthenoteratozoospermic (MOAT), severe oligoasthenoteratozoospermic (SOAT), obstructive azoospermia (OA) and non-obstructive azoospermia (NOA). Quantitative RT-PCR was recruited to study the expression of miR-34c and tumor suppressor p53 gene. In addition, malondialdehyde (MDA) and DNA fragmentation was measured. Network analysis was performed using Pathway Studio web tool (Elsevier). Our results revealed statistically significant increased expression of miR-34c in moderate oligoasthenoteratozoospermic, non-obstructive azoospermia and an increased expression of p53 in MOAT, SOAT and NOA males. Also, the percentage of DNA fragmentation and oxidative stress was significantly higher in infertile groups (MOAT and SOAT) than other groups. These findings provide a novel molecular mechanism of gene regulation during cell-cycle and apoptosis in sperm, which gives a new regulatory insight into male infertility in terms of molecular diagnosis.

Keywords

Sperm MicroRNAs Normozoospermic Oligoasthenoteratozoospermia Pathway Studio.
Rahbar, S., Novin, M. G., Alizadeh, E., Shahnazi, V., Pashaei-Asl, F., AsrBadr, Y. A., Farzadi, L., Ebrahimie, E., & Pashaiasl, M. (2017). New insights into the expression profile of MicroRNA-34c and P53 in infertile men spermatozoa and testicular tissue. Cellular and Molecular Biology, 63(8), 77–83. https://doi.org/10.14715/cmb/2017.63.8.17
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